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Chow H. Lee
University of Northern British Columbia
18Publications
9H-index
290Citations
Publications 18
Newest
#1Sumreen JavedH-Index: 1
#2Wai Ming LiH-Index: 1
Last.Chow H. LeeH-Index: 9
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The aim of this study was to investigate the anti-inflammatory activity of a previously un-studied wild mushroom, Echinodontium tinctorium, collected from the forests of north-central British Columbia. The lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used to study the in vitro anti-inflammatory activity. The crude alkaline extract demonstrated potent anti-inflammatory activity, and was further purified using a “bio-activity-guided-purification” approach. The size-exclusion and ...
#1Sebastian Mackedenski (UNBC: University of Northern British Columbia)H-Index: 3
#2Chuyi Wang (UNBC: University of Northern British Columbia)H-Index: 1
Last.Chow H. Lee (UNBC: University of Northern British Columbia)H-Index: 9
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Insulin-like growth factor 2 mRNA-binding protein-1 (IMP1) has high affinity for KRAS mRNA, and it can regulate KRAS expression in cells. We first characterized the molecular interaction between IMP1 and KRAS mRNA. Using IMP1 variants with a point mutation in the GXXG motif at each KH domain, we showed that all KH domains play a critical role in the binding of KRAS RNA. We mapped the IMP1-binding sites on KRAS mRNA and show that IMP1 has the highest affinity for nts 1–185. Although it has lower ...
#1Ankush Barad (UNBC: University of Northern British Columbia)H-Index: 1
#2Sebastian Mackedenski (UNBC: University of Northern British Columbia)H-Index: 3
Last.Kerry B. Reimer (UNBC: University of Northern British Columbia)H-Index: 5
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Abstract A growth-inhibitory polysaccharide (GIPinv) was purified using size-exclusion and ion-exchange chromatography from the fourth sodium hydroxide extraction step of a fungus found in British Columbia. The fungus was genetically identified as a member of the Paxillus involutus complex. GIPinv has an average molecular weight of 229 kDa and is a heteroglycan composed of glucose (65.9%), galactose (20.8%), mannose (7.8%), fucose (3.2%) and xylose (2.3%). GC–MS methylation analysis suggests tha...
#1Kashif Mehmood (UNBC: University of Northern British Columbia)H-Index: 2
#2Daud Akhtar (UNBC: University of Northern British Columbia)H-Index: 1
Last.Chow H. Lee (UNBC: University of Northern British Columbia)H-Index: 9
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The stabilization of glioma-associated oncogene 1 (GLI1) mRNA by coding region determinant binding protein (CRD-BP) through the Wnt/ β -catenin signaling pathway is implicated in the proliferation of colorectal cancer and basal cell carcinoma. Here, we set out to characterize the physical interaction between CRD-BP and GLI1 mRNA so as to find inhibitors for such interaction. Studies using CRD-BP variants with a point mutation in the GXXG motif at each KH domain showed that KH1 and KH2 domain are...
#1Sebastian Mackedenski (UNBC: University of Northern British Columbia)H-Index: 3
#2Chow H. Lee (UNBC: University of Northern British Columbia)H-Index: 9
The Coding Region Determinant-Binding Protein (CRD-BP) is an RNA-binding protein which, among other functions, provides a protective effect for select target mRNA transcripts against degradation, effectively increasing their half-life leading to elevated level of gene expression. CRD-BP was first identified due to its inherent ability to bind to a specific coding region of c-myc mRNA. It has further been demonstrated to convey a protective effect to target transcripts, including GLI1, MITF, CD44...
#1Manbir Chohan (UNBC: University of Northern British Columbia)H-Index: 3
#2Sebastian Mackedenski (UNBC: University of Northern British Columbia)H-Index: 3
Last.Chow H. Lee (UNBC: University of Northern British Columbia)H-Index: 9
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Abstract Apurinic/apyrimidinic endonuclease 1 (APE1) is the predominant mammalian enzyme in DNA base excision repair pathway that cleaves the DNA backbone immediately 5′ to abasic sites. In addition to its abasic endonuclease activity, APE1 has 3′ phosphatase and 3′–5′ exonuclease activities against DNA. We recently identified APE1 as an endoribonuclease that preferentially cleaves at UA, UG, and CA sites in single-stranded regions of RNAs and can regulate c- myc mRNA level and half-life in cell...
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