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G. Feher
University of California, San Diego
119Publications
49H-index
8,975Citations
Publications 119
Newest
Published on Jan 1, 2007
Marco Flores15
Estimated H-index: 15
,
R.A. Isaacson22
Estimated H-index: 22
+ 3 AuthorsG. Feher49
Estimated H-index: 49
The geometry of the hydrogen bonds to the two carbonyl oxygens of the semiquinone Q �� in the reaction center (RC) from the photosynthetic purple bacterium Rhodobacter sphaeroides R-26 were determined by fitting a spin Hamiltonian to the data derived from 1 H and 2 H ENDOR spectroscopies at 35 GHz and 80 K. The experiments were performed on RCs in which the native Fe 21 (high spin) was replaced by diamagnetic Zn 21 to prevent spectral line broadening of the Q �� due to magnetic coupling with the...
Published on Jan 1, 2007in Biophysical Journal3.67
Marco Flores15
Estimated H-index: 15
(UCSD: University of California, San Diego),
R.A. Isaacson22
Estimated H-index: 22
(UCSD: University of California, San Diego)
+ 3 AuthorsG. Feher49
Estimated H-index: 49
(UCSD: University of California, San Diego)
Abstract The geometry of the hydrogen bonds to the two carbonyl oxygens of the semiquinone QA ⋅ ⁡ − in the reaction center (RC) from the photosynthetic purple bacterium Rhodobacter sphaeroides R-26 were determined by fitting a spin Hamiltonian to the data derived from 1 H and 2 H ENDOR spectroscopies at 35GHz and 80K. The experiments were performed on RCs in which the native Fe 2+ (high spin) was replaced by diamagnetic Zn 2+ to prevent spectral line broadening of the QA ⋅ ⁡ − due to magnetic co...
Published on May 1, 2006in Biophysical Journal3.67
Marco Flores15
Estimated H-index: 15
(UCSD: University of California, San Diego),
R.A. Isaacson22
Estimated H-index: 22
(UCSD: University of California, San Diego)
+ 3 AuthorsG. Feher49
Estimated H-index: 49
(UCSD: University of California, San Diego)
Hydrogen bonds are important in determining the structure and function of biomolecules. Of particular interest are hydrogen bonds to quinones, which play an important role in the bioenergetics of respiration and photosynthesis. In this work we investigated the hydrogen bonds to the two carbonyl oxygens of the semiquinone in the well-characterized reaction center from the photosynthetic bacterium Rhodobacter sphaeroides R-26. We used electron paramagnetic resonance and electron nuclear double res...
Published on May 1, 2005in Biochemistry2.95
Mark L. Paddock31
Estimated H-index: 31
,
C. Chang6
Estimated H-index: 6
+ 4 AuthorsOkamura My23
Estimated H-index: 23
The photosynthetic reaction center (RC) from purple bacteria converts light into chemical energy. Although the RC shows two nearly structurally symmetric branches, A and B, light-induced electron transfer in the native RC occurs almost exclusively along the A-branch to a primary quinone electron acceptor QA. Subsequent electron and proton transfer to a mobile quinone molecule QB converts it to a quinol, QBH2. We report the construction and characterization of a series of mutants in Rhodobacter s...
Published on Nov 27, 2003in FEBS Letters2.67
Mark L. Paddock31
Estimated H-index: 31
(UCSD: University of California, San Diego),
G. Feher49
Estimated H-index: 49
(UCSD: University of California, San Diego),
Melvin Y. Okamura33
Estimated H-index: 33
(UCSD: University of California, San Diego)
Abstract The focus of this minireview is to discuss the state of knowledge of the pathways and rates of proton transfer in the bacterial reaction center (RC) from Rhodobacter sphaeroides . Protons involved in the light driven catalytic reduction of a quinone molecule Q B to quinol Q B H 2 travel from the aqueous solution through well defined proton transfer pathways to the oxygen atoms of the quinone. Three main topics are discussed: (1) the pathways for proton transfer involving the residues: H...
Published on Nov 1, 2003in Chemical Physics1.82
Marco Flores15
Estimated H-index: 15
(UCSD: University of California, San Diego),
R.A. Isaacson22
Estimated H-index: 22
(UCSD: University of California, San Diego)
+ 2 AuthorsWolfgang Lubitz64
Estimated H-index: 64
(MPG: Max Planck Society)
Abstract ENDOR spectroscopy at 35 GHz and 80 K was used to study the radical anion of 1,4-benzoquinone (BQ) in water and various alcohols. BQ -d 4 in H 2 O and BQ -h 4 in D 2 O were investigated with the aim to obtain information on the hydrogen bonds between the quinone oxygen and the respective solvent. The observed spectra were analyzed using the GENDOR program for the simulation of orientationally selected powder ENDOR spectra. From the spectral simulations the hyperfine coupling and nuclear...
Published on Aug 1, 2003in Biochemistry2.95
Mark L. Paddock31
Estimated H-index: 31
,
Laura B. Sagle18
Estimated H-index: 18
+ 3 AuthorsOkamura My23
Estimated H-index: 23
The bacterial photosynthetic reaction center (RC) uses light energy to catalyze the reduction of a bound quinone molecule QB to quinol QBH2. In RCs from Rhodobacter sphaeroides the protons involved in this process come from the cytoplasm and travel through pathways that involve His-H126 and His-H128 located near the proton entry point. In this study, we measured the pH dependence from 4.5 to 8.5 of the binding of the proton transfer inhibitor Cd 2+ , which ligates to these surface His in the RC ...
Published on Dec 1, 2002in Biochemistry2.95
Mark L. Paddock31
Estimated H-index: 31
,
Pia Ädelroth28
Estimated H-index: 28
+ 2 AuthorsJ. T. Beatty14
Estimated H-index: 14
The bacterial reaction center (RC) converts light into chemical energy through the reduction of an internal quinone molecule QB to QBH2. In the native RC, proton transfer is coupled to electron transfer and is not rate-controlling. Consequently, proton transfer is not directly observable, and its rate was unknown. In this work, we present a method for making proton transfer rate-controlling, which enabled us to determine its rate. The imidazole groups of the His-H126 and His-H128 proton donors, ...
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