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Nancy C. Horton
University of Arizona
Restriction enzymeMolecular biologyChemistryDNABiochemistry
76Publications
19H-index
1,861Citations
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Publications 76
Newest
#1Smarajit PolleyH-Index: 6
#2Dmitry LyumkisH-Index: 18
Last. Nancy C. HortonH-Index: 19
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#1Chad K. Park (UA: University of Arizona)H-Index: 14
#2Nancy C. Horton (UA: University of Arizona)H-Index: 19
Enzyme filaments are defined as reversible, functional, linear self-assemblies of a single type of enzyme. Filamentation has recently emerged as a new mode of enzymatic regulation. In this Comment, we briefly introduce the diversity and functional consequences of enzyme filamentation. Filamentation is a relatively unknown mode of enzyme regulation, which increases functional diversity.
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#1Chad K. Park (UA: University of Arizona)H-Index: 14
#2Nancy C. Horton (UA: University of Arizona)H-Index: 19
Filament formation by non-cytoskeletal enzymes has been known for decades, yet only relatively recently has its wide-spread role in enzyme regulation and biology come to be appreciated. This comprehensive review summarizes what is known for each enzyme confirmed to form filamentous structures in vitro, and for the many that are known only to form large self-assemblies within cells. For some enzymes, studies describing both the in vitro filamentous structures and cellular self-assembly formation ...
4 CitationsSource
#1Smarajit Polley (Bose Institute)H-Index: 6
#2Dmitry Lyumkis (Salk Institute for Biological Studies)H-Index: 18
Last. Nancy C. Horton (UA: University of Arizona)H-Index: 19
view all 3 authors...
Summary Filament formation by enzymes is increasingly recognized as an important phenomenon with potentially unique regulatory properties and biological roles. SgrAI is an allosterically regulated type II restriction endonuclease that forms filaments with enhanced DNA cleavage activity and altered sequence specificity. Here, we present the cryoelectron microscopy (cryo-EM) structure of the filament of SgrAI in its activated configuration. The structural data illuminate the mechanistic origin of ...
2 CitationsSource
#1Smarajit Polley (Bose Institute)H-Index: 6
#2Dmitry Lyumkis (Salk Institute for Biological Studies)H-Index: 18
Last. Nancy C. Horton (UA: University of Arizona)H-Index: 19
view all 3 authors...
ABSTRACT Filament or run-on oligomer formation by enzymes is increasingly recognized as an important phenomenon with potentially unique regulatory properties and biological roles. SgrAI is an allosterically regulated type II restriction endonuclease that forms run-on oligomeric (ROO) filaments with enhanced DNA cleavage activity and altered sequence specificity. Here, we present the 3.5 A cryo-electron microscopy structure of the ROO filament of SgrAI bound to a mimic of cleaved primary site DNA...
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#1Peng Xu (KU: University of Kansas)H-Index: 4
#2Safder S. Ganaie (KU: University of Kansas)H-Index: 8
Last. Jian-MingQiu (KU: University of Kansas)H-Index: 31
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Human parvovirus B19 (B19V), a member of the genus Erythroparvovirus of the family Parvoviridae , is a small non-enveloped virus that has a single-stranded DNA (ssDNA) genome of 5.6 kilobases with two inverted terminal repeats (ITRs). B19V infection often results in severe hematological disorders and fetal death in humans. B19V replication follows a model of rolling hairpin-dependent DNA replication, in which the large non-structural protein NS1 introduces a site-specific single strand nick in t...
1 CitationsSource
#1Claudia Barahona (UA: University of Arizona)H-Index: 3
#2L. Emilia Basantes (UA: University of Arizona)H-Index: 3
Last. Nancy C. HortonH-Index: 19
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3 CitationsSource
#1Chad K. Park (UA: University of Arizona)H-Index: 14
#2Jonathan L. Sanchez (UA: University of Arizona)H-Index: 4
Last. Nancy C. Horton (UA: University of Arizona)H-Index: 19
view all 7 authors...
5 CitationsSource
#1Chad K. Park (UA: University of Arizona)H-Index: 14
#2Jonathan L. Sanchez (UA: University of Arizona)H-Index: 4
Last. Nancy C. Horton (UA: University of Arizona)H-Index: 19
view all 7 authors...
5 CitationsSource
#1Jonathan L. Sanchez (UA: University of Arizona)H-Index: 4
#2Zachary Romero (UA: University of Arizona)H-Index: 1
Last. Nancy C. HortonH-Index: 19
view all 5 authors...
Infection with human parvovirus B19 (B19V) has been associated with a myriad of illnesses, including erythema infectiosum (Fifth disease), hydrops fetalis, arthropathy, hepatitis, and cardiomyopathy, and also possibly the triggering of any number of different autoimmune diseases. B19V NS1 is a multidomain protein that plays a critical role in viral replication, with predicted nuclease, helicase, and gene transactivation activities. Herein, we investigate the biochemical activities of the nucleas...
5 CitationsSource
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