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Wakao Fukuda
Kwansei Gakuin University
35Publications
13H-index
412Citations
Publications 35
Newest
#1Wakao Fukuda (Kwansei Gakuin University)H-Index: 13
#2Yuka Yamori (Kwansei Gakuin University)H-Index: 1
Last.Shinsuke Fujiwara (Kwansei Gakuin University)H-Index: 30
view all 10 authors...
Branched-chain polyamine (BCPA) synthase (BpsA), encoded by the bpsA gene, is responsible for the biosynthesis of BCPA in the hyperthermophilic archaeon Thermococcus kodakarensis, which produces N4-bis(aminopropyl)spermidine and spermidine. Here, next-generation DNA sequencing and liquid chromatography–mass spectrometry (LC–MS) were used to perform transcriptomic and proteomic analyses of a T. kodakarensis strain (DBP1) lacking bpsA. Subsequently, the contributions of BCPA to gene transcription ...
1 CitationsSource
#1Ryota Hidese (Kwansei Gakuin University)H-Index: 8
#2Masataka Toyoda (Osaka University)
Last.Shinsuke Fujiwara (Kwansei Gakuin University)H-Index: 30
view all 12 authors...
Source
#1Yuka Yamori (Kwansei Gakuin University)H-Index: 1
#2Masafumi Hamakawa (Kwansei Gakuin University)H-Index: 1
Last.Shinsuke Fujiwara (Kwansei Gakuin University)H-Index: 30
view all 7 authors...
Branched-chain polyamines (BCPAs) are unique polycations found in (hyper)thermophiles. Thermococcus kodakarensis grows optimally at 85 °C and produces the BCPA N4-bis(aminopropyl)spermidine by sequential addition of decarboxylated S-adenosylmethionine (dcSAM) aminopropyl groups to spermidine (SPD) by BCPA synthase A (BpsA). The T. kodakarensis bpsA deletion mutant (DBP1) did not grow at temperatures at or above 93 °C, and grew at 90 °C only after a long lag period following accumulation of exces...
1 CitationsSource
#1Takashi Nishio (Dodai: Doshisha University)H-Index: 2
#2Yuko Yoshikawa (Dodai: Doshisha University)H-Index: 17
Last.Kenichi Yoshikawa (Dodai: Doshisha University)H-Index: 54
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9 CitationsSource
#1Takashi Nishio (Dodai: Doshisha University)H-Index: 2
#2Yuko Yoshikawa (Dodai: Doshisha University)H-Index: 17
Last.Kenichi Yoshikawa (Dodai: Doshisha University)H-Index: 54
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Source
#1Ai kanemuraH-Index: 1
#2Yuko YoshikawaH-Index: 17
Last.Kenichi YoshikawaH-Index: 54
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4 CitationsSource
#1Ryota Hidese (Kwansei Gakuin University)H-Index: 8
#2Wakao Fukuda (Rits: Ritsumeikan University)H-Index: 13
Last.Shinsuke Fujiwara (Kwansei Gakuin University)H-Index: 30
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2 CitationsSource
#1Seiji KomedaH-Index: 19
#2Hiroki YoneyamaH-Index: 6
Last.Kenichi YoshikawaH-Index: 54
view all 14 authors...
Derivatives of the highly antitumor-active compound [{cis-Pt(NH3)2}2(μ-OH)(μ-tetrazolato-N2,N3)]2+ (5-H-Y), which is a tetrazolato-bridged dinuclear platinum(II) complex, were prepared by substituting a linear alkyl chain moiety at C5 of the tetrazolate ring. The general formula for the derivatives is [{cis-Pt(NH3)2}2(μ-OH)(μ-5-R-tetrazolato-N2,N3)]2+, where R is (CH2)nCH3 and n = 0 to 8 (complexes 1–9). The cytotoxicity of complexes 1–4 in NCI-H460 human non-small-cell lung cancer cells decreas...
5 CitationsSource
#1Tamotsu Kanai (Kyoto University)H-Index: 23
#2Ayako Yasukochi (Kyoto University)H-Index: 2
Last.Haruyuki Atomi (Kyoto University)H-Index: 45
view all 7 authors...
The maturation of [NiFe]-hydrogenases requires a number of accessory proteins, which include hydrogenase-specific endopeptidases. The endopeptidases carry out the final cleavage reaction of the C-terminal regions of [NiFe]-hydrogenase large subunit precursors. The hyperthermophilic archaeon Thermococcus kodakarensis harbors two [NiFe]-hydrogenases, a cytoplasmic Hyh and a membrane-bound Mbh, along with two putative hydrogenase-specific endopeptidase genes. In this study, we carried out a genetic...
7 CitationsSource
#1Akira Muramatsu (Dodai: Doshisha University)H-Index: 3
#2Yuta Shimizu (Dodai: Doshisha University)H-Index: 2
Last.Kenichi Yoshikawa (Dodai: Doshisha University)H-Index: 54
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We studied the effect of branched-chain polyamines on the folding transition of genome-sized DNA molecules in aqueous solution by the use of single-molecule observation with fluorescence microcopy. Detailed morphological features of polyamine/DNA complexes were characterized by atomic force microscopy (AFM). The AFM observations indicated that branched-chain polyamines tend to induce a characteristic change in the higher-order structure of DNA by forming bridges or crosslinks between the segment...
13 CitationsSource
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