Hitoshi Hasegawa
Ehime University
Molecular biologyVirologyImmunologyMedicineBiology
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Publications 148
Prefractionation of complex mixtures of proteins derived from biological samples is indispensable for proteome analysis via top-down mass spectrometry (MS). Polyacrylamide gel electrophoresis (PAGE), which enables high-resolution protein separation based on molecular size, is a widely used technique in biochemical experiments and has the potential to be useful in sample fractionation for top-down MS analysis. However, the lack of a means to efficiently recover the separated proteins in-gel has a...
#1Shoichi Fukui (Nagasaki University)H-Index: 9
#1S. Fukui (Nagasaki University)H-Index: 2
Last. Yoshihiro Arimura (Kyorin University)H-Index: 16
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Objective: The complement cascade, especially the alternative pathway of complement, has been shown in basic research to be associated with anti-neutrophil cytoplasmic autoantibody (ANCA)-associated vasculitis (AAV). We aimed to elucidate relationships between serum complement components and clinical characteristics in AAV.Method: In a nationwide prospective cohort study (RemIT-JAV-RPGN), we measured the serum levels of C1q, C2, C3, C3b/iC3b, C4, C4b, C5, C5a, C9, factor B, factor D, factor H, f...
#1Takahiko Sugihara (Tokyo Medical and Dental University)H-Index: 13
#2Hitoshi Hasegawa (Ehime University)H-Index: 33
Last. Noriyoshi Ogawa (Hamamatsu University)H-Index: 21
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BACKGROUND: Relapses frequently occur in giant cell arteritis (GCA), and long-term glucocorticoid therapy is required. The identification of associated factors with poor treatment outcomes is important to decide the treatment algorithm of GCA. METHODS: We enrolled 139 newly diagnosed GCA patients treated with glucocorticoids between 2007 and 2014 in a retrospective, multi-center registry. Patients were diagnosed with temporal artery biopsy, 1990 American College of Rheumatology classification cr...
#1Mitsuaki IsobeH-Index: 49
#2Koichi Amano (Saitama Medical University)H-Index: 20
Last. Shin-ichi Momomura (Jichi Medical University)H-Index: 29
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1 CitationsSource
#1Jun Ishizaki (Ehime University)H-Index: 6
#2Takuya Matsumoto (Ehime University)H-Index: 8
Last. Hitoshi Hasegawa (Ehime University)H-Index: 33
view all 5 authors...
AbstractAnti-tumor necrosis factor (TNF)-α therapy has high efficacy even for severe or refractory Behcet’s disease (BD). Here, we present the first report of a BD patient who developed neuro-Behcet’s disease (NBD) with isolated longitudinal extensive transverse myelitis (LETM) during long-term anti-TNF-α therapy. A 67-year-old woman receiving infliximab (IFX; 6 mg/kg, every 8 weeks) for 5 years presented with acute right-side-dominant paraplegia, superficial sensory disturbance and bladder dysf...
#1Masahiro Okada (Ehime University)H-Index: 8
#2Koichiro Suemori (Ehime University)H-Index: 11
Last. Naohito Hato (Ehime University)H-Index: 25
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Abstract Objective To investigate treatment outcomes, hearing outcomes, and adverse effects of rituximab (RTX) for intractable otitis media with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (OMAAV). Methods Twenty-three patients who met the criteria proposed by the OMAAV study group were included. RTX was used for patients who had difficulty achieving induction of remission using glucocorticoids (GC) and intravenous cyclophosphamide (IVCY). Results Six patients were treated w...
1 CitationsSource
#1Hitoshi Hasegawa (Ehime University)H-Index: 33
#2Takuya Matsumoto (Ehime University)H-Index: 8
Dendritic cells (DCs) are a heterogeneous population playing a pivotal role in immune responses and tolerance. DCs promote immune tolerance by participating in the negative selection of autoreactive T cells in the thymus. Furthermore, to eliminate autoreactive T cells that have escaped thymic deletion, DCs also induce immune tolerance in the periphery through various mechanisms. Breakdown of these functions leads to autoimmune diseases. Moreover, DCs play a critical role in maintenance of homeos...
10 CitationsSource
#1Akinori Hara (Kanazawa University)H-Index: 15
#2Takashi Wada (Okayama University)H-Index: 1
Last. Yoshihiro ArimuraH-Index: 16
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Objective. The aim was to elucidate the prognosis and risk factors associated with relapse during longterm remission maintenance therapy for antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Methods. Patients with newly diagnosed AAV (n = 156) were registered in the Remission Induction Therapy in Japanese patients with ANCA-associated Vasculitides (RemIT-JAV) study, and among them, 83 patients who achieved remission were enrolled and followed up for 24 additional months in ...
1 CitationsSource
#1Erika Takemasa (Ehime University)H-Index: 4
#2Shuang Liu (Ehime University)H-Index: 7
Last. Hitoshi Hasegawa (Ehime University)H-Index: 33
view all 3 authors...
: Techniques employing monoclonal antibodies (mAbs) are widely used in the initial development phase of biologics. The usefulness of mAbs in basic RA research has been established based on their characteristics, including specificity of binding, homogeneity, and ability to be produced on a large scale. MAb immunoglobulins are the starting material for the generation of smaller antibody fragments and other engineered immunomodulatory antibodies. In this chapter, the basic hybridoma technique, whi...
: The primary goal in the treatment of rheumatoid arthritis (RA) should be achieving control of disease activity, prevention of structural damage in joints, and normalization of function. Therefore, reliable tools are necessary to evaluate RA. Here, we describe methods recently used to assess RA.