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Wagner Campos Otoni
Universidade Federal de Viçosa
227Publications
28H-index
2,531Citations
Publications 227
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#1Sérgio Heitor Sousa Felipe (UFV: Universidade Federal de Viçosa)H-Index: 3
Last.Wagner Campos Otoni (UFV: Universidade Federal de Viçosa)H-Index: 28
view all 8 authors...
Ultraviolet-B (UV-B) radiation is an elicitor of secondary metabolites in plant tissue culture, but the effects on 20-hydroxyecdysone (20E) are still unclear. The 20E may show biotechnological, pharmacological, medical, and agrochemical applicability. Here, we use Pfaffia glomerata, a medically important species, to understand the impacts of UV-B radiation on their physiological performance, the expression of key genes involved in the 20E biosynthesis, and the 20E content. Two accessions (A22 an...
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#1Carlos Hernán Barrera-Rojas (USP: University of São Paulo)
#2Gabriel Henrique Braga Rocha (ESALQ: Escola Superior de Agricultura Luiz de Queiroz)
Last.Fabio Tebaldi Silveira Nogueira (ESALQ: Escola Superior de Agricultura Luiz de Queiroz)H-Index: 20
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#1Perácio Rafael Bueno Ferreira (UFV: Universidade Federal de Viçosa)H-Index: 4
#2Ana Claudia Ferreira da Cruz (UFV: Universidade Federal de Viçosa)H-Index: 8
Last.Wagner Campos Otoni (UFV: Universidade Federal de Viçosa)H-Index: 28
view all 10 authors...
In vitro photoautotrophic propagation system has been successfully established for Pfaffia glomerata, a medicinal species that produces the phytoecdysteroid 20-hydroxyecdysone (20E) under forced ventilation and CO2 enrichment. For that, an adequate supporting material with high porosity in place of agar is required. In this study, we investigated metabolic and morpho-anatomical alterations of two accessions (Ac) of P. glomerata (Ac 22 and Ac 43) under photoautotrophy conditions, using two suppor...
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#1Kamila Motta de Castro (UFV: Universidade Federal de Viçosa)H-Index: 3
Last.Wagner Campos Otoni (UFV: Universidade Federal de Viçosa)H-Index: 28
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Interactions between circadian clock regulation and metabolic responses are believed to explain the importance of rhythmic behavior in plant growth and survival. Lippia alba is an important species because of the medicinal properties of its essential oil extract. The objective of this work was to evaluate the effect of photoperiod on anatomy, growth, essential oil profile, and the expression of genes related to the synthesis of monoterpenes, sesquiterpenes, and the circadian clock in L. alba gro...
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#1Daniele Vidal Faria (UFV: Universidade Federal de Viçosa)H-Index: 3
#2Ludmila Nayara de Freitas Correia (UFV: Universidade Federal de Viçosa)H-Index: 2
Last.Wagner Campos Otoni (UFV: Universidade Federal de Viçosa)H-Index: 28
view all 8 authors...
Abstract Light is a key factor influencing growth and development in plants. Specific irradiance and light quality can improve development and production of secondary compounds such as carotenoids during plant tissue culture. Bixin and norbixin, two apocarotenoids obtained from the seeds of Bixa orellana L. (annatto), are used as natural dyes in various industries. While annatto tissue culture has been successful, the effect of light in this species remains poorly understood. Here, we analyze fo...
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#1Tatiane Dulcineia Silva (UFV: Universidade Federal de Viçosa)H-Index: 3
#2Kristhiano Chagas (UFV: Universidade Federal de Viçosa)H-Index: 3
Last.Wagner Campos Otoni (UFV: Universidade Federal de Viçosa)H-Index: 28
view all 13 authors...
Pfaffia glomerata has potential pharmacological and medicinal properties due to the production of a secondary metabolite known as the phytoecdysteroid 20-hydroxyecdysone (20E). There have been increasing efforts for massive in vitro propagation of Pfaffia plants due to high extractivism and overharvesting of this species. Research on the species has shown that photoautotrophic cultivation can improve the production of 20E. In addition, other abiotic factors such as the formulations of culture me...
1 CitationsSource
#1Andreia Izabel Mikosvki (Universidade do Estado de Mato Grosso)
#2Nayara Tayane Silva (Universidade do Estado de Mato Grosso)H-Index: 1
Last.Maurecilne Lemes da Silva (Universidade do Estado de Mato Grosso)H-Index: 4
view all 8 authors...
The ornamental flower sector has growing over the past years worldwide with potential for further expansion. Among the ornamental plants, Passiflora species have been gaining ground in the market, mainly in European and North American countries. However, the market aiming the use of these species in ornamentation is still poorly explored. The inclusion of passion flower in the list of ornamental plants is related to the peculiar characteristics of the flower as it is complex structure, capacity ...
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#1Priscila Oliveira Silva (UFV: Universidade Federal de Viçosa)H-Index: 1
#2Diego Silva Batista (UFV: Universidade Federal de Viçosa)H-Index: 6
Last.Wagner Campos Otoni (UFV: Universidade Federal de Viçosa)H-Index: 28
view all 10 authors...
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Last.Wagner Campos Otoni (UFV: Universidade Federal de Viçosa)H-Index: 28
view all 10 authors...
The determination of the best reference gene is essential to improve and guarantee the accuracy of the qPCR technique. Thus, the objective of this work was to evaluate the normalization genes efficiency for qRT-PCR studies of Pfaffia glomerata, a species with marked medicinal and industrial interests due to the production of the phytoecdysteroid 20-hydroxyecdysone (20-E). We have selected four candidates as reference genes in P. glomerata: elongation factor-1α (EF-1α), glyceraldehyde 3-phosphate...
3 CitationsSource
#1Sérgio Heitor Sousa Felipe (UFV: Universidade Federal de Viçosa)H-Index: 3
Last.Wagner Campos Otoni (UFV: Universidade Federal de Viçosa)H-Index: 28
view all 13 authors...
Abstract – Salinity is a major threat to agriculture. However, depending on the concentration of soluble salts in soil, increased secondary metabolite levels can occur with no major damages to plant growth and development. The phytoecdysteroid (PE) 20-hydroxyecdysone (20E) is a secondary metabolite with biotechnological, medicinal, pharmaceutical and agrochemical applicability. Here, we characterize the responses (growth and physiology) of Pfaffia glomerata under different NaCl concentrations an...
1 CitationsSource
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