scinapse is loading now...
Ophir Shalem
Broad Institute
39Publications
18H-index
8,210Citations
Publications 39
Newest
Published on Aug 1, 2017in Nature 41.58
Shashank J. Patel11
Estimated H-index: 11
,
Neville E. Sanjana21
Estimated H-index: 21
+ 21 AuthorsTori N. Yamamoto12
Estimated H-index: 12
The authors describe a two-cell-type CRISPR screen to identify tumour-intrinsic genes that regulate the sensitivity of cancer cells to effector T cell function.
134 Citations Source Cite
Published on Mar 1, 2017in Nature 41.58
Michael A. Erb7
Estimated H-index: 7
,
Thomas G. Scott7
Estimated H-index: 7
+ 17 AuthorsDennis L. Buckley13
Estimated H-index: 13
ENL, identified in a genome-scale loss-of-function screen as a crucial requirement for proliferation of acute leukaemia, is required for leukaemic gene expression, and its YEATS chromatin-reader domain is essential for leukaemic growth.
52 Citations Source Cite
Published on Dec 1, 2016in European Journal of Cancer 7.19
Michael A. Erb7
Estimated H-index: 7
,
Georg E. Winter17
Estimated H-index: 17
+ 6 AuthorsJames E. Bradner73
Estimated H-index: 73
Source Cite
Published on Sep 30, 2016in Science 41.06
Neville E. Sanjana21
Estimated H-index: 21
(Broad Institute),
Jason Wright7
Estimated H-index: 7
(Broad Institute)
+ 6 AuthorsFeng Zhang104
Estimated H-index: 104
(Broad Institute)
The noncoding genome affects gene regulation and disease, yet we lack tools for rapid identification and manipulation of noncoding elements. We developed a CRISPR screen using ~18,000 single guide RNAs targeting >700 kilobases surrounding the genes NF1 , NF2 , and CUL3 , which are involved in BRAF inhibitor resistance in melanoma. We find that noncoding locations that modulate drug resistance also harbor predictive hallmarks of noncoding function. With a subset of regions at the CUL3 locus, we d...
100 Citations Source Cite
Published on Sep 1, 2016
Pierre Fontanillas27
Estimated H-index: 27
,
Neville E. Sanjana21
Estimated H-index: 21
+ 6 AuthorsFeng Zhang104
Estimated H-index: 104
Published on Apr 1, 2016
Isha H. Jain6
Estimated H-index: 6
,
L. Zazzeron9
Estimated H-index: 9
+ 11 AuthorsWarren M. Zapol70
Estimated H-index: 70
Published on Apr 1, 2016in Science 41.06
Isha H. Jain6
Estimated H-index: 6
(Broad Institute),
L. Zazzeron9
Estimated H-index: 9
(Harvard University)
+ 11 AuthorsNeville E. Sanjana21
Estimated H-index: 21
(Massachusetts Institute of Technology)
Defects in the mitochondrial respiratory chain (RC) underlie a spectrum of human conditions, ranging from devastating inborn errors of metabolism to aging. We performed a genome-wide Cas9-mediated screen to identify factors that are protective during RC inhibition. Our results highlight the hypoxia response, an endogenous program evolved to adapt to limited oxygen availability. Genetic or small-molecule activation of the hypoxia response is protective against mitochondrial toxicity in cultured c...
109 Citations Source Cite
Published on Dec 3, 2015in Blood 15.13
Daniel E. Bauer32
Estimated H-index: 32
(Harvard University),
Matthew C. Canver13
Estimated H-index: 13
(Harvard University)
+ 17 AuthorsDivya S. Vinjamur3
Estimated H-index: 3
(Harvard University)
Common genetic variation associated with fetal hemoglobin (HbF) level and β-hemoglobin disorder clinical severity marks an erythroid enhancer within the BCL11A gene. The 12 kb intronic enhancer contains three ~1 kb erythroid DNase I hypersensitive sites (DHSs), termed +55, +58, and +62. Here we utilized a human adult-stage erythroid cell line to show by CRISPR-Cas9 mediated targeted deletion that the composite enhancer is required both for BCL11A expression and HbF repression. Because deletion o...
4 Citations
Published on Nov 1, 2015in Nature 41.58
Matthew C. Canver13
Estimated H-index: 13
(Harvard University),
Elenoe C. Smith6
Estimated H-index: 6
(Harvard University)
+ 16 AuthorsSara P. Garcia4
Estimated H-index: 4
(Harvard University)
Enhancers, critical determinants of cellular identity, are commonly recognized by correlative chromatin marks and gain-of-function potential, although only loss-of-function studies can demonstrate their requirement in the native genomic context. Previously, we identified an erythroid enhancer of human BCL11A, subject to common genetic variation associated with the fetal haemoglobin level, the mouse orthologue of which is necessary for erythroid BCL11A expression. Here we develop pooled clustered...
309 Citations Source Cite
Published on Sep 1, 2015
Matthew C. Canver13
Estimated H-index: 13
,
Elenoe C. Smith6
Estimated H-index: 6
+ 16 AuthorsSidinh Luc19
Estimated H-index: 19
1234