Elisa Coluzzi
Sapienza University of Rome
Publications 18
#1Marco De VitisH-Index: 1
Last.Antonella SguraH-Index: 17
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Cancer cells need to acquire telomere maintenance mechanisms in order to counteract progressive telomere shortening due to multiple rounds of replication. Most human tumors maintain their telomeres expressing telomerase whereas the remaining 15%–20% utilize the alternative lengthening of telomeres (ALT) pathway. Previous studies have demonstrated that ionizing radiations (IR) are able to modulate telomere lengths and to transiently induce some of the ALT-pathway hallmarks in normal primary fibro...
#1Ilaria Laudadio (Sapienza University of Rome)H-Index: 4
#2Francesca Orso (UNITO: University of Turin)H-Index: 17
Last.Claudia Carissimi (Sapienza University of Rome)H-Index: 7
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Abstract Telomerase reverse transcriptase (TERT) and telomerase RNA component (TERC) constitute the core telomerase enzyme that maintains the length of telomeres. Telomere maintenance is affected in a broad range of cancer and degenerative disorders. Taking advantage of gain‐ and loss‐of‐function approaches, we show that Argonaute 2 (AGO2) promotes telomerase activity and stimulates the association between TERT and TERC. AGO2 depletion results in shorter telomeres as well as in lower proliferati...
#1Elisa ColuzziH-Index: 4
#2Stefano LeoneH-Index: 17
Last.Antonella SguraH-Index: 17
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Oxidative DNA damage, particularly 8-oxoguanine, represents the most frequent DNA damage in human cells, especially at the telomeric level. The presence of oxidative lesions in the DNA can hinder the replication fork and is able to activate the DNA damage response. In this study, we wanted to understand the mechanisms by which oxidative damage causes telomere dysfunction and senescence in human primary fibroblasts. After acute oxidative stress at telomeres, our data demonstrated a reduction in T...
#1Valeria FranchiniH-Index: 2
#2Silvio Ceccuzzi (ENEA)H-Index: 7
Last.Elisa Regalbuto (Sapienza University of Rome)H-Index: 4
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In this paper, we present a comprehensive discussion of the results obtained after in vitro exposure of human fetal fibroblasts and human adult fibroblasts to pulsed radiation in a wide band between 100 and 150 GHz and to continuous wave radiation at 25 GHz. In order to assess potential effects of exposure, the genome integrity, cell cycle, cytological ultrastructure, and proteins expression were evaluated.
#1Elvira ParrottaH-Index: 5
Last.Antonella Sgura (Sapienza University of Rome)H-Index: 17
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Human pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells, hold enormous promise for many biomedical applications, such as regenerative medicine, drug testing, and disease modeling. Although induced pluripotent stem cells resemble embryonic stem cells both morphologically and functionally, the extent to which these cell lines are truly equivalent, from a molecular point of view, remains controversial. Principal component analysis and K-means cluster analysis...
#1Francesco Berardinelli (INFN: Istituto Nazionale di Fisica Nucleare)H-Index: 12
#2Elisa ColuzziH-Index: 4
Last.Antonio Antoccia (INFN: Istituto Nazionale di Fisica Nucleare)H-Index: 23
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Abstract One of the hallmarks of cancer consists in the ability of tumor cells to divide indefinitely, and to maintain stable telomere lengths throughout the activation of specific telomere maintenance mechanisms (TMM). Therefore in the last fifteen years, researchers proposed to target telomerase or telomeric structure in order to block limitless replicative potential of cancer cells providing a fascinating strategy for a broad-spectrum cancer therapy. In the present review, we report in vitro ...