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Theodoros Goulas
Spanish National Research Council
Crystal structureChemistryPorphyromonas gingivalisBiochemistryBiology
44Publications
8H-index
290Citations
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Publications 46
Newest
#1Soraia R Mendes (CSIC: Spanish National Research Council)
#2Laura del Amo-Maestro (CSIC: Spanish National Research Council)
Last. F. Xavier Gomis-Rüth (CSIC: Spanish National Research Council)H-Index: 31
view all 6 authors...
Matrix metalloproteinases (MMPs) occur in 23 human paralogues with key functions in physiology, and their activity is controlled by protein inhibitors. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK), which is essential for embryogenesis and tumour suppression, has been reported to inhibit MMPs. Here, we developed eukaryotic and bacterial expression systems for different RECK variants and analysed their inhibitory capacity against representative MMPs in vitro. We could not dete...
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#1L. Del Amo-MaestroH-Index: 1
Last. F.X. Gomis-RuthH-Index: 1
view all 4 authors...
Transforming growth factor β is a disulfide-linked dimeric cytokine that occurs in three highly related isoforms (TGFβ1–TGFβ3) engaged in signaling functions through binding of cognate TGFβ receptors. To regulate this pathway, the cytokines are biosynthesized as inactive pro-TGFβs with an N-terminal latency-associated protein preceding the mature moieties. Due to their pleiotropic implications in physiology and pathology, TGFβs are privileged objects of in vitro studies. However, such studies ha...
1 CitationsSource
#1Laura Marino-Puertas (CSIC: Spanish National Research Council)H-Index: 1
#2Laura del Amo-Maestro (CSIC: Spanish National Research Council)
Last. Theodoros Goulas (CSIC: Spanish National Research Council)H-Index: 8
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α2-Macroglobulins (α2Ms) regulate peptidases, hormones and cytokines. Mediated by peptidase cleavage, they transit between native, intact forms and activated, induced forms. α2Ms have been studied over decades using authentic material from primary sources, which was limited by sample heterogeneity and contaminants. Here, we developed high-yield expression systems based on transient transfection in Drosophila Schneider 2 and human Expi293F cells, which produced pure human α2M (hα2M) at ~1.0 and ~...
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#1Grzegorz Bereta (Jagiellonian University)H-Index: 2
#2Theodoros Goulas (CSIC: Spanish National Research Council)H-Index: 8
Last. F. Xavier Gomis-Rüth (CSIC: Spanish National Research Council)H-Index: 31
view all 7 authors...
2 CitationsSource
#1Anna Cuppari (CSIC: Spanish National Research Council)H-Index: 3
#2H. Korschgen (University of Mainz)H-Index: 2
Last. F.X. Gomis-Ruth (CSIC: Spanish National Research Council)H-Index: 1
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Mammalian fetuin-A and fetuin-B are abundant serum proteins with pleiotropic functions. Fetuin-B is a highly selective and potent inhibitor of metallo­peptidases (MPs) of the astacin family, which includes ovastacin in mammals. By inhibiting ovastacin, fetuin-B is essential for female fertility. The crystal structure of fetuin-B was determined unbound and in complex with archetypal astacin, and it was found that the inhibitor has tandem cystatin-type modules (CY1 and CY2). They are connected by ...
1 CitationsSource
#1Grzegorz Bereta (Jagiellonian University)H-Index: 2
#2Theodoros Goulas (CSIC: Spanish National Research Council)H-Index: 8
Last. F.X. Gomis-Ruth (CSIC: Spanish National Research Council)H-Index: 1
view all 7 authors...
1 CitationsSource
#1Joan L. ArolasH-Index: 17
#2Theodoros GoulasH-Index: 8
Last. F. Xavier Gomis-RüthH-Index: 31
view all 4 authors...
Metallopeptidases cleave polypeptides bound in the active-site cleft of catalytic domains through a general base/acid mechanism. This involves a solvent molecule bound to a catalytic zinc and general regulation of the mechanism through zymogen-based latency. Sixty reported structures from 11 metallopeptidase families reveal that prosegments, mostly N-terminal of the catalytic domain, block the cleft regardless of their size. Prosegments may be peptides (5–14 residues), which are only structured ...
3 CitationsSource
Abstract The matrix metalloproteinase (MMP) family belongs to the metzincin clan of zinc-dependent metallopeptidases. Due to their enormous implications in physiology and disease, MMPs have mainly been studied in vertebrates. They are engaged in extracellular protein processing and degradation, and present extensive paralogy, with 23 forms in humans. One characteristic of MMPs is a ~ 165-residue catalytic domain (CD), which has been structurally studied for 14 MMPs from human, mouse, rat, pig an...
1 CitationsSource
#1R. Garcia-Castellanos (CSIC: Spanish National Research Council)H-Index: 10
#2Nadia Sukusu Nielsen (AU: Aarhus University)H-Index: 4
Last. F. Xavier Gomis-Rüth (CSIC: Spanish National Research Council)H-Index: 31
view all 9 authors...
Summary A major cause of visual impairment, corneal dystrophies result from accumulation of protein deposits in the cornea. One of the proteins involved is transforming growth factor β-induced protein (TGFBIp), an extracellular matrix component that interacts with integrins but also produces corneal deposits when mutated. Human TGFBIp is a multi-domain 683-residue protein, which contains one CROPT domain and four FAS1 domains. Its structure spans ∼120 A and reveals that vicinal domains FAS1-1/FA...
7 CitationsSource
#1Theodoros GoulasH-Index: 8
#2I. Garcia-FerrerH-Index: 6
Last. F. Xavier Gomis-RüthH-Index: 31
view all 6 authors...
This study was supported in part by grants from European, Spanish, and Catalan agencies (grant references FP7-HEALTH-2012-306029-2 ‘TRIGGER’, BFU2015-64487R, MDM-2014-0435, BIO2013-49320-EXP, and 2014SGR9). The Structural Biology Unit (www.sbu.csic.es) of IBMB has been named a ‘Maria de Maeztu’ Unit of Excellence by the Spanish Ministry of Economy and Competitiveness. T.G. and I.G.-F. acknowledge a ‘Juan-dela-Cierva’ contract from the Spanish Ministry of Economy and Competitiveness (JCI-2012-135...
7 CitationsSource
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