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Ofra Sabag
Hebrew University of Jerusalem
Fusion proteinMolecular biologyDNA methylationMethylationBiology
12Publications
7H-index
160Citations
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Publications 12
Newest
#1Tal Falick Michaeli (HUJI: Hebrew University of Jerusalem)H-Index: 2
#2Adam Spiro (HUJI: Hebrew University of Jerusalem)H-Index: 8
Last. Yuval Gielchinsky (HUJI: Hebrew University of Jerusalem)H-Index: 10
view all 9 authors...
: Gestational age determination by traditional tools (last menstrual period, ultrasonography measurements and Ballard Maturational Assessment in newborns) has major limitations and therefore there is a need to find different approaches. In this study, we looked for a molecular marker that can be used to determine the accurate gestational age of the newborn. To this end, we performed reduced representation bisulfite sequencing (RRBS) on 41 cord blood and matching placenta samples from women betwe...
1 CitationsSource
#1Razi Greenfield (HUJI: Hebrew University of Jerusalem)H-Index: 2
#2Amalia Tabib (HUJI: Hebrew University of Jerusalem)H-Index: 5
Last. Howard Cedar (HUJI: Hebrew University of Jerusalem)H-Index: 73
view all 7 authors...
Following erasure in the blastocyst, the entire genome undergoes de novo methylation at the time of implantation, with CpG islands being protected from this process. This bimodal pattern is then preserved throughout development and the lifetime of the organism. Using mouse embryonic stem cells as a model system, we demonstrate that the binding of an RNA polymerase complex on DNA before de novo methylation is predictive of it being protected from this modification, and tethering experiments demon...
2 CitationsSource
#1Yitzhak Reizel (UPenn: University of Pennsylvania)H-Index: 3
#2Ofra Sabag (HUJI: Hebrew University of Jerusalem)H-Index: 7
Last. Howard Cedar (HUJI: Hebrew University of Jerusalem)H-Index: 73
view all 15 authors...
Development in mammals is accompanied by specific de novo and demethylation events that are thought to stabilize differentiated cell phenotypes. We demonstrate that a large percentage of the tissue-specific methylation pattern is generated postnatally. Demethylation in the liver is observed in thousands of enhancer-like sequences associated with genes that undergo activation during the first few weeks of life. Using. conditional gene ablation strategy we show that the removal of these methyl gro...
12 CitationsSource
#1Yitzhak Reizel (HUJI: Hebrew University of Jerusalem)H-Index: 3
#2Adam Spiro (Weizmann Institute of Science)H-Index: 8
Last. Howard Cedar (HUJI: Hebrew University of Jerusalem)H-Index: 73
view all 8 authors...
DNA methylation patterns are set up in a relatively fixed programmed manner during normal embryonic development and are then stably maintained. Using genome-wide analysis, we discovered a postnatal pathway involving gender-specific demethylation that occurs exclusively in the male liver. This demodification is programmed to take place at tissue-specific enhancer sequences, and our data show that the methylation state at these loci is associated with and appears to play a role in the transcriptio...
28 CitationsSource
#1Ofra Sabag (HUJI: Hebrew University of Jerusalem)H-Index: 7
#2Haya Lorberboum-Galski (HUJI: Hebrew University of Jerusalem)H-Index: 21
The discovery and therapeutic use of antibiotics made a major contribution to the reduction of human morbidity and mortality. However, the growing resistance to antibiotics has become a matter of huge concern. In this study we aimed to develop an innovative approach to treat bacterial infections utilizing two components: the human antibacterial peptide β-defensin-3 (BD3) and the bacterial protein flagellin (F). This combination was designed to provide an efficient weapon against bacterial infect...
1 CitationsSource
#1Ofra SabagH-Index: 7
#2Ayelet ZamirH-Index: 1
Last. Howard CedarH-Index: 73
view all 8 authors...
Using embryonic stem cells as a model system for studying the basic bimodal DNA methylation pattern in vivo, a new study now indicates that demethylation is not required for generating unmethylated regions such as CpG islands as part of the overall bimodal methylation pattern but is involved in resetting methylation patterns during somatic-cell reprogramming.
23 CitationsSource
#1Rotem Kedar (HUJI: Hebrew University of Jerusalem)H-Index: 2
#2Ofra Sabag (HUJI: Hebrew University of Jerusalem)H-Index: 7
Last. Haya Lorberboum-Galski (HUJI: Hebrew University of Jerusalem)H-Index: 21
view all 4 authors...
BACKGROUND: A wide range of hematologic malignancies arises from numerous cell types. In an attempt to offer a new target for treating B-cell malignancies, in this study, the authors tested the possibility of using the CD40/CD40L system as a common targeting system for the various malignancies in this group. METHODS: Two chimeric proteins, soluble CD40 ligand (sCD40L)-caspase 3 (sCD40L-l-Caspase3) and sCD40L-pseudomonas exotoxin 38 (PE38) (sCD40L-l-PE38), were constructed, expressed, and partial...
7 CitationsSource
#1Yitav Glantz (HUJI: Hebrew University of Jerusalem)
#2Ofra Sabag (HUJI: Hebrew University of Jerusalem)H-Index: 7
Last. Haya Lorberboum-Galski (HUJI: Hebrew University of Jerusalem)H-Index: 21
view all 5 authors...
Designing a chimeric protein and developing a procedure for its stable production as a biologically active protein, are key steps in its potential application to clinical trails. IL2-Caspase3 chimeric protein designed to target activated T lymphocytes was found to be a promising molecule for targeted treatment, however was found to be difficult to produce as a biological active molecule. Thus, we designed a new version of the molecule, IL2-Caspase3s, in which six amino acids (aa 29–34) from the ...
Source
#1Eitan Israeli (HUJI: Hebrew University of Jerusalem)H-Index: 8
#2Eugenia Yakunin (HUJI: Hebrew University of Jerusalem)H-Index: 5
Last. Ronit Sharon (HUJI: Hebrew University of Jerusalem)H-Index: 14
view all 12 authors...
Alpha Synuclein (α-Syn) is a protein implicated in mechanisms of neuronal degeneration in Parkinson's disease (PD). α-Syn is primarily a neuronal protein, however, its expression is found in various tumors including ovarian, colorectal and melanoma tumors. It has been hypothesized that neurodegeneration may share common mechanisms with oncogenesis. We tested whether α-Syn expression affects tumorigenesis of three types of tumors. Specifically, B16 melanoma, E0771 mammary gland adenocarcinoma and...
12 CitationsSource
#1Matan Rapoport (HUJI: Hebrew University of Jerusalem)H-Index: 5
#2Lina Salman (HUJI: Hebrew University of Jerusalem)H-Index: 1
Last. Haya Lorberboum-Galski (HUJI: Hebrew University of Jerusalem)H-Index: 21
view all 5 authors...
Medicine today offers no cure for patients suffering from mitochondrial disorders, such as lipoamide dehydrogenase (LAD; also known as E3) deficiency, and treatment is limited to symptomatic care. LAD is one of the components of the α-ketoacid dehydrogenase complexes, which are mitochondrial multienzyme complexes crucial for the metabolism of carbohydrates and amino acids. Recently, we tested the therapeutic approach for treating mitochondrial disorders whereby the activity of multicomponent com...
28 CitationsSource
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