Michael J. Ophir
Tufts University
Molecular biologySignal transducing adaptor proteinT-cell receptorBiologyCell biology
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Publications 3
#1Juliana B. Lewis (Tufts University)H-Index: 1
#2Frank A. Scangarello (Tufts University)H-Index: 1
Last. Stephen C. Bunnell (Tufts University)H-Index: 18
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Antigen recognition by the T cell receptor (TCR) directs the assembly of essential signaling complexes known as SLP-76 microclusters. Here, we show that the interaction of the adhesion and degranulation-promoting adaptor protein (ADAP) with SLP-76 enables the formation of persistent microclusters and the stabilization of T cell contacts, promotes integrin-independent adhesion, and enables the upregulation of CD69. Using point mutants and a novel phospho-specific antibody, we show that Y595 is es...
1 CitationsSource
#1Michael J. Ophir (Tufts University)H-Index: 1
#2Beiyun C. Liu (Tufts University)H-Index: 6
Last. Stephen C. Bunnell (Tufts University)H-Index: 18
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The T cell receptor (TCR) triggers the assembly of “SLP-76 microclusters,” which mediate signals required for T cell activation. In addition to regulating integrin activation, we show that Src kinase–associated phosphoprotein of 55 kD (SKAP55) is required for microcluster persistence and movement, junctional stabilization, and integrin-independent adhesion via the TCR. These functions require the dimerization of SKAP55 and its interaction with the adaptor adhesion and degranulation-promoting ada...
9 CitationsSource
#1Michael J. OphirH-Index: 1
#2Ken NguyenH-Index: 2
Last. Stephen C. BunnellH-Index: 18
view all 3 authors...