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Ioannis Karakikes
Cardiovascular Institute of the South
86Publications
26H-index
1,846Citations
Publications 86
Newest
#1Karina H. Nakayama (Cardiovascular Institute of the South)H-Index: 9
#2Marco Quarta (Stanford University)H-Index: 11
Last.Thomas A. Rando (Stanford University)H-Index: 68
view all 10 authors...
Traumatic skeletal muscle injuries cause irreversible tissue damage and impaired revascularization. Engineered muscle is promising for enhancing tissue revascularization and regeneration in injured muscle. Here we fabricated engineered skeletal muscle composed of myotubes interspersed with vascular endothelial cells using spatially patterned scaffolds that induce aligned cellular organization, and then assessed their therapeutic benefit for treatment of murine volumetric muscle loss. Murine skel...
#1Jaecheol LeeH-Index: 10
#2Vittavat Termglinchan (Cardiovascular Institute of the South)H-Index: 9
Last.Haodi Wu (Cardiovascular Institute of the South)H-Index: 18
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Lamin A/C (LMNA) is one of the most frequently mutated genes associated with dilated cardiomyopathy (DCM). DCM related to mutations in LMNA is a common inherited cardiomyopathy that is associated with systolic dysfunction and cardiac arrhythmias. Here we modelled the LMNA-related DCM in vitro using patient-specific induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). Electrophysiological studies showed that the mutant iPSC-CMs displayed aberrant calcium homeostasis that led to arrhyt...
#1Timon Seeger (Stanford University)H-Index: 10
#2Rajani Shrestha (Stanford University)H-Index: 3
Last.Matthew Greenhaw (Stanford University)H-Index: 1
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Background: Hypertrophic cardiomyopathy (HCM) is frequently caused by mutations in myosin-binding protein C3 (MYBPC3) resulting in a premature termination codon (PTC). The underlying mechanisms of ...
#1Alex C.Y. Chang (Stanford University)H-Index: 9
#2Andrew H. Chang (Stanford University)H-Index: 5
Last.Timon Seeger (Stanford University)H-Index: 10
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This study demonstrates that significantly shortened telomeres are a hallmark of cardiomyocytes (CMs) from individuals with end-stage hypertrophic cardiomyopathy (HCM) or dilated cardiomyopathy (DCM) as a result of heritable defects in cardiac proteins critical to contractile function. Positioned at the ends of chromosomes, telomeres are DNA repeats that serve as protective caps that shorten with each cell division, a marker of aging. CMs are a known exception in which telomeres remain relativel...
#1Balpreet Kaur (Stanford University)
#2Isaac Perea-Gil (Stanford University)H-Index: 10
Last.Ioannis Karakikes (Stanford University)H-Index: 26
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Purpose of Review This review describes the recent progress in nuclease-based therapeutic applications for inherited heart diseases in vitro, highlights the development of the most recent genome editing technologies and discusses the associated challenges for clinical translation.
#1Jaecheol Lee (Stanford University)H-Index: 10
#2Ning-Yi Shao (Stanford University)H-Index: 16
Last.Ming-Tao Zhao (Stanford University)H-Index: 11
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Summary Cardiac development requires coordinated and large-scale rearrangements of the epigenome. The roles and precise mechanisms through which specific epigenetic modifying enzymes control cardiac lineage specification, however, remain unclear. Here we show that the H3K4 methyltransferase SETD7 controls cardiac differentiation by reading H3K36 marks independently of its enzymatic activity. Through chromatin immunoprecipitation sequencing (ChIP-seq), we found that SETD7 targets distinct sets of...
#1Oscar J. AbilezH-Index: 24
#2Evangeline Tzatzalos (Stanford University)H-Index: 4
Last.Praveen Shukla (Stanford University)H-Index: 13
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Background: The ability to differentiate human pluripotent stem cells (hPSCs) into cardiomyocytes (CMs) makes them an attractive source for repairing injured myocardium, disease modeling, and drug testing. Although current differentiation protocols yield hPSC-CMs to >90% efficiency, hPSC-CMs exhibit immature characteristics. With the goal of overcoming this limitation, we tested the effects of varying passive stretch on engineered heart muscle (EHM) structural and functional maturation, guided b...
#1Timon SeegerH-Index: 10
#2Caressa ChenH-Index: 2
Last.Joseph C. WuH-Index: 87
view all 4 authors...
#1Ming Tao Zhao (Stanford University)H-Index: 2
#2Haodong Chen (Stanford University)H-Index: 11
Last.Youngkyun Kim (Stanford University)H-Index: 9
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Abstract Patient-specific pluripotent stem cells (PSCs) can be generated via nuclear reprogramming by transcription factors (i.e., induced pluripotent stem cells, iPSCs) or by somatic cell nuclear transfer (SCNT). However, abnormalities and preclinical application of differentiated cells generated by different reprogramming mechanisms have yet to be evaluated. Here we investigated the molecular and functional features, and drug response of cardiomyocytes (PSC-CMs) and endothelial cells (PSC-ECs)...
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