Ioannis Karakikes
Cardiovascular Institute of the South
Induced pluripotent stem cellCardiologyStem cellMedicineBiology
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Publications 92
#1Lei Tian (Stanford University)H-Index: 4
#2Angelos Oikonomopoulos (Stanford University)H-Index: 6
Last. Nazish Sayed (Stanford University)H-Index: 15
view all 11 authors...
#1Thomas J. LaRocca (Stanford University)H-Index: 18
#2Timon Seeger (Stanford University)H-Index: 10
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 11 authors...
Background: MicroRNAs are small, noncoding RNAs that play a key role in gene expression. Accumulating evidence suggests that aberrant microRNA expression contributes to the heart failure (HF) pheno...
#1Arne A. N. Bruyneel (Stanford University)H-Index: 6
#2Alexandre R. Colas (DI: Discovery Institute)H-Index: 8
Last. Mark Mercola (Stanford University)H-Index: 50
view all 4 authors...
Gene editing strategies, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9), are revolutionizing biology. However, quantitative and sensitive detection of targeted mutations are required to evaluate and quantify the genome editing outcomes. Here we present AlleleProfileR, a new analysis tool, written in a combination of R and C++, with the ability to batch process the sequ...
#1Jae Cheol LeeH-Index: 23
#2Vittavat Termglinchan (Cardiovascular Institute of the South)H-Index: 9
Last. Joseph C. Wu (Cardiovascular Institute of the South)H-Index: 91
view all 24 authors...
Lamin A/C (LMNA) is one of the most frequently mutated genes associated with dilated cardiomyopathy (DCM). DCM related to mutations in LMNA is a common inherited cardiomyopathy that is associated with systolic dysfunction and cardiac arrhythmias. Here we modelled the LMNA-related DCM in vitro using patient-specific induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). Electrophysiological studies showed that the mutant iPSC-CMs displayed aberrant calcium homeostasis that led to arrhyt...
5 CitationsSource
#1Karina H. Nakayama (Cardiovascular Institute of the South)H-Index: 10
#2Marco Quarta (Stanford University)H-Index: 12
Last. Ngan F. Huang (Cardiovascular Institute of the South)H-Index: 31
view all 11 authors...
Traumatic skeletal muscle injuries cause irreversible tissue damage and impaired revascularization. Engineered muscle is promising for enhancing tissue revascularization and regeneration in injured muscle. Here we fabricated engineered skeletal muscle composed of myotubes interspersed with vascular endothelial cells using spatially patterned scaffolds that induce aligned cellular organization, and then assessed their therapeutic benefit for treatment of murine volumetric muscle loss. Murine skel...
1 CitationsSource
#1Pooja Nair (Stanford University)
#2Maricela Prado (Stanford University)
Last. Ioannis Karakikes (Stanford University)H-Index: 27
view all 4 authors...
#1Timon Seeger (Stanford University)H-Index: 10
#2Rajani Shrestha (Stanford University)H-Index: 4
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 19 authors...
Background: Hypertrophic cardiomyopathy (HCM) is frequently caused by mutations in myosin-binding protein C3 (MYBPC3) resulting in a premature termination codon (PTC). The underlying mechanisms of ...
4 CitationsSource
#1Alex C. Y. Chang (Stanford University)H-Index: 10
#2Andrew Chang (Stanford University)H-Index: 5
Last. Helen M. Blau (Stanford University)H-Index: 91
view all 18 authors...
This study demonstrates that significantly shortened telomeres are a hallmark of cardiomyocytes (CMs) from individuals with end-stage hypertrophic cardiomyopathy (HCM) or dilated cardiomyopathy (DCM) as a result of heritable defects in cardiac proteins critical to contractile function. Positioned at the ends of chromosomes, telomeres are DNA repeats that serve as protective caps that shorten with each cell division, a marker of aging. CMs are a known exception in which telomeres remain relativel...
8 CitationsSource
#1Balpreet Kaur (Stanford University)
#2Isaac Perea-Gil (Stanford University)H-Index: 11
Last. Ioannis Karakikes (Stanford University)H-Index: 27
view all 3 authors...
Purpose of Review This review describes the recent progress in nuclease-based therapeutic applications for inherited heart diseases in vitro, highlights the development of the most recent genome editing technologies and discusses the associated challenges for clinical translation.
#1Jae Cheol Lee (Stanford University)H-Index: 23
#2Ningyi Shao (Stanford University)H-Index: 19
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 15 authors...
Summary Cardiac development requires coordinated and large-scale rearrangements of the epigenome. The roles and precise mechanisms through which specific epigenetic modifying enzymes control cardiac lineage specification, however, remain unclear. Here we show that the H3K4 methyltransferase SETD7 controls cardiac differentiation by reading H3K36 marks independently of its enzymatic activity. Through chromatin immunoprecipitation sequencing (ChIP-seq), we found that SETD7 targets distinct sets of...
4 CitationsSource