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Olga I. Lavrik
Russian Academy of Sciences
Molecular biologyDNA polymeraseDNABiochemistryBiology
353Publications
25H-index
2,832Citations
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#1Olga I. Lavrik (RAS: Russian Academy of Sciences)H-Index: 25
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#1Svetlana N. Khodyreva (RAS: Russian Academy of Sciences)H-Index: 18
#2Olga I. Lavrik (NSU: Novosibirsk State University)H-Index: 25
Abstract Apurinic/apyrimidinic (AP) sites are widespread lesions in genomic DNA, arising from a number of exogenous and endogenous sources. These DNA lesions are highly mutagenic and demand efficient repair. The review is devoted to data on searching for previously unrecognized proteins capable of interaction with intact or cleaved AP sites. We mainly focused on proteins that form Schiff base upon this interaction. It is important to note that the aldehyde at the deoxyribose C1 atom both in inta...
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#1N. A. Moor (RAS: Russian Academy of Sciences)H-Index: 10
#2Inna A. Vasil'eva (RAS: Russian Academy of Sciences)H-Index: 8
Last. Olga I. Lavrik (RAS: Russian Academy of Sciences)H-Index: 25
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Human apurinic/apyrimidinic endonuclease 1 (APE1) has multiple functions in base excision DNA repair (BER) and other cellular processes. Its eukaryote-specific N-terminal extension plays diverse regulatory roles in interaction with different partners. Here, we explored its involvement in interaction with canonical BER proteins. Using fluorescence based-techniques, we compared binding affinities of the full-length and N-terminally truncated forms of APE1 (APE1NΔ35 and APE1NΔ61) for functionally a...
1 CitationsSource
#1D. K. Nilov (MSU: Moscow State University)H-Index: 5
#2N. V. Maluchenko (MSU: Moscow State University)H-Index: 7
Last. Olga I. Lavrik (RAS: Russian Academy of Sciences)H-Index: 25
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7-Methylguanine (7-MG), a natural compound that inhibits DNA repair enzyme poly(ADP-ribose) polymerase 1 (PARP-1), can be considered as a potential anticancer drug candidate. Here we describe a study of 7-MG inhibition mechanism using molecular dynamics, fluorescence anisotropy and single-particle Forster resonance energy transfer (spFRET) microscopy approaches to elucidate intermolecular interactions between 7-MG, PARP-1 and nucleosomal DNA. It is shown that 7-MG competes with substrate NAD+ an...
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#1Olga I. Lavrik (RAS: Russian Academy of Sciences)H-Index: 25
Source
#1Inna A. Vasil'eva (RAS: Russian Academy of Sciences)H-Index: 8
#2N. A. Moor (RAS: Russian Academy of Sciences)H-Index: 10
Last. Olga I. Lavrik (RAS: Russian Academy of Sciences)H-Index: 25
view all 3 authors...
Base excision repair (BER) ensures correction of most abundant DNA lesions in mammals. The efficiency of this multistep DNA repair process that can occur via different pathways depends on the coordinated action of enzymes catalyzing its individual steps. The scaffold XRCC1 (X-ray repair cross-complementing protein 1) protein plays an important coordinating role in the repair of damaged bases and apurinic/apyrimidinic (AP) sites via short-patch (SP) BER pathway, as well as in the repair of single...
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#1Nadejda I. Rechkunova (NSU: Novosibirsk State University)H-Index: 14
#2Olga I. Lavrik (NSU: Novosibirsk State University)H-Index: 25
Replication protein A (RPA), eukaryotic single-stranded DNA-binding protein, is a key player in multiple processes of DNA metabolism including DNA replication, recombination and DNA repair. Human RPA composed of subunits of 70-, 32- and 14-kDa binds ssDNA with high affinity and interacts specifically with multiple proteins. The RPA heterotrimer binds ssDNA in several modes, with occlusion lengths of 8-10, 13-22 and 30 nucleotides corresponding to global, transitional and elongated conformations ...
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#1M. M. Kutuzov (RAS: Russian Academy of Sciences)H-Index: 8
#2E. A. Belousova (RAS: Russian Academy of Sciences)H-Index: 7
Last. Olga I. Lavrik (RAS: Russian Academy of Sciences)H-Index: 25
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DNA is constantly attacked by different damaging agents; therefore, it requires frequent repair. On the one hand, the base excision repair (BER) system is responsible for the repair of the most frequent DNA lesions. On the other hand, the formation of poly(ADP-ribose) is one of the main DNA damage response reactions that is catalysed by members of the PARP family. PARP1, which belongs to the PARP family and performs approximately 90% of PAR synthesis in cells, could be considered a main regulato...
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#1E.M. Mamontova (NSU: Novosibirsk State University)
#2A. L. Zakharenko (RAS: Russian Academy of Sciences)H-Index: 10
Last. Olga I. Lavrik (RAS: Russian Academy of Sciences)H-Index: 25
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Abstract Spinocerebellar ataxia syndrome with axonal neuropathy (SCAN1) is a debilitating neurological disease that is caused by the mutation the Tyrosyl-DNA phosphodiesterase 1 (TDP1) DNA repair enzyme. The crucial His493 in TDP1’s binding site is replaced with an arginine amino acid residue rendering the enzyme dysfunctional. A virtual screen was performed against the homology model of SCAN1 and seventeen compounds were identified and tested in a novel SCAN1 specific biochemical assay. Six com...
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#1N. A. Moor (RAS: Russian Academy of Sciences)H-Index: 10
#2Inna A. Vasil'eva (RAS: Russian Academy of Sciences)H-Index: 8
Last. Olga I. Lavrik (RAS: Russian Academy of Sciences)H-Index: 25
view all 4 authors...
Apurinic/apyrimidinic endonuclease 1 (APE1) is an essential multifunctional protein in mammals involved in base excision DNA repair (BER), regulation of gene expression and RNA metabolism. Its major enzymatic function is incision of AP sites. Poly(ADP-ribose) polymerase 1 (PARP1) modifies itself and target proteins with poly(ADP-ribose) (PAR), contributing to regulation of many processes. To understand molecular basis of functional cooperation between APE1 and PARP1 in BER, we examined PAR-bindi...
1 CitationsSource
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