Yukihito Ishizaka
Molecular biologyVirologyImmunologyCancer researchBiology
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Publications 113
Immune checkpoint blockade (ICB) therapy improved the prognosis of cancer patients, but general administration of ICBs occasionally induces side effects that include immune-related adverse events and tumour hyper-progression. Here, we established a protein-based system, by which endogenous expression of IC molecule in natural killer (NK) cells was transiently repressed on enhancement of their antitumour activity. A protein-based genome modulator (GM) system is composed of a transcription activat...
Abstract We developed a detergent-free enzyme-linked immunosorbent assay (ELISA) for HIV-1 viral protein R (Vpr), an accessory protein of human immunodeficiency virus type-1 (HIV), and detected sol...
1 CitationsSource
#1Kenta IijimaH-Index: 8
#2Junya Kobayashi (Kyoto University)H-Index: 28
Last. Yukihito IshizakaH-Index: 30
view all 3 authors...
Viral protein R (Vpr) is an accessory protein of HIV-1, which is potentially involved in the infection of macrophages and the induction of the ataxia-telangiectasia and Rad3-related protein (ATR)-mediated DNA damage response (DDR). It was recently proposed that the SLX4 complex of structure-specific endonuclease is involved in Vpr-induced DDR, which implies that aberrant DNA structures are responsible for this phenomenon. However, the mechanism by which Vpr alters the DNA structures remains uncl...
4 CitationsSource
#1Tomoki TakashinaH-Index: 1
#2Takayoshi KoyamaH-Index: 6
Last. Yukihito IshizakaH-Index: 30
view all 12 authors...
Abstract Cellular reprogramming is a promising technology in regenerative medicine, but most studies have been performed by using expression vectors. For future clinical applications, it is necessary to establish a system in which cell engineering can be manipulated without any risk of damaging the genome. Here, we identified a cell-penetrating peptide composed of 10 amino acids (RIFIHFRIGC) with nuclear trafficking activity and found that it was significantly more potent than a Tat-derived pept...
3 CitationsSource
#1Tomoki TakashinaH-Index: 1
#2Yukihito IshizakaH-Index: 30
Direct reprogramming is a promising technology in regenerative medicine. However, there is no report on the clinical applications of cells prepared by direct reprogramming. In the current review, we describe direct reprogramming methods of somatic cells to hepatocytes, pancreatic ?-cells, cardiomyocytes, and endothelial cells. Next, we discuss current issues that should be clarified for their future clinical applications. As the most critical issue, it is necessary to establish a vector-free sys...
#2Akira IshiguroH-Index: 1
Last. Yukihito IshizakaH-Index: 30
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#1Mari ShimuraH-Index: 20
#2Hideo ShindouH-Index: 23
Last. Takao Shimizu (UTokyo: University of Tokyo)H-Index: 82
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Fatty acids are taken up by cells and incorporated into complex lipids such as neutral lipids and glycerophospholipids. Glycerophospholipids are major constituents of cellular membranes. More than ...
5 CitationsSource
#1Akihiro MatsunagaH-Index: 5
#2Lui YoshidaH-Index: 1
Last. Mari ShimuraH-Index: 20
view all 8 authors...