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Maria Jasin
Memorial Sloan Kettering Cancer Center
216Publications
76H-index
20.4kCitations
Publications 216
Newest
#1Edwige B. Garcin (AMU: Aix-Marseille University)H-Index: 4
#2Stéphanie Gon (AMU: Aix-Marseille University)H-Index: 3
Last.Kara A. Bernstein (University of Pittsburgh)H-Index: 17
view all 0 authors...
Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells and in transformed U2OS and HEK293 cells. In U2OS and HEK293 cells, viable ablated clones were readily isolated for each RAD51 paralog; in contrast, with the exception of RAD51B, RAD51 paralogs are cell...
#1Edwige B. Garcin (AMU: Aix-Marseille University)H-Index: 4
#2Stéphanie Gon (AMU: Aix-Marseille University)H-Index: 3
Last.Sonja Eberth (Leibniz Association)H-Index: 3
view all 13 authors...
Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells and in transformed U2OS and HEK293 cells. In U2OS and HEK293 cells, viable ablated clones were readily isolated for each RAD51 paralog; in contrast, with the exception of RAD51B, RAD51 paralogs are cell...
#1Wei-Feng YenH-Index: 4
#2Rahul SharmaH-Index: 1
Last.William T. YewdellH-Index: 5
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Summary The immunoglobulin heavy chain ( Igh ) locus features a dynamic chromatin landscape to promote class switch recombination (CSR), yet the mechanisms that regulate this landscape remain poorly understood. CHD4, a component of the chromatin remodeling NuRD complex, directly binds H3K9me3, an epigenetic mark present at the Igh locus during CSR. We find that CHD4 is essential for early B cell development but is dispensable for the homeostatic maintenance of mature, naive B cells. However, los...
#1Robert A. Baldock (University of Pittsburgh)H-Index: 5
#2Catherine A. Pressimone (University of Pittsburgh)H-Index: 1
Last.Rohit Prakash (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 4
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Abstract The proficiency of cancer cells to repair DNA double-strand breaks (DSBs) by homologous recombination (HR) is a key determinant in predicting response to targeted therapies such as PARP inhibitors. The RAD51 paralogs work as multimeric complexes and act downstream of BRCA1 to facilitate HR. Numerous epidemiological studies have linked RAD51 paralog mutations with hereditary cancer predisposition. Despite their substantial links to cancer, RAD51 paralog HR function has remained elusive. ...
#1Laurent Acquaviva (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 2
#2Michiel Boekhout (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 2
Last.Scott Keeney (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 47
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Sex chromosomes in males share only a diminutive homologous segment, the pseudoautosomal region (PAR), wherein meiotic double-strand breaks (DSBs), pairing, and crossing over must occur for correct segregation. How cells ensure PAR recombination is unknown. Here we delineate cis- and trans-acting factors that control PAR ultrastructure and make the PAR the hottest area of DSB formation in the male mouse genome. Prior to DSB formation, PAR chromosome axes elongate, sister chromatids separate, and...
#1Carla M. Abreu (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 2
#2Rohit Prakash (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 4
Last.Maria Jasin (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 76
view all 6 authors...
The DNA-damage repair pathway homologous recombination (HR) requires factors that promote the activity of strand-exchange protein RAD51 and its meiosis-specific homolog DMC1. Here we show that the Shu complex SWS1-SWSAP1, a candidate for one such HR regulator, is dispensable for mouse viability but essential for male and female fertility, promoting the assembly of RAD51 and DMC1 on early meiotic HR intermediates. Only a fraction of mutant meiocytes progress to form crossovers, which are crucial ...
#1Weiran Feng (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 4
#2Maria Jasin (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 76
BRCA2 deficiency causes genome instability and breast and ovarian cancer predisposition, but also paradoxically promotes cell lethality. The nature of the acute, detrimental consequences of BRCA2 loss is not fully understood. We recently generated BRCA2 conditional models from a non-transformed human mammary cell line, through allele-specific gene targeting using CRISPR-Cas9, which we now describe. With these models, we discovered that BRCA2 deficiency triggers a DNA under replication-53BP1 nucl...
#1Chun-Chin ChenH-Index: 4
#2Weiran FengH-Index: 4
Last.Maria JasinH-Index: 11
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Germ-line and somatic mutations in genes that promote homology-directed repair (HDR), especially BRCA1 and BRCA2, are frequently observed in several cancers, in particular, breast and ovary but also prostate and other cancers. HDR is critical for the error-free repair of DNA double-strand breaks and other lesions, and HDR factors also protect stalled replication forks. As a result, loss of BRCA1 or BRCA2 poses significant risks to genome integrity, leading not only to cancer predisposition but a...
#1Erika Brunet (Paris V: Paris Descartes University)
#2Maria Jasin (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 76
Chromosomal translocations are associated with several tumor types, including hematopoietic malignancies, sarcomas, and solid tumors of epithelial origin, due to their activation of a proto-oncogene or generation of a novel fusion protein with oncogenic potential. In many cases, the availability of suitable human models has been lacking because of the difficulty in recapitulating precise expression of the fusion protein or other reasons. Further, understanding how translocations form mechanistic...
#1Carla M. Abreu (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 2
#2Rohit Prakash (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 4
Last.Maria Jasin (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 76
view all 6 authors...
Homology recognition and DNA-strand invasion ensure faithful homolog pairing and segregation during the first meiotic division. RAD51 and DMC1 recombinases catalyze these steps, with BRCA2 promoting their assembly into nuclear foci. The recently identified human SWS1-SWSAP1 complex, related to the Shu complex in yeast, promotes RAD51 focus formation in cell lines. We show here that mouse SWS1-SWSAP1 is critical for meiotic homologous recombination (HR) by promoting the assembly of RAD51 and DMC1...
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