Katarzyna M. Sitnik
Technical University of Denmark
Molecular biologyMesenchymal stem cellImmunologyRetinoic acidBiology
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Publications 14
#1Andrea Cossarizza (University of Modena and Reggio Emilia)H-Index: 67
#2Hyun-Dong ChangH-Index: 25
Last. Arturo Zychlinsky (MPG: Max Planck Society)H-Index: 45
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These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, feat...
74 CitationsSource
#1Nico A. Contreras (UA: University of Arizona)H-Index: 2
Last. Janko Nikolich-Žugich (UA: University of Arizona)H-Index: 20
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Cytomegalovirus (CMV) is a ubiquitous herpesvirus infecting most of the world’s population. CMV has been rigorously investigated for its impact on lifelong immunity and potential complications arising from lifelong infection. A rigorous adaptive immune response mounts during progression of CMV infection from acute to latent states. CD8 T cells, in large part, drive this response and have very clearly been demonstrated to take up residence in the salivary gland and lungs of infected mice during l...
#1Kerstin Wendland (Lund University)H-Index: 1
#2Kristoffer Niss (Novo Nordisk Foundation)H-Index: 1
Last. William W. Agace (DTU: Technical University of Denmark)H-Index: 49
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Despite the essential role of thymic epithelial cells (TEC) in T cell development, the signals regulating TEC differentiation and homeostasis remain incompletely understood. In this study, we show a key in vivo role for the vitamin A metabolite, retinoic acid (RA), in TEC homeostasis. In the absence of RA signaling in TEC, cortical TEC (cTEC) and CD80 lo MHC class II lo medullary TEC displayed subset-specific alterations in gene expression, which in cTEC included genes involved in epithelial pro...
1 CitationsSource
#1Nicole Claudine von Burg (DTU: Technical University of Denmark)
Last. William W. Agace (DTU: Technical University of Denmark)H-Index: 49
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Last. William W. AgaceH-Index: 49
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#1Lisa BorknerH-Index: 3
Last. Luka Cicin-SainH-Index: 21
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Experimental CMV-based vaccine vectors expressing a single MHC class I–restricted high-avidity epitope provided strong, T cell–dependent protection against viruses or tumors. In this study we tested the low-avidity epitope KCSRNRQYL, and show that a mouse CMV (MCMV) vector provides complete immune control of recombinant vaccinia virus expressing the same epitope if KCSRNRQYL is expressed within the immediate-early MCMV gene ie2 . The same epitope expressed within the early M45 gene provided no p...
13 CitationsSource
#1Kerstin Wendland (Lund University)H-Index: 1
Last. William W. Agace (National Veterinary Institute)H-Index: 49
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#1K. Luda (Lund University)H-Index: 3
#2Thorsten Joeris (DTU: Technical University of Denmark)H-Index: 13
Last. William W. Agace (DTU: Technical University of Denmark)H-Index: 49
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Summary The role of dendritic cells (DCs) in intestinal immune homeostasis remains incompletely defined. Here we show that mice lacking IRF8 transcription-factor-dependent DCs had reduced numbers of T cells in the small intestine (SI), but not large intestine (LI), including an almost complete absence of SI CD8αβ + and CD4 + CD8αα + T cells; the latter requiring β8 integrin expression by migratory IRF8 dependent CD103 + CD11b - DCs. SI homing receptor induction was impaired during T cell priming...
54 CitationsSource
#1Katarzyna M. Sitnik (DTU: Technical University of Denmark)H-Index: 7
#2Kerstin Wendland (Lund University)H-Index: 1
Last. William W. Agace (DTU: Technical University of Denmark)H-Index: 49
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Despite the key role of primary and secondary lymphoid organ stroma in immunity, our understanding of the heterogeneity and ontogeny of these cells remains limited. Here, we identify a functionally distinct subset of BP3(-)PDPN(+)PDGFRβ(+)/α(+)CD34(+) stromal adventitial cells in both lymph nodes (LNs) and thymus that is located within the vascular niche surrounding PDPN(-)PDGFRβ(+)/α(-)Esam-1(+)ITGA7(+) pericytes. CD34(+) adventitial cells developed in late embryonic thymus and in postnatal LNs...
16 CitationsSource
#1Beth Lucas (University of Birmingham)H-Index: 7
#2Andrea J. White (University of Birmingham)H-Index: 21
Last. Antal Rot (Ebor: University of York)H-Index: 45
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Thymus colonisation and thymocyte positioning are regulated by interactions between CCR7 and CCR9, and their respective ligands, CCL19/CCL21 and CCL25. The ligands of CCR7 and CCR9 also interact with the atypical receptor CCRL1 (also known as ACKR4), which is expressed in the thymus and has recently been reported to play an important role in normal alpha beta T-cell development. Here, we show that CCRL1 is expressed within the thymic cortex, predominantly by MHC-II(low)CD40(-) cortical thymic ep...
11 CitationsSource