Match!
Greg J. Nusz
Duke University
10Publications
6H-index
516Citations
Publications 10
Newest
1 CitationsSource
Gold nanoparticles provide a template for preparing supported lipid layers with well-defined curvature. Here, we utilize the localized surface plasmon resonance (LSPR) of gold nanoparticles as a sensor for monitoring the preparation of lipid layers on nanoparticles. The LSPR is very sensitive to the immediate surroundings of the nanoparticle surface, and it is used to monitor the coating of lipids and subsequent conversion of a supported bilayer to a hybrid membrane with an outer lipid leaflet a...
23 CitationsSource
#1Greg J. Nusz (Duke University)H-Index: 6
#2Ashutosh Chilkoti (Duke University)H-Index: 80
This chapter reviews our work and that of other groups in the use of individual plasmonic nanostructures that are presented by a substrate for the label-free detection of biomolecular binding events. This class of single particle nanosensors is based on the local surface plasmon resonance (LSPR) behavior of noble metal nanostructures that enables optical transduction of binding events at their surface into an optical signal [1–5]. The LSPR peak location and intensity are sensitive to the local r...
1 CitationsSource
#1Greg J. Nusz (Duke University)H-Index: 6
#2Stella M. Marinakos (Duke University)H-Index: 15
Last.Ashutosh Chilkoti (Duke University)H-Index: 80
view all 4 authors...
The development of truly scalable, multiplexed optical microarrays requires a detection platform capable of simultaneous detection of multiple signals in real-time. We present a technique we term dual-order snapshot spectroscopic imaging (DOSSI) and demonstrate that it can be effectively used to collect spectrally resolved images of a full field of view of sparsely located spots in real time. Resonant peaks of plasmonic gold nanoparticles were tracked as a function of their surrounding refractiv...
8 CitationsSource
#1Greg J. NuszH-Index: 6
#2Adam C. CurryH-Index: 7
Last.Ashutosh Chilkoti (Duke University)H-Index: 80
view all 5 authors...
We present the development of an analytical model that can be used for the rational design of a biosensor based on shifts in the local surface plasmon resonance (LSPR) of individual gold nanoparticles. The model relates the peak wavelength of light scattered by an individual plasmonic nanoparticle to the number of bound analyte molecules and provides an analytical formulation that predicts relevant figures-of-merit of the sensor such as the molecular detection limit (MDL) and dynamic range as a ...
155 CitationsSource
#1Greg J. NuszH-Index: 6
#2Stella M. MarinakosH-Index: 15
Last.Ashutosh ChilkotiH-Index: 80
view all 7 authors...
We report the use of individual gold nanorods as plasmonic transducers to detect the binding of streptavidin to individual biotin-conjugated nanorods in real time on a surface. Label-free detection at the single-nanorod level was performed by tracking the wavelength shift of the nanorod-localized surface plasmon resonant scattering spectrum using a dark-field microspectroscopy system. The lowest streptavidin concentration that was experimentally measured was 1 nM, which is a factor of 1000-fold ...
215 CitationsSource
#1Adam C. Curry (Duke University)H-Index: 7
#2Greg J. Nusz (Duke University)H-Index: 6
Last.Adam Wax (Duke University)H-Index: 40
view all 4 authors...
One goal of recent research on plasmonic nanoparticle-based sensors is maximizing nanoparticle sensitivity or shift of resonance peak wavelength per refractive index change. Equally important is a measurement system's peak location uncertainty or shift resolution. We provide systematic analyses and discuss optimization of factors that determine peak location uncertainty, reporting values as low as 0.3 nm for the presented scheme. This type of analysis is important, in part, because it provides a...
16 CitationsSource
#1Greg J. Nusz (NCSU: North Carolina State University)H-Index: 6
#2Eva Johannes (NCSU: North Carolina State University)H-Index: 20
Last.Hans D. Hallen (NCSU: North Carolina State University)H-Index: 21
view all 4 authors...
Probes designed to locally illuminate structures within plant cells are described. The probes studied are etch-sharpened single mode optical fibers, coated with aluminum, similar to probes used for near-field scanning optical microscopy. We find that cellular material adheres to the probes that are not coated with a self-assembled monolayer octadecyltrichlorosilane. The hydrophobic monolayer coating enabled these probes to be inserted into and removed from plant cells with no protein adhesion to...
3 CitationsSource
#1Adam C. Curry (Duke University)H-Index: 7
#2Greg J. Nusz (Duke University)H-Index: 6
Last.Adam Wax (Duke University)H-Index: 40
view all 4 authors...
We use optical darkfield micro-spectroscopy to characterize the plasmon resonance of individual silver nanoparticles in the presence of a substrate. The optical system permits multiple individual nanoparticles to be identified visually for simultaneous spectroscopic study. For silver particles bound to a silanated glass substrate, we observe changes in the plasmon resonance due to induced variations in the local refractive index. The shifts in the plasmon resonance are investigated using a simpl...
94 CitationsSource
1