Jason Hammonds
Cincinnati Children's Hospital Medical Center
Molecular biologyVirologyTetherinPseudovirionBiology
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Publications 25
#1Mohammad Ali Rai (UC: University of Cincinnati)H-Index: 1
#2Jason Hammonds (Cincinnati Children's Hospital Medical Center)H-Index: 15
Last. Paul Spearman (Cincinnati Children's Hospital Medical Center)H-Index: 38
view all 3 authors...
#1Junghwa Kirschman (Emory University)H-Index: 1
#2Mingli Qi (Emory University)H-Index: 6
Last. Paul Spearman (Cincinnati Children's Hospital Medical Center)H-Index: 38
view all 9 authors...
8 CitationsSource
#1Jason Hammonds (Cincinnati Children's Hospital Medical Center)H-Index: 15
#2Neal Beeman (Emory University)H-Index: 8
Last. Paul Spearman (Cincinnati Children's Hospital Medical Center)H-Index: 38
view all 8 authors...
HIV-1 particles assemble and bud from the plasma membrane of infected T lymphocytes. Infected macrophages, in contrast, accumulate particles within an apparent intracellular compartment known as the virus-containing compartment or VCC. Many aspects of the formation and function of the VCC remain unclear. Here we demonstrate that VCC formation does not actually require infection of the macrophage, but can be reproduced through the exogenous addition of non-infectious virus-like particles or infec...
25 CitationsSource
#1Cheri M. HamptonH-Index: 9
#2Joshua D. StraussH-Index: 6
Last. Elizabeth R. WrightH-Index: 25
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Correlative light and electron microscopy (CLEM) promises new insight into cellular processes by combining spatiotemporal information with high-resolution structural information. This protocol describes cryo-CLEM of virus-infected mammalian cells.
40 CitationsSource
#1Michael G. Currier (Emory University)H-Index: 8
#2Sujin Lee (Emory University)H-Index: 17
Last. Martin L. Moore (Emory University)H-Index: 28
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Respiratory syncytial virus (RSV) is the major cause of viral lower respiratory tract illness in children. In contrast to the RSV prototypic strain A2, clinical isolate RSV 2–20 induces airway mucin expression in mice, a clinically relevant phenotype dependent on the fusion (F) protein of the RSV strain. Epidermal growth factor receptor (EGFR) plays a role in airway mucin expression in other systems; therefore, we hypothesized that the RSV 2–20 F protein stimulates EGFR signaling. Infection of c...
19 CitationsSource
#1Joshua D. Strauss (Emory University)H-Index: 6
#2Jason Hammonds (Emory University)H-Index: 15
Last. Elizabeth R. Wright (Emory University)H-Index: 25
view all 6 authors...
ABSTRACT Tetherin (BST2, CD317, or HM1.24) is a host cellular restriction factor that prevents the release of enveloped viruses by mechanically linking virions to the plasma membrane. The precise arrangement of tetherin molecules at the plasma membrane site of HIV-1 assembly, budding, and restriction is not well understood. To gain insight into the biophysical mechanism underlying tetherin-mediated restriction of HIV-1, we utilized cryo-electron tomography (cryo-ET) to directly visualize HIV-1 v...
17 CitationsSource
#1Charline Giroud (Emory University)H-Index: 3
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory B. Melikyan (Emory University)H-Index: 23
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HIV-1 Env glycoprotein-mediated fusion is initiated upon sequential binding of Env to CD4 and the coreceptor CXCR4 or CCR5. Whereas these interactions are thought to be necessary and sufficient to promote HIV-1 fusion, other host factors can modulate this process. Previous studies reported potent inhibition of HIV-1 fusion by selective P2X1 receptor antagonists, including NF279, and suggested that these receptors play a role in HIV-1 entry. Here we investigated the mechanism of antiviral activit...
7 CitationsSource
#1Mingli Qi (Emory University)H-Index: 6
#2Hin Chu (HKU: University of Hong Kong)H-Index: 18
Last. Paul Spearman (Emory University)H-Index: 38
view all 9 authors...
Lentiviruses such as HIV-1 encode envelope glycoproteins (Env) with long cytoplasmic tails (CTs) that include motifs mediating interactions with host-cell–trafficking factors. We demonstrated recently that Rab11-family interacting protein 1C (FIP1C) is required for CT-dependent incorporation of Env into HIV-1 particles. Here, we used viruses bearing targeted substitutions within CT to map the FIP1C-dependent incorporation of Env. We identified YW795 as a critical motif mediating cell-type–depend...
16 CitationsSource
#1Hong Yi (Emory University)H-Index: 19
#2Joshua D. StraussH-Index: 6
Last. Elizabeth R. WrightH-Index: 25
view all 11 authors...
Numerous methods have been developed for immunogold labeling of thick, cryo-preserved biological specimens. However, most of the methods are permutations of chemical fixation and sample sectioning, which select and isolate the immunolabeled region of interest. We describe a method for combining immunogold labeling with cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) of the surface proteins of intact mammalian cells or the surface glycoproteins of assembling and budding ...
12 CitationsSource
#1Hong Yi (Emory University)H-Index: 19
#2Joshua D. Strauss (Emory University)H-Index: 6
Last. Elizabeth R. Wright (Emory University)H-Index: 25
view all 7 authors...