(CAS: Chinese Academy of Sciences)+ 14 AuthorsNing Yao2
Estimated H-index: 2
(SJTU: Shanghai Jiao Tong University)
Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
Monogenic disorders occur at a high frequency in human populations and are commonly inherited through the germline. Unfortunately, once the mutation has been transmitted to a child, only limited treatment options are available in most cases. However, means of correcting disease-causing nuclear and mitochondrial DNA mutations in gametes or preimplantation embryos have now been developed and are commonly referred to as germline gene therapy (GGT). We will discuss these novel strategies and provide...
(Cardiovascular Institute of the South)+ 1 AuthorsSanjiv Kaul81
Estimated H-index: 81
(Cardiovascular Institute of the South)
Summary A variety of genetic cardiovascular diseases may one day be curable using gene editing technology. Germline genome editing and correction promises to permanently remove monogenic cardiovascular disorders from the offspring and subsequent generations of affected families. Although technically feasible and likely to be ready for implementation in humans in the near future, this approach remains ethically controversial. Although currently beset by several technical challenges, and not yet p...
(Oregon National Primate Research Center)+ 2 AuthorsFuhua Xu1
Estimated H-index: 1
(OHSU: Oregon Health & Science University)
Objective To study whether follicular growth and oocyte maturation can be improved by antimullerian hormone (AMH) modulation at specific stages of follicular development. Design Primary and secondary follicles were cultured in a matrix-free system and were assigned to the control group and the group with AMH supplementation during the preantral stage and neutralizing AMH antibody addition during the antral stage. Setting National primate research center. Animal(s) Adult, female rhesus macaques (...
(Oregon National Primate Research Center), Yeonmi Lee6
Estimated H-index: 6
(Oregon National Primate Research Center)+ 12 AuthorsHayley Darby1
Estimated H-index: 1
(Oregon National Primate Research Center)
The accumulation of acquired mitochondrial genome (mtDNA) mutations with aging in somatic cells has been implicated in mitochondrial dysfunction and linked to age-onset diseases in humans. Here, we asked if somatic mtDNA mutations are also associated with aging in the mouse. MtDNA integrity in multiple organs and tissues in young and old (2–34 months) wild type (wt) mice was investigated by whole genome sequencing. Remarkably, no acquired somatic mutations were detected in tested tissues. Howeve...
Abstract Patient-specific pluripotent stem cells (PSCs) can be generated via nuclear reprogramming by transcription factors (i.e., induced pluripotent stem cells, iPSCs) or by somatic cell nuclear transfer (SCNT). However, abnormalities and preclinical application of differentiated cells generated by different reprogramming mechanisms have yet to be evaluated. Here we investigated the molecular and functional features, and drug response of cardiomyocytes (PSC-CMs) and endothelial cells (PSC-ECs)...