Shoukhrat Mitalipov
Oregon Health & Science University
Embryonic stem cellSomatic cell nuclear transferGeneticsBiologyCell biology
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Publications 129
#1Shoukhrat Mitalipov (OHSU: Oregon Health & Science University)H-Index: 38
Applications of genome editing ultimately depend on DNA repair triggered by targeted double-strand breaks (DSBs). However, repair mechanisms in human cells remain poorly understood and vary across different cell types. Here we report that DSBs selectively induced on a mutant allele in heterozygous human embryos are repaired by gene conversion using an intact wildtype homolog as a template in up to 40% of targeted embryos. We also show that targeting of homozygous loci facilitates an interplay of...
2 CitationsSource
#1Seongjun So (UOU: University of Ulsan)H-Index: 1
#2Yeon-Mi LeeH-Index: 11
Last. Eunju KangH-Index: 14
view all 14 authors...
: Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative and is cheaper than Y-27632. It increased hPSC growth following thawing and passaging, like Y-27...
3 CitationsSource
#1Hong Ma (Oregon National Primate Research Center)H-Index: 18
#2Tomonari Hayama (OHSU: Oregon Health & Science University)H-Index: 8
Last. Shoukhrat Mitalipov (Oregon National Primate Research Center)H-Index: 38
view all 21 authors...
Heritable mitochondrial DNA (mtDNA) mutations are common, yet only a few recurring pathogenic mtDNA variants account for the majority of known familial cases in humans. Purifying selection in the female germline is thought to be responsible for the elimination of most harmful mtDNA mutations during oogenesis. Here we show that deleterious mtDNA mutations are abundant in ovulated mature mouse oocytes and preimplantation embryos recovered from PolG mutator females but not in their live offspring. ...
1 CitationsSource
#1Eunju Kang (UOU: University of Ulsan)H-Index: 14
#1Eunju Kang (UOU: University of Ulsan)
Last. Shoukhrat Mitalipov (OHSU: Oregon Health & Science University)H-Index: 38
view all 5 authors...
#1Meiling Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 6
#2Changyang Zhou (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Hui Yang (CAS: Chinese Academy of Sciences)H-Index: 19
view all 17 authors...
Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be ...
3 CitationsSource
#1Eunju Kang (Oregon National Primate Research Center)H-Index: 14
#2Jun Wu (Salk Institute for Biological Studies)H-Index: 33
Last. Shoukhrat MitalipovH-Index: 38
view all 30 authors...
Change history In this Letter, there are several errors regarding the assignments of mtDNA haplotypes for a subset of egg donors from our study. These errors have not been corrected online.
2 CitationsSource
#1David German (OHSU: Oregon Health & Science University)H-Index: 3
#2Shoukhrat Mitalipov (Cardiovascular Institute of the South)H-Index: 38
Last. Sanjiv Kaul (OHSU: Oregon Health & Science University)H-Index: 80
view all 4 authors...
Summary A variety of genetic cardiovascular diseases may one day be curable using gene editing technology. Germline genome editing and correction promises to permanently remove monogenic cardiovascular disorders from the offspring and subsequent generations of affected families. Although technically feasible and likely to be ready for implementation in humans in the near future, this approach remains ethically controversial. Although currently beset by several technical challenges, and not yet p...
8 CitationsSource
#1Don P. Wolf (OHSU: Oregon Health & Science University)H-Index: 42
#2Paul A. Mitalipov (OHSU: Oregon Health & Science University)H-Index: 1
Last. Shoukhrat Mitalipov (OHSU: Oregon Health & Science University)H-Index: 38
view all 3 authors...
Monogenic disorders occur at a high frequency in human populations and are commonly inherited through the germline. Unfortunately, once the mutation has been transmitted to a child, only limited treatment options are available in most cases. However, means of correcting disease-causing nuclear and mitochondrial DNA mutations in gametes or preimplantation embryos have now been developed and are commonly referred to as germline gene therapy (GGT). We will discuss these novel strategies and provide...
4 CitationsSource
#1Jing Xu (Oregon National Primate Research Center)H-Index: 13
#2Maralee S. Lawson (Oregon National Primate Research Center)H-Index: 9
Last. Fuhua Xu (OHSU: Oregon Health & Science University)H-Index: 2
view all 5 authors...
Objective To study whether follicular growth and oocyte maturation can be improved by antimullerian hormone (AMH) modulation at specific stages of follicular development. Design Primary and secondary follicles were cultured in a matrix-free system and were assigned to the control group and the group with AMH supplementation during the preantral stage and neutralizing AMH antibody addition during the antral stage. Setting National primate research center. Animal(s) Adult, female rhesus macaques (...
2 CitationsSource
#1Hong MaH-Index: 18
Last. Shoukhrat MitalipovH-Index: 38
view all 30 authors...