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Jacob E. Corn
ETH Zurich
98Publications
32H-index
4,606Citations
Publications 95
Newest
#1Rutger David Luteijn (University of California, Berkeley)H-Index: 2
#2Shivam A. Zaver (UW: University of Washington)H-Index: 2
Last.David H. Raulet (University of California, Berkeley)H-Index: 84
view all 11 authors...
The accumulation of DNA in the cytosol serves as a key immunostimulatory signal associated with infections, cancer and genomic damage1,2. Cytosolic DNA triggers immune responses by activating the cyclic GMP–AMP synthase (cGAS)–stimulator of interferon genes (STING) pathway3. The binding of DNA to cGAS activates its enzymatic activity, leading to the synthesis of a second messenger, cyclic guanosine monophosphate–adenosine monophosphate (2′3′-cGAMP)4–7. This cyclic dinucleotide (CDN) activates ST...
4 CitationsSource
#1Alan S Wang (University of California, Berkeley)
#2Leo Chen (University of California, Berkeley)
Last.Jacob E. Corn (ETH Zurich)H-Index: 32
view all 11 authors...
Summary Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Using a proximity labeling system for unbiased detection of transient protein interactions in cell-free Xenopus laevis egg extract, we identified the dimeric histone chaperone FACT as an interactor of substrate-bound Ca...
Source
#1Shaheen Kabir (University of California, Berkeley)H-Index: 1
#2Justin Cidado (AstraZeneca)H-Index: 10
Last.Jacob E. Corn (University of California, Berkeley)H-Index: 32
view all 11 authors...
Organisms keep their tissues healthy by instructing damaged or unwanted cells to kill themselves via a controlled process known as apoptosis. Cancer cells, however, are able to evade death by increasing the level of proteins that block apoptosis, such as MCL1. Researchers have recently developed new drugs that can inhibit the action of the MCL1 protein. But a number of cancers have become resistant to these inhibitors. So, one important question is whether other proteins in cancer cells could be...
Source
#1Mark C. WaltersH-Index: 36
#2Wendy MagisH-Index: 3
Last.D. MartínH-Index: 1
view all 11 authors...
Source
#1Beeke Wienert (University of California, Berkeley)H-Index: 8
#2Stacia K. Wyman (University of California, Berkeley)H-Index: 4
Last.Jacob E. Corn (University of California, Berkeley)H-Index: 32
view all 14 authors...
CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target sites. Current off-target discovery methods work using purified DNA or specific cellular models but are incapable of direct detection in vivo. We developed DISCOVER-Seq (discovery of in situ Cas off-targets and verification by sequencing), a universally applicable approach for unbiased off-target identification that leverages the recruitment of DNA repair factors in cells and organisms. Tracking the precise recr...
13 CitationsSource
#1John C. Rose (UW: University of Washington)H-Index: 3
#2Nicholas A. Popp (UW: University of Washington)
Last.Douglas M. Fowler (CIFAR: Canadian Institute for Advanced Research)H-Index: 22
view all 9 authors...
Abstract CRISPR/Cas9 nucleases are powerful genome engineering tools, but unwanted cleavage at off-target and previously edited sites remains a major concern. Numerous strategies to reduce unwanted cleavage have been devised, but all are imperfect. Here, we report off-target sites can be shielded from the active Cas9•single guide RNA (sgRNA) complex through the co-administration of dead-RNAs (dRNAs), truncated guide RNAs that direct Cas9 binding but not cleavage. dRNAs can effectively suppress a...
Source
#1Jin Rui Liang (ETH Zurich)H-Index: 1
#2Emily Lingeman (University of California, Berkeley)H-Index: 2
Last.Jacob E. Corn (ETH Zurich)H-Index: 32
view all 7 authors...
Selective degradation of organelles via autophagy is critical for cellular differentiation, homeostasis, and organismal health. Autophagy of the ER (ER-phagy) is implicated in human neuropathy but is poorly understood beyond a few specialized autophagosomal receptors and remodelers. Using an ER-phagy reporter and genome-wide CRISPRi screening, we identified 200 high-confidence factors involved in human ER-phagy. We mechanistically investigated two pathways unexpectedly required for ER-phagy. Fir...
Source
#1Rutger David Luteijn (University of California, Berkeley)H-Index: 2
#2Shivam A. Zaver (UW: University of Washington)H-Index: 2
Last.David H. RauletH-Index: 84
view all 11 authors...
The accumulation of DNA in the cytosol serves as a key immunostimulatory signal associated with infections, cancer and genomic damage. Cytosolic DNA triggers immune responses by activating the cGAS/STING pathway. The binding of DNA to the cytosolic enzyme cGAMP synthase (cGAS), activates its enzymatic activity, leading to the synthesis of a second messenger, cyclic[G(29,59)pA(3959)] (2939-cGAMP). 2939-cGAMP, a cyclic dinucleotide (CDN), activates the protein 9stimulator of interferon genes9 (STI...
2 CitationsSource
#1Anastasia Lomova (UCLA: University of California, Los Angeles)H-Index: 4
#2Danielle N. Clark (UCLA: University of California, Los Angeles)H-Index: 1
Last.Donald B. Kohn (UCLA: University of California, Los Angeles)H-Index: 71
view all 14 authors...
7 CitationsSource
#1Jennifer E. Chung (University of California, Berkeley)H-Index: 1
#2Wendy Magis (Children's Hospital Oakland Research Institute)H-Index: 6
Last.Mark A. DeWitt (University of California, Berkeley)H-Index: 10
view all 12 authors...
Author(s): Chung, Jennifer E; Magis, Wendy; Vu, Jonathan; Heo, Seok-Jin; Wartiovaara, Kirmo; Walters, Mark C; Kurita, Ryo; Nakamura, Yukio; Boffelli, Dario; Martin, David I. K; Corn, Jacob E; DeWitt, Mark A | Abstract: Sickle Cell Disease and s-thalassemia, which are caused by defective or deficient adult s-globin (HBB) respectively, are the most common serious genetic blood diseases in the world. Persistent expression of the fetal s-like globin, also known as ?-globin, can ameliorate both disor...
4 CitationsSource
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