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Jacob E. Corn
University of California, Berkeley
82Publications
29H-index
3,893Citations
Publications 82
Newest
Published on Apr 3, 2019in bioRxiv
John C. Rose3
Estimated H-index: 3
(UW: University of Washington),
Nicholas A Popp (UW: University of Washington)+ 6 AuthorsDouglas M. Fowler19
Estimated H-index: 19
(UW: University of Washington)
Abstract CRISPR/Cas9 nucleases are powerful genome engineering tools, but unwanted cleavage at off-target and previously edited sites remains a major concern. Numerous strategies to reduce unwanted cleavage have been devised, but all are imperfect. Here, we report off-target sites can be shielded from the active Cas9•single guide RNA (sgRNA) complex through the co-administration of dead-RNAs (dRNAs), truncated guide RNAs that direct Cas9 binding but not cleavage. dRNAs can effectively suppress a...
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Published on Feb 25, 2019in bioRxiv
Jin Rui Liang1
Estimated H-index: 1
(ETH Zurich),
Emily Lingeman2
Estimated H-index: 2
(University of California, Berkeley)
+ 4 AuthorsJacob E. Corn29
Estimated H-index: 29
(ETH Zurich)
Selective degradation of organelles via autophagy is critical for cellular differentiation, homeostasis, and organismal health. Autophagy of the ER (ER-phagy) is implicated in human neuropathy but is poorly understood beyond a few specialized autophagosomal receptors and remodelers. Using an ER-phagy reporter and genome-wide CRISPRi screening, we identified 200 high-confidence factors involved in human ER-phagy. We mechanistically investigated two pathways unexpectedly required for ER-phagy. Fir...
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Published on Feb 4, 2019in bioRxiv
Rutger Luteijn (University of California, Berkeley), Shivam Zaver (UW: University of Washington)+ 8 AuthorsJoshua J. Woodward18
Estimated H-index: 18
(UW: University of Washington)
The accumulation of DNA in the cytosol serves as a key immunostimulatory signal associated with infections, cancer and genomic damage. Cytosolic DNA triggers immune responses by activating the cGAS/STING pathway. The binding of DNA to the cytosolic enzyme cGAMP synthase (cGAS), activates its enzymatic activity, leading to the synthesis of a second messenger, cyclic[G(29,59)pA(3959)] (2939-cGAMP). 2939-cGAMP, a cyclic dinucleotide (CDN), activates the protein 9stimulator of interferon genes9 (STI...
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Published on Feb 1, 2019in Stem Cells 5.59
Anastasia Lomova3
Estimated H-index: 3
(UCLA: University of California, Los Angeles),
Danielle N. Clark1
Estimated H-index: 1
(UCLA: University of California, Los Angeles)
+ 11 AuthorsMark A. DeWitt10
Estimated H-index: 10
(University of California, Berkeley)
2 Citations Source Cite
Published on Jan 11, 2019in bioRxiv
Jacob E. Corn29
Estimated H-index: 29
(ETH Zurich),
Shaheen Kabir (UCSF: University of California, San Francisco)+ 8 AuthorsMatthew Belmonte4
Estimated H-index: 4
(AstraZeneca)
Overexpression of anti-apoptotic proteins MCL1 and Bcl-xL is a frequent event in blood and solid cancers. Inhibitors targeting MCL1 are in clinical development, however many cancer models are intrinsically resistant to this approach. To discover mechanisms underlying resistance to MCL1 inhibition, we performed multiple flow-cytometry based genome-wide CRISPR screens that interrogate two drugs directly or indirectly targeting MCL1. Remarkably, both screens identified three components (CUL5, RNF7 ...
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Published on Dec 19, 2018in bioRxiv
Beeke Wienert6
Estimated H-index: 6
(Gladstone Institutes),
Sharon J. Feng1
Estimated H-index: 1
(University of California, Berkeley)
+ 6 AuthorsJacob E. Corn29
Estimated H-index: 29
(University of California, Berkeley)
Repair of double strand DNA breaks (DSBs) can result in gene disruption or precise gene modification via homology directed repair (HDR) from a templating donor DNA. During genome editing, altering cellular responses to DSBs may be an effective strategy to rebalance editing outcomes towards HDR and away from other repair pathways. To identify factors that regulate HDR from a double-stranded DNA donor (dsDonor), we utilized a pooled screen to define the consequences of thousands of individual gene...
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Published on Nov 5, 2018in Journal of Cell Biology 8.78
Jin Rui Liang1
Estimated H-index: 1
,
Emily Lingeman2
Estimated H-index: 2
+ 1 AuthorsJacob E. Corn29
Estimated H-index: 29
Vol. 217, No. 10, October 1, 2018. [10.1083/jcb.201804185][1]. The immunofluorescence image in [Figure 2][2] I was mislabeled as GFP-LC3B but is in fact immunofluorescence staining of endogenous LC3B. This information was correctly described in the original main text and figure legend and therefore
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Published on Oct 3, 2018in bioRxiv
Wendy Magis6
Estimated H-index: 6
(Children's Hospital Oakland Research Institute),
Mark A. DeWitt10
Estimated H-index: 10
(University of California, Berkeley)
+ 17 AuthorsMatthew S. McNeill2
Estimated H-index: 2
(Integrated DNA Technologies)
Sickle Cell Disease (SCD), one of the world9s most common genetic disorders, causes anemia and progressive multiorgan damage that typically shortens lifespan by decades; currently there is no broadly applicable curative therapy. Here we show that Cas9 RNP-mediated gene editing with an ssDNA oligonucleotide donor yields more than 20% correction of the sickle mutation in long-term engrafting human HSCs. Using RNA-seq, we further find that in vivo erythroid differentiation markedly enriches for cel...
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