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Jacob E. Corn
University of California, Berkeley
90Publications
29H-index
3,894Citations
Publications 90
Newest
Published on Sep 1, 2019in Nature43.07
Rutger Luteijn (University of California, Berkeley), Shivam Zaver (UW: University of Washington)+ 8 AuthorsJoshua J. Woodward18
Estimated H-index: 18
(UW: University of Washington)
The accumulation of DNA in the cytosol serves as a key immunostimulatory signal associated with infections, cancer and genomic damage1,2. Cytosolic DNA triggers immune responses by activating the cyclic GMP–AMP synthase (cGAS)–stimulator of interferon genes (STING) pathway3. The binding of DNA to cGAS activates its enzymatic activity, leading to the synthesis of a second messenger, cyclic guanosine monophosphate–adenosine monophosphate (2′3′-cGAMP)4–7. This cyclic dinucleotide (CDN) activates ST...
Published on Jul 23, 2019in bioRxiv
Alan S Wang (University of California, Berkeley), Leo Chen (University of California, Berkeley)+ 8 AuthorsJohannes C. Walter49
Estimated H-index: 49
(Harvard University)
Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Using a proximity labeling system for unbiased detection of transient protein interactions in cell-free Xenopus laevis egg extract, we identified the dimeric histone chaperone FACT as an interactor of substrate-bound Cas9. Immu...
Published on Jul 11, 2019in eLife7.55
Shaheen Kabir (University of California, Berkeley), Justin Cidado8
Estimated H-index: 8
(AstraZeneca)
+ 8 AuthorsLisa Drew7
Estimated H-index: 7
(AstraZeneca)
Published on Apr 3, 2019in bioRxiv
John C. Rose3
Estimated H-index: 3
(UW: University of Washington),
Nicholas A Popp (UW: University of Washington)+ 6 AuthorsDouglas M. Fowler20
Estimated H-index: 20
(UW: University of Washington)
Abstract CRISPR/Cas9 nucleases are powerful genome engineering tools, but unwanted cleavage at off-target and previously edited sites remains a major concern. Numerous strategies to reduce unwanted cleavage have been devised, but all are imperfect. Here, we report off-target sites can be shielded from the active Cas9•single guide RNA (sgRNA) complex through the co-administration of dead-RNAs (dRNAs), truncated guide RNAs that direct Cas9 binding but not cleavage. dRNAs can effectively suppress a...
Published on Feb 25, 2019in bioRxiv
Jin Rui Liang1
Estimated H-index: 1
(ETH Zurich),
Emily Lingeman2
Estimated H-index: 2
(University of California, Berkeley)
+ 4 AuthorsJacob E. Corn29
Estimated H-index: 29
(ETH Zurich)
Selective degradation of organelles via autophagy is critical for cellular differentiation, homeostasis, and organismal health. Autophagy of the ER (ER-phagy) is implicated in human neuropathy but is poorly understood beyond a few specialized autophagosomal receptors and remodelers. Using an ER-phagy reporter and genome-wide CRISPRi screening, we identified 200 high-confidence factors involved in human ER-phagy. We mechanistically investigated two pathways unexpectedly required for ER-phagy. Fir...
Published on Feb 4, 2019in bioRxiv
Rutger Luteijn (University of California, Berkeley), Shivam Zaver (UW: University of Washington)+ 8 AuthorsJacob E. Corn29
Estimated H-index: 29
(University of California, Berkeley)
The accumulation of DNA in the cytosol serves as a key immunostimulatory signal associated with infections, cancer and genomic damage. Cytosolic DNA triggers immune responses by activating the cGAS/STING pathway. The binding of DNA to the cytosolic enzyme cGAMP synthase (cGAS), activates its enzymatic activity, leading to the synthesis of a second messenger, cyclic[G(29,59)pA(3959)] (2939-cGAMP). 2939-cGAMP, a cyclic dinucleotide (CDN), activates the protein 9stimulator of interferon genes9 (STI...
Published on Feb 1, 2019in Stem Cells5.61
Anastasia Lomova3
Estimated H-index: 3
(UCLA: University of California, Los Angeles),
Danielle N. Clark1
Estimated H-index: 1
(UCLA: University of California, Los Angeles)
+ 11 AuthorsMark A. DeWitt10
Estimated H-index: 10
(University of California, Berkeley)
Published on 2019in PLOS ONE2.78
Jennifer E Chung1
Estimated H-index: 1
,
Wendy Magis6
Estimated H-index: 6
+ 7 AuthorsDavid I. K. Martin43
Estimated H-index: 43
Author(s): Chung, Jennifer E; Magis, Wendy; Vu, Jonathan; Heo, Seok-Jin; Wartiovaara, Kirmo; Walters, Mark C; Kurita, Ryo; Nakamura, Yukio; Boffelli, Dario; Martin, David I. K; Corn, Jacob E; DeWitt, Mark A | Abstract: Sickle Cell Disease and s-thalassemia, which are caused by defective or deficient adult s-globin (HBB) respectively, are the most common serious genetic blood diseases in the world. Persistent expression of the fetal s-like globin, also known as ?-globin, can ameliorate both disor...
Published on Jan 11, 2019in bioRxiv
Jacob E. Corn29
Estimated H-index: 29
(ETH Zurich),
Shaheen Kabir (UCSF: University of California, San Francisco)+ 8 AuthorsMatthew Belmonte4
Estimated H-index: 4
(AstraZeneca)
Overexpression of anti-apoptotic proteins MCL1 and Bcl-xL is a frequent event in blood and solid cancers. Inhibitors targeting MCL1 are in clinical development, however many cancer models are intrinsically resistant to this approach. To discover mechanisms underlying resistance to MCL1 inhibition, we performed multiple flow-cytometry based genome-wide CRISPR screens that interrogate two drugs directly or indirectly targeting MCL1. Remarkably, both screens identified three components (CUL5, RNF7 ...
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