Match!
Aaron M. Kerlin
Howard Hughes Medical Institute
13Publications
10H-index
1,663Citations
Publications 14
Newest
#1Aaron M. Kerlin (HHMI: Howard Hughes Medical Institute)H-Index: 10
#2Mohar Boaz (HHMI: Howard Hughes Medical Institute)H-Index: 1
Last.Karel Svoboda (HHMI: Howard Hughes Medical Institute)H-Index: 97
view all 8 authors...
The active properties of dendrites can support local nonlinear operations, but previous imaging and electrophysiological measurements have produced conflicting views regarding the prevalence and selectivity of local nonlinearities in vivo. We imaged calcium signals in pyramidal cell dendrites in the motor cortex of mice performing a tactile decision task. A custom microscope allowed us to image the soma and up to 300 μm of contiguous dendrite at 15 Hz, while resolving individual spines. New anal...
1 CitationsSource
Source
#1Hod Dana (Cleveland Clinic)H-Index: 14
#2Yi Sun (HHMI: Howard Hughes Medical Institute)H-Index: 14
Last.Douglas S. Kim (HHMI: Howard Hughes Medical Institute)H-Index: 23
view all 18 authors...
Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely used to measure neural activity in intact nervous systems. GECIs are frequently used in one of two different modes: to track activity in large populations of neuronal cell bodies, or to follow dynamics in subcellular compartments such as axons, dendrites and individual synaptic compartments. Despite major advances, calcium imaging is still limited by the biophysical properties of existing GECIs, including affinity, ...
24 CitationsSource
#1Aaron M. Kerlin (HHMI: Howard Hughes Medical Institute)H-Index: 10
#2Boaz Mohar (HHMI: Howard Hughes Medical Institute)H-Index: 10
Last.Karel Svoboda (HHMI: Howard Hughes Medical Institute)H-Index: 97
view all 7 authors...
The active properties of dendrites support local nonlinear operations, but previous imaging and electrophysiological measurements have produced conflicting views regarding the prevalence of local nonlinearities in vivo. We imaged calcium signals in pyramidal cell dendrites in the motor cortex of mice performing a tactile decision task. A custom microscope allowed us to image the soma and up to 300 μm of contiguous dendrite at 15 Hz, while resolving individual spines. New analysis methods were us...
6 CitationsSource
#1Rongwen Lu (HHMI: Howard Hughes Medical Institute)H-Index: 12
#2Wenzhi Sun (HHMI: Howard Hughes Medical Institute)H-Index: 16
Last.Na Ji (HHMI: Howard Hughes Medical Institute)H-Index: 22
view all 14 authors...
The authors built a simple optical module that generates axially extended Bessel foci, optimized for in vivo brain imaging. Easily incorporated into existing two-photon fluorescence microscopes, this module allowed 30-Hz volumetric functional imaging of sparsely labeled brains at synaptic resolution in a variety of model organisms in vivo.
82 CitationsSource
#1Glenn J. GoldeyH-Index: 5
#2Demetris K RoumisH-Index: 5
Last.Mark L. AndermannH-Index: 26
view all 8 authors...
This protocol describes how to implant a removable cranial window, enabling manipulations such as targeted virus injections and microprism insertion, for one- and two-photon calcium imaging of the same cortical brain regions for several months.
110 CitationsSource
#1Chen WangH-Index: 13
#2Rui LiuH-Index: 2
Last.Na JiH-Index: 22
view all 9 authors...
An adaptive optics method using multiplexed light measurement and modulation in multiple pupil segments improves structural and functional in vivo imaging over large volumes in strongly scattering mouse brain with only a single aberration correction.
69 CitationsSource
#1Rui Liu (HHMI: Howard Hughes Medical Institute)H-Index: 2
#2Daniel E. MilkieH-Index: 20
Last.Na Ji (HHMI: Howard Hughes Medical Institute)H-Index: 22
view all 5 authors...
In traditional zonal wavefront sensing for adaptive optics, after local wavefront gradients are obtained, the entire wavefront can be calculated by assuming that the wavefront is a continuous surface. Such an approach will lead to sub-optimal performance in reconstructing wavefronts which are either discontinuous or undersampled by the zonal wavefront sensor. Here, we report a new method to reconstruct the wavefront by directly measuring local wavefront phases in parallel using multidither coher...
14 CitationsSource
#1Mark L. Andermann (Harvard University)H-Index: 26
#2Aaron M. Kerlin (Harvard University)H-Index: 10
Last.R. Clay Reid (Harvard University)H-Index: 46
view all 5 authors...
Summary The mouse is emerging as an important model for understanding how sensory neocortex extracts cues to guide behavior, yet little is known about how these cues are processed beyond primary cortical areas. Here, we used two-photon calcium imaging in awake mice to compare visual responses in primary visual cortex (V1) and in two downstream target areas, AL and PM. Neighboring V1 neurons had diverse stimulus preferences spanning five octaves in spatial and temporal frequency. By contrast, AL ...
223 CitationsSource
#1Davi Bock (Harvard University)H-Index: 11
#2Wei-Chung Allen Lee (Harvard University)H-Index: 13
Last.R. Clay Reid (Harvard University)H-Index: 46
view all 10 authors...
To date, various aspects of connectivity have been inferred from electron microscopy (EM) of synaptic contacts, light microscopy of axonal and dendritic arbors, and correlations in activity. However, until now it has not been possible to relate the complex structural wiring between neurons to the function of individual cells. Using a combination of functional imaging and three-dimensional serial EM reconstruction at unprecedented scale, two papers now describe the connectivity of single cells in...
575 CitationsSource
12