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Aaron M. Kerlin
Howard Hughes Medical Institute
NeuroscienceCalcium imagingNeuronVisual cortexBiology
15Publications
9H-index
1,955Citations
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Publications 15
Newest
#1Aaron M. Kerlin (HHMI: Howard Hughes Medical Institute)H-Index: 9
#2Mohar Boaz (HHMI: Howard Hughes Medical Institute)H-Index: 1
Last. Karel Svoboda (HHMI: Howard Hughes Medical Institute)H-Index: 104
view all 8 authors...
The active properties of dendrites can support local nonlinear operations, but previous imaging and electrophysiological measurements have produced conflicting views regarding the prevalence and selectivity of local nonlinearities in vivo. We imaged calcium signals in pyramidal cell dendrites in the motor cortex of mice performing a tactile decision task. A custom microscope allowed us to image the soma and up to 300 μm of contiguous dendrite at 15 Hz, while resolving individual spines. New anal...
6 CitationsSource
#1Aaron M. Kerlin (HHMI: Howard Hughes Medical Institute)H-Index: 9
#2Mohar Boaz (HHMI: Howard Hughes Medical Institute)H-Index: 1
Last. Karel Svoboda (HHMI: Howard Hughes Medical Institute)H-Index: 104
view all 8 authors...
Source
#1Hod Dana (Cleveland Clinic)H-Index: 15
#2Yi Sun (HHMI: Howard Hughes Medical Institute)H-Index: 14
Last. Douglas S. Kim (HHMI: Howard Hughes Medical Institute)H-Index: 22
view all 18 authors...
Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely used to measure neural activity in intact nervous systems. GECIs are frequently used in one of two different modes: to track activity in large populations of neuronal cell bodies, or to follow dynamics in subcellular compartments such as axons, dendrites and individual synaptic compartments. Despite major advances, calcium imaging is still limited by the biophysical properties of existing GECIs, including affinity, ...
88 CitationsSource
#1Aaron M. Kerlin (HHMI: Howard Hughes Medical Institute)H-Index: 9
#2Boaz Mohar (HHMI: Howard Hughes Medical Institute)H-Index: 9
Last. Karel Svoboda (HHMI: Howard Hughes Medical Institute)H-Index: 104
view all 7 authors...
The active properties of dendrites support local nonlinear operations, but previous imaging and electrophysiological measurements have produced conflicting views regarding the prevalence of local nonlinearities in vivo. We imaged calcium signals in pyramidal cell dendrites in the motor cortex of mice performing a tactile decision task. A custom microscope allowed us to image the soma and up to 300 μm of contiguous dendrite at 15 Hz, while resolving individual spines. New analysis methods were us...
8 CitationsSource
#1Rongwen Lu (HHMI: Howard Hughes Medical Institute)H-Index: 13
#2Wenzhi Sun (HHMI: Howard Hughes Medical Institute)H-Index: 16
Last. Na Ji (HHMI: Howard Hughes Medical Institute)H-Index: 23
view all 14 authors...
The authors built a simple optical module that generates axially extended Bessel foci, optimized for in vivo brain imaging. Easily incorporated into existing two-photon fluorescence microscopes, this module allowed 30-Hz volumetric functional imaging of sparsely labeled brains at synaptic resolution in a variety of model organisms in vivo.
82 CitationsSource
#1Rongwen Lu (HHMI: Howard Hughes Medical Institute)H-Index: 13
#2Wenzhi Sun (HHMI: Howard Hughes Medical Institute)H-Index: 16
Last. Na Ji (HHMI: Howard Hughes Medical Institute)H-Index: 23
view all 14 authors...
Neurons and neural networks often extend hundreds to thousands of micrometers in three dimensions. To capture all the calcium transients associated with their activity, we need volume imaging methods with sub-second temporal resolution. Such speed is challenging for conventional two-photon laser scanning microscopy (2PLSM) to achieve, because of its dependence on serial focal scanning in 3D and the limited brightness of indicators. Here we present an optical module that can be easily integrated ...
6 CitationsSource
#1Glenn J. Goldey (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 5
#2Demetris K. Roumis (UCSF: University of California, San Francisco)H-Index: 5
Last. Mark L. Andermann (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 27
view all 8 authors...
This protocol describes how to implant a removable cranial window, enabling manipulations such as targeted virus injections and microprism insertion, for one- and two-photon calcium imaging of the same cortical brain regions for several months.
149 CitationsSource
#1Chen Wang (HHMI: Howard Hughes Medical Institute)H-Index: 14
#2Rui Liu (HHMI: Howard Hughes Medical Institute)H-Index: 2
Last. Na Ji (HHMI: Howard Hughes Medical Institute)H-Index: 23
view all 9 authors...
An adaptive optics method using multiplexed light measurement and modulation in multiple pupil segments improves structural and functional in vivo imaging over large volumes in strongly scattering mouse brain with only a single aberration correction.
76 CitationsSource
#1Rui Liu (HHMI: Howard Hughes Medical Institute)H-Index: 2
#2Daniel E. MilkieH-Index: 20
Last. Na Ji (HHMI: Howard Hughes Medical Institute)H-Index: 23
view all 5 authors...
In traditional zonal wavefront sensing for adaptive optics, after local wavefront gradients are obtained, the entire wavefront can be calculated by assuming that the wavefront is a continuous surface. Such an approach will lead to sub-optimal performance in reconstructing wavefronts which are either discontinuous or undersampled by the zonal wavefront sensor. Here, we report a new method to reconstruct the wavefront by directly measuring local wavefront phases in parallel using multidither coher...
15 CitationsSource
#1Mark L. Andermann (Harvard University)H-Index: 27
#2Aaron M. Kerlin (Harvard University)H-Index: 9
Last. R. Clay Reid (Harvard University)H-Index: 48
view all 5 authors...
Summary The mouse is emerging as an important model for understanding how sensory neocortex extracts cues to guide behavior, yet little is known about how these cues are processed beyond primary cortical areas. Here, we used two-photon calcium imaging in awake mice to compare visual responses in primary visual cortex (V1) and in two downstream target areas, AL and PM. Neighboring V1 neurons had diverse stimulus preferences spanning five octaves in spatial and temporal frequency. By contrast, AL ...
257 CitationsSource
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