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Niraja Dighe
National University of Singapore
15Publications
5H-index
279Citations
Publications 15
Newest
#1Niraja Dighe (NUS: National University of Singapore)H-Index: 5
#2Kang Wei Tan (NUS: National University of Singapore)
Last.Citra Nurfarah Zaini Mattar (NUS: National University of Singapore)H-Index: 13
view all 14 authors...
Major hemoglobinopathies place tremendous strain on global resources. Intrauterine hemopoietic cell transplantation (IUHCT) and gene transfer (IUGT) can potentially reduce perinatal morbidities with greater efficacy than postnatal therapy alone. We performed both procedures in the thalassemic HbbTh3/+ mouse. Intraperitoneal delivery of co-isogenic cells at embryonic days13-14 produced dose-dependent chimerism. High-dose adult bone marrow (BM) cells maintained 0.2–3.1% chimerism over ~24 weeks an...
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#1Jerry K. Y. Chan (NUS: National University of Singapore)H-Index: 35
#2Lay-Geok Tan (NUS: National University of Singapore)H-Index: 4
Last.Citra Nurfarah Zaini Mattar (NUS: National University of Singapore)H-Index: 13
view all 7 authors...
Intrauterine haemopoietic stem cell therapy (IUHSCT) can be considered for treating haemoglobinopathies and certain metabolic diseases in early pregnancy, for the ability to easily escalate cell dose for higher engraftment and to target the pre-immune fetus. The potential therapeutic benefits are currently unrealised due to sub-therapeutic cell engraftment from host cell competition for haemopoietic niches or neutralising immune response.We investigated the strategy of IUHSCT followed by post-na...
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#1Niraja Dighe (NUS: National University of Singapore)H-Index: 5
#2Maroun Khoury (MIT: Massachusetts Institute of Technology)H-Index: 4
Last.Jerry Kok Yen Chan (NUS: National University of Singapore)H-Index: 6
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Hematopoietic Stem Cell (HSC) targeted gene transfer is an attractive treatment option for a number of hematopoietic disorders caused by single gene defects. However, extensive methylation of promoter sequences results in silencing of therapeutic gene expression. The choice of an appropriate promoter is therefore crucial for reproducible, stable and long-term transgene expression in clinical gene therapy. Recent studies suggest efficient and stable expression of transgenes from the ubiquitous ch...
12 CitationsSource
#1Ali NowrouziH-Index: 17
#2Wing T. Cheung (Imperial College London)H-Index: 2
Last.Michael Themis (Imperial College London)H-Index: 25
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Genotoxicity models are extremely important to assess retroviral vector biosafety before gene therapy. We have developed an in utero model that demonstrates that hepatocellular carcinoma (HCC) development is restricted to mice receiving nonprimate (np) lentiviral vectors (LV) and does not occur when a primate (p) LV is used regardless of woodchuck post-translation regulatory element (WPRE) mutations to prevent truncated X gene expression. Analysis of 839 npLV and 244 pLV integrations in the live...
15 CitationsSource
#1Citra Nurfarah Zaini Mattar (NUS: National University of Singapore)H-Index: 13
#2Amit C. Nathwani (UCL: University College London)H-Index: 41
Last.Jerry K. Y. Chan (NUS: National University of Singapore)H-Index: 35
view all 20 authors...
Intrauterine gene transfer (IUGT) offers ontological advantages including immune naivete mediating tolerance to the vector and transgenic products, and effecting a cure before development of irreversible pathology. Despite proof-of-principle in rodent models, expression efficacy with a therapeutic transgene has yet to be demonstrated in a preclinical nonhuman primate (NHP) model. We aimed to determine the efficacy of human Factor IX (hFIX) expression after adeno-associated-viral (AAV)-mediated I...
40 CitationsSource
#2Amit C. NathwaniH-Index: 41
Last.Jerry K. Y. ChanH-Index: 35
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#2Niraja DigheH-Index: 5
Last.Jerry K. Y. ChanH-Index: 35
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2 Citations
#1Michael ThemisH-Index: 25
#2Simon N. WaddingtonH-Index: 40
Last.Charles CoutelleH-Index: 41
view all 19 authors...
34 Citations
#1Simon N. Waddington (Imperial College London)H-Index: 40
#2Manfred Schmidt (University of Freiburg)H-Index: 76
Last.Charles Coutelle (Imperial College London)H-Index: 41
view all 20 authors...
1 CitationsSource
#1Michael Themis (Imperial College London)H-Index: 25
#2Simon N. Waddington (Imperial College London)H-Index: 40
Last.Charles Coutelle (Imperial College London)H-Index: 41
view all 20 authors...
The authors regret that in Table 2 on page 768, one of the insertion sites of the SMART 2 provirus vector identified using LAM-PCR as present on chromosome 5 positioned 32374 bp upstream of Cyp3a11 was incorrectly assigned to Mouse (tumour) 2 T1. This insertion site should be assigned to an independent mouse not listed in Table 2. This animal had only a single provirus insertion found by Southern and LAM-PCR analyses and should be labeled as mouse 7.
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