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Stanley Koszelak
University of California, Riverside
19Publications
12H-index
505Citations
Publications 19
Newest
The protein thaumatin was studied as a model macromolecule for crystallization in microgravity-environment experiments conducted on two US Space Shuttle missions (USML-2 and LMS). In this investigation, we have evaluated and compared the quality of space- and earth-grown thaumatin crystals using X-ray diffraction analyses, and characterized them according to crystal size, diffraction resolution limit and mosaicity. Two different approaches for growing thaumatin crystals in the microgravity envir...
#1Stanley KoszelakH-Index: 12
#2Joseph D. NgH-Index: 13
Last.Alexander McPhersonH-Index: 53
view all 6 authors...
The major extracellular protease from the fungus Pencillium cyclopium was crystallized in the presence of p-phenylmethanesulfonyl fluoride (PMSF) and investigated by X-ray diffraction analysis. It was subsequently cloned and the amino acid sequence deduced from its cDNA. Although the sequence is only 49% identical to that of proteinase K of Tritirachium album, the three-dimensional structures of the two proteases are virtually identical. The model for P. cyclopium protease was refined by simulat...
#1Stanley Koszelak (UCR: University of California, Riverside)H-Index: 12
#2Cathy Leja (UCR: University of California, Riverside)H-Index: 1
Last.Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
view all 3 authors...
One hundred eighty-three flash frozen, liquid–liquid diffusion and batch method protein and virus crystallization samples were launched aboard the Space Shuttle Discovery on June 27 (STS-71) and transferred to the Russian Space Station Mir on July 1, 1995. They were returned to earth November 20, 1995 (STS-74). Subsequent examination showed that of the 19 types of proteins and viruses investigated, 17 were crystallized during the period on Mir. The experiment demonstrates the utility of this ver...
#1Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
#2A Malkin (UCR: University of California, Riverside)H-Index: 18
Last.Stanley Koszelak (UCR: University of California, Riverside)H-Index: 12
view all 4 authors...
The chemical, mechanical and diffraction properties of crystals grown from solution, as well as their growth kinetics and morphological development, depend very much on the types and concentrations of impurities present in their mother liquor. The situation appears vastly more complicated in the case of macromolecular crystals because of the complex nature of the molecules and the biochemical milieu from which they are derived. An attempt is made here to catalog and characterize these various im...
#1Stanley Koszelak (UCR: University of California, Riverside)H-Index: 12
#2John Day (UCR: University of California, Riverside)H-Index: 15
Last.Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
view all 5 authors...
Two T = 1 and one T = 3 plant viruses, along with a protein, were crystallized in microgravity during the International Microgravity Laboratory-2 (IML-2) mission in July of 1994. The method used was liquid-liquid diffusion in the European Space Agency's Advanced Protein Crystallization Facility (APCF). Distinctive alterations in the habits of Turnip Yellow Mosaic Virus (TYMV) crystals and hexagonal canavalin crystals were observed. Crystals of cubic Satellite Tobacco Mosaic Virus (STMV) more tha...
#1Steven B. Larson (UCR: University of California, Riverside)H-Index: 13
#2Stanley Koszelak (UCR: University of California, Riverside)H-Index: 12
Last.Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
view all 6 authors...
Abstract The crystal structure of satellite tobacco mosaic virus (STMV) has been solved by a combination of multiple isomorphous replacement and molecular replacement methods and refined at 2·9 A resolution to a conventional R -factor of 0·215. SMTV, a T = 1 icosahedral virus, is the smallest whose structure has been determined. The coat protein is an eight-stranded "Swiss roll" β-barrel with an amino-terminal strand that extends away from the β-barrel by more than 60 A. This strand is primarily...
#1Steven B. Larson (UCR: University of California, Riverside)H-Index: 13
#2Stanley Koszelak (UCR: University of California, Riverside)H-Index: 12
Last.Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
view all 6 authors...
SATELLITE tobacco mosaic virus (STMV) is the spherical satellite to an obligatory rod-shaped helper tobacco mosaic virus (TMV), which is required for replication1,2. STMV has 60 protein subunits of Mr 17,500 on a T = 1 icosahedral capsid3 containing a single stranded RNA genome of 1,059 bases4–6. STMV appears similar to another virus, STNV7,8, but is ~20 per cent smaller. It shows no amino-acid homology or immunological cross-reactivity with either STNV or its host TMV4,9. Here we report the X-r...
#1Lawrence J. DeLucas (UAB: University of Alabama at Birmingham)H-Index: 24
#2Craig D. Smith (UAB: University of Alabama at Birmingham)H-Index: 14
Last.L.L. Clancy (Upjohn)H-Index: 5
view all 10 authors...
Results obtained from the Shuttle flight STS-32 flown in January 1990, and preliminary results from the most recent Shuttle flight, STS-31, flown in April 1990, are presented. Crystals grown in microgravity environment include Canavalin, isocitrate lyase, human serum albumin, and Anti-HPr Fab. It is concluded that about 20 percent of proteins flown exhibit better morphologies or better quality data than their earth-grown counterparts. About 40 percent do not yield crystals at all and the remaini...
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