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Howard A. Nash
Laboratory of Molecular Biology
71Publications
34H-index
4,841Citations
Publications 71
Newest
Dongyu Guo2
Estimated H-index: 2
(Laboratory of Molecular Biology),
Thomas S. Dexheimer24
Estimated H-index: 24
(National Institutes of Health),
Yves Pommier97
Estimated H-index: 97
(National Institutes of Health)
... more
Abstract Tyrosyl-DNA phosphodiesterase (TDP1) is a phylogenetically conserved enzyme critical for the removal of blocking lesions at the 3′ ends of DNA or RNA. This study analyzes the Drosophila TDP1 gene ortholog glaikit (gkt) and its possible role(s) in the repair of endogenous DNA lesions and neuroprotection. To do so, we studied a homozygous PiggyBac insertion (c03958) that disrupts the 5′ UTR of gkt. Protein extracts of c03958 flies were defective in hydrolyzing 3′-DNA–tyrosyl residues, dem...
Ref 55Cited 9
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2013 in PLOS ONE
Bridget C. Lear11
Estimated H-index: 11
(University of Iowa),
Eric J. Darrah1
Estimated H-index: 1
(University of Iowa),
Benjamin T. Aldrich7
Estimated H-index: 7
(University of Iowa)
... more
In the fruit fly Drosophila melanogaster, a network of circadian pacemaker neurons drives daily rhythms in rest and activity. The ion channel NARROW ABDOMEN (NA), orthologous to the mammalian sodium leak channel NALCN, functions downstream of the molecular circadian clock in pacemaker neurons to promote behavioral rhythmicity. To better understand the function and regulation of the NA channel, we have characterized two putative auxiliary channel subunits in Drosophila, unc79 (aka dunc79) and unc...
Ref 28Cited 17
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2013 in Genes, Brain and Behavior [IF: 3.74]
Elyssa D. Burg9
Estimated H-index: 9
(National Institutes of Health),
Sara T. Langan1
Estimated H-index: 1
(National Institutes of Health),
Howard A. Nash34
Estimated H-index: 34
(National Institutes of Health)
Members of many species tend to congregate, a behavioral strategy known as local enhancement. Selective advantages of local enhancement range from efficient use of resources to defense from predators. While previous studies have examined many types of social behavior in fruit flies, few have specifically investigated local enhancement. Resource-independent local enhancement (RILE) has recently been described in the fruit fly using a measure called social space index (SSI), although the neural me...
Ref 60Cited 9
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2013 in Anesthesiology [IF: 5.79]
Shuying Gao1
Estimated H-index: 1
(University of Maryland, College Park),
David J. Sandstrom1
Estimated H-index: 1
,
Harold E. Smith1
Estimated H-index: 1
... more
Background—Although in vitro studies have identified numerous possible targets, the molecules that mediate the in vivo effects of volatile anesthetics remain largely unknown. The mammalian ryanodine receptor (Ryr) is a known halothane target, and we hypothesized that it has a central role in anesthesia. Methods—Gene function of the Drosophila Ryr (dRyr) was manipulated in the whole body or in specific tissues using a collection of mutants and transgenes, and responses to halothane were measured ...
Ref 42Cited 6
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2009 in Anesthesiology [IF: 5.79]
Debasmita P. Alone1
Estimated H-index: 1
(National Institutes of Health),
Jason C. Rodriguez1
Estimated H-index: 1
,
Cameron L. Noland1
Estimated H-index: 1
... more
Background: Chromosomal deletions and duplications, which result in halving or doubling of copy number in a block of genes, are an important source of variation between individuals. Phenotypic effects of copy number variation are commonly observed, but effects on sensitivity to volatile anesthetics have not been assessed in any organism. Methods: The potency with which halothane depresses the righting reflex of fruit flies was measured in congenic Drosophila strains, each of which was heterozygo...
Ref 42Cited 7
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2009 in Journal of Neurogenetics [IF: 2.29]
Joseph L. Campbell3
Estimated H-index: 3
(Laboratory of Molecular Biology),
Qun Gu1
Estimated H-index: 1
(Laboratory of Molecular Biology),
Dongyu Guo2
Estimated H-index: 2
(Laboratory of Molecular Biology)
... more
Mutations that influence the sensitivity of an organism to a volatile general anesthetic can be divided into two classes. In one, sensitivity to all other volatile agents is affected to a similar degree. Although this class may contain mutations of interest for understanding anesthesia, it is also likely to contain mutations that merely alter general health. In the second class, mutations confer non-uniform effects on potency (NEP), i.e., larger effects for some volatile anesthetics than for oth...
Ref 40Cited 7
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2008 in Genetics [IF: 4.56]
Yuzhong Cheng2
Estimated H-index: 2
(National Institutes of Health),
Howard A. Nash34
Estimated H-index: 34
(National Institutes of Health)
The effect of illumination on alertness can be assessed by comparing the efficacy of an anesthetic under light vs. dark conditions. Results from such tests on wild-type flies and visual mutants demonstrate that, surprisingly, light has both positive and negative influences on arousal. These dual effects may explain aspects of the fly's daily activity and have potential clinical implications.
Ref 24Cited 5
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2007 in Current Biology [IF: 8.85]
John A. Humphrey2
Estimated H-index: 2
,
Kevin S. Hamming3
Estimated H-index: 3
(University of British Columbia),
Colin Thacker9
Estimated H-index: 9
(University of British Columbia)
... more
Summary Volatile anesthetics like halothane and enflurane are of interest to clinicians and neuroscientists because of their ability to preferentially disrupt higher functions that make up the conscious state. All volatiles were once thought to act identically; if so, they should be affected equally by genetic variants. However, mutations in two distinct genes, one in Caenorhabditis and one in Drosophila , have been reported to produce much larger effects on the response to halothane than enflur...
Ref 31Cited 72
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In both vertebrates and invertebrates, ion channels of the TRP superfamily are known to be influenced by a variety of accessory factors, but the list of interacting proteins is acknowledged to be incomplete. Although previous work showed that Drosophila TRP function is disrupted by mutations in the inaF locus, the mechanism of this effect has remained obscure. Here we show that a previously overlooked small protein, INAF-B, is encoded by the locus and fulfills its critical role in retinal physio...
Ref 23Cited 11
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2006 in Genetics [IF: 4.56]
James X. Yu1
Estimated H-index: 1
,
Zhonghui Guan9
Estimated H-index: 9
(Harvard University),
Howard A. Nash34
Estimated H-index: 34
In addition to their well-known effects on the development of the mushroom body, mud mutants are also female sterile. Here we show that, although the early steps of ovary development are grossly normal, a defect becomes apparent in meiosis II when the two component spindles fail to cohere and align properly. The products of meiosis are consequently mispositioned within the egg and, with or without fertilization, soon undergo asynchronous and spatially disorganized replication. In wild-type eggs,...
Ref 37Cited 23
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