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N.S. Laursen
Aarhus University
Complement systemMolecular biologyComplement component 5CrystallographyBiology
30Publications
6H-index
312Citations
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Publications 32
Newest
#1Henrik Pedersen (AU: Aarhus University)H-Index: 25
#2Rasmus K. Jensen (AU: Aarhus University)H-Index: 5
Last. Gregers R. Andersen (AU: Aarhus University)H-Index: 36
view all 7 authors...
The complement system is a tightly controlled proteolytic cascade in the innate immune system, which tags intruding pathogens and dying host cells for clearance. An essential protein in this process is complement component C3. Uncontrolled complement activation has been implicated in several human diseases and disorders and has spurred the development of therapeutic approaches that modulate the complement system. Here, using purified proteins and several biochemical assays and surface plasmon re...
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Chemically modified antigen-binding proteins are widely applied for their targeting abilities in the fields of biotechnol-ogy, medicine and diagnostics. However, the production of site-selectively modified proteins remains a challenge. Here, we have designed a chemical probe for the introduction of a reactive aldehyde on nanobodies by metal complex guided conjugation. The probe design allows for purification of the conjugates and the aldehyde constitutes an efficient handle for further modificat...
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#1Dennis Pedersen (AU: Aarhus University)H-Index: 12
#2Trine A.F. Gadeberg (AU: Aarhus University)H-Index: 2
Last. Gregers R. Andersen (AU: Aarhus University)H-Index: 36
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Properdin (FP) is a positive regulator of the immune system stimulating the activity of the proteolytically active C3 convertase C3bBb in the alternative pathway of the complement system. Here we present two crystal structures of FP and two structures of convertase bound FP. A structural core formed by three thrombospondin repeats (TSRs) and a TB domain harbors the convertase binding site in FP that mainly interacts with C3b. Stabilization of the interaction between the C3b C-terminus and the MI...
2 CitationsSource
#2Dennis PedersenH-Index: 12
Last. Gregers R. AndersenH-Index: 36
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Last. Pontus GourdonH-Index: 16
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P-type ATPases form a large and ubiquitous superfamily of ion and lipid transporters that use ATP (adenosine triphosphate) to carry out their function. The IB subclass (PIB-ATPases) allows flux of heavy metals and are key players in metal detoxification, critical for human health, crops, and survival of pathogens. Nevertheless, PIB-ATPases remain poorly understood at a molecular level. In this study, nanobodies (Nbs) are selected against the zinc-transporting PIB-ATPase ZntA from Shigella sonnei...
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#1N.S. LaursenH-Index: 6
#2Dennis PedersenH-Index: 12
Last. Gregers R. AndersenH-Index: 36
view all 7 authors...
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#1Philip J. R. Roche (McGill University)H-Index: 7
#2Heidi Gytz Olesen (McGill University)
Last. Uri David Akavia (McGill University)H-Index: 7
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The CRISPR/Cas9 system has shown great potential for precisely editing genomic DNA sequences by introducing site-specific DNA cuts that are subsequently repaired by the cell. However, delivery of the CRISPR ribonucleoprotein remains an understudied area and hinders realizing the full potential of the system. We prepared Cas9 ribonucleoprotein complexes chemically conjugated to the 7D12 nanobody and demonstrate receptor-mediated transfection of Cas9 into A549 non-small-cell lung cancer cells via ...
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#1Rasmus K. JensenH-Index: 5
#2Rasmus Pihl (AU: Aarhus University)H-Index: 2
Last. Gregers R. AndersenH-Index: 36
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2 CitationsSource
#1Rasmus K. JensenH-Index: 5
#2Kasper R. AndersenH-Index: 15
Last. Gregers R. AndersenH-Index: 36
view all 5 authors...
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