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Themis J. Michailides
University of California, Davis
BlightBotanyAgronomyFungicideBiology
264Publications
39H-index
5,461Citations
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Publications 260
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#1Hervé F. Avenot (UC Davis: University of California, Davis)H-Index: 8
#2David P. Morgan (UC Davis: University of California, Davis)H-Index: 18
Last. Themis J. Michailides (UC Davis: University of California, Davis)H-Index: 39
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Abstract Botrytis cinerea causes Botrytis blossom, shoot and fruit blight in pistachio and gray mold in grape. The hydroxyanilide (Hyd) fenhexamid, one of the most frequently used fungicides for Botrytis control, inhibits the 3-ketoreductase (Erg27) of the ergosterol biosynthesis pathway. Due to its site-specific mode of action, this fungicide is at-risk for resistance development. In this study, 74 and 58 B. cinerea isolates, collected from Californian pistachio orchards and grape vineyards, re...
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#1Pamela Suellen Salvador Dutra (UFPR: Federal University of Paraná)
#2Paulo S. F. Lichtemberg (UC Davis: University of California, Davis)H-Index: 4
Last. Louise Larissa May De Mio (UFPR: Federal University of Paraná)H-Index: 10
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#1Yong Luo (UC Davis: University of California, Davis)H-Index: 19
#2Franz J. A. Niederholzer (University of California, Berkeley)H-Index: 5
Last. Themis J. Michailides (UC Davis: University of California, Davis)H-Index: 39
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AIMS: A real-time quantitative PCR (qPCR) assay was established to quantify the inoculum densities in the air and rainwater for six canker-causing pathogen groups in prune and walnut orchards in California. METHODS AND RESULTS: The previously published DNA primers to target six pathogen groups including Botryosphaeria dothidea, Cytospora spp., Diplodia spp., Lasiodiplodia spp., Neofusicoccum spp., and Phomopsis spp. were used in a qPCR assay. Air samples from Burkard spore traps and rain samples...
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Aflatoxin contamination of important food and feed crops occurs frequently in warm tropical and subtropical regions. The contamination is caused mainly by Aspergillus flavus and A. parasiticus. Aflatoxin contamination negatively affects health and trade sectors and causes economic losses to agricultural industries. Many pre- and post-harvest technologies can limit aflatoxin contamination but may not always reduce aflatoxin concentrations below tolerance thresholds. However, the use of atoxigenic...
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#1Rong MaH-Index: 2
#2Sheng-Nan LiH-Index: 1
Last. Cheng-Ming TianH-Index: 10
view all 6 authors...
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#1Hervé F. Avenot (UC Davis: University of California, Davis)H-Index: 8
#2David P. Morgan (UC Davis: University of California, Davis)H-Index: 18
Last. Themis J. Michailides (UC Davis: University of California, Davis)H-Index: 39
view all 4 authors...
In this study, a mycelial growth assay was used to evaluate the sensitivity to thiophanate-methyl of 144 Botrytis cinerea isolates (collection A) from Californian vineyards and pistachio and pomegranate orchards. Based on the effective concentration that inhibits 50% of growth (EC50) values for mycelial growth inhibition on fungicide-amended media, 3, 28, 10, and 58% of the isolates showed sensitivity (SS; EC50 100 microg/ml) toward thiophanate-methyl, respectively. The LR and HR phenotypes were...
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#1Hervé F. Avenot (UC Davis: University of California, Davis)H-Index: 8
#2Themis J. Michailides (UC Davis: University of California, Davis)H-Index: 39
: Alternaria late blight (ALB) caused by Alternaria spp. is an annual disease problem in California pistachio and requires repeated applications of fungicides to prevent significant losses of pistachio foliage and nut quality. From 2003 onward, the succinate dehydrogenase inhibiting fungicide boscalid has played a key role in ALB management. The development of boscalid resistance in A. alternata populations was monitored from 2005 to 2012 in pistachio producing areas in California. A total of 1,...
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#1Juan Moral Moral (UC Davis: University of California, Davis)H-Index: 5
#2David P. Morgan (UC Davis: University of California, Davis)H-Index: 18
Last. Themis J. Michailides (UC Davis: University of California, Davis)H-Index: 39
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Abstract Species of Botryosphaeriaceae family cause detrimental damage and yield losses in perennial crops. In California, Botryosphaeriaceae species cause three major diseases, the panicle and shoot blight of pistachio; the canker, dieback, and blight of walnut; and, the band canker of almond. The control of Botryosphaeria diseases of pistachio and walnut by applying synthetic fungicides has been an effective management strategy. Conversely, because the unique disease cycle of almond band canke...
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#1T. A. Carraro (UFPR: Federal University of Paraná)
#2P. S. F. Lichtemberg (UC Davis: University of California, Davis)H-Index: 1
Last. L. L. May-De Mio (UFPR: Federal University of Paraná)
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#1Hervé F. Avenot (UC Davis: University of California, Davis)H-Index: 8
#2Michael Luna (UC Davis: University of California, Davis)H-Index: 1
Last. Themis J. Michailides (UC Davis: University of California, Davis)H-Index: 39
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Abstract In this study, the sensitivity of 902 isolates of A. alternata collected before and after the commercial use of fluopyram were assessed using mycelial growth tests. Distribution of 50% effective concentration (EC50) values for 116 A. alternata isolates (population A), collected in 2011 from an experimental orchard that had been treated with fluopyram products or boscalid showed that 86% of the isolates displayed different levels of resistance to boscalid while 87 (76%) and 28 (24%) show...
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