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Michał Otręba
Medical University of Silesia
MelaninChemistryAntioxidantViability assayBiochemistry
25Publications
9H-index
207Citations
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Publications 25
Newest
#1Michał Otręba (Medical University of Silesia)H-Index: 9
#2Monika Pajor (Medical University of Silesia)
Last. Jared D. Warncke (VCU: Virginia Commonwealth University)H-Index: 1
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Cutaneous melanoma is least common (only about 1% of skin cancers) but is the deadliest malignant tumor. Moreover, amelanotic types of melanoma are very difficult for clinical diagnosis. The standard therapy can cause a lot of side effects, e.g., nausea, vomiting, and headaches, which means that novel and effective strategies are required. Interestingly, phenothiazine derivatives possess sedative, antiemetic, and anticancer activity. Our goal was to determine the effect of perphenazine and proch...
Source
#1Michał Otręba (Medical University of Silesia)H-Index: 9
#2Leon Kośmider (Medical University of Silesia)H-Index: 7
Last. Andrzej Sobczak (Medical University of Silesia)H-Index: 23
view all 5 authors...
3 CitationsSource
#1Michał Otręba (Medical University of Silesia)H-Index: 9
#2Artur Beberok (Medical University of Silesia)H-Index: 9
Last. Ewa Buszman (Medical University of Silesia)H-Index: 18
view all 4 authors...
Purpose Fluphenazine and prochlorperazine as phenothiazine-class antipsychotic drugs are widely used to treat schizophrenia, however their use is associated with significant side effects such as extrapyramidal symptoms, as well as ocular and skin disorders. Our goal was to determine the effect of fluphenazine and prochlorperazine on cell viability and melanogenesis in lightly pigmented normal human melanocytes.
Source
#1Jakub Rok (Medical University of Silesia)H-Index: 7
#2Dorota Wrześniok (Medical University of Silesia)H-Index: 13
Last. Ewa Buszman (Medical University of Silesia)H-Index: 18
view all 6 authors...
Abstract Oxytetracycline is a broad-spectrum antibiotic, used in dermatology and veterinary medicine. Like other tetracyclines, it may evoke skin phototoxic reactions related to generation of reactive oxygen species (ROS). Melanins are biopolymers synthesised in melanocytes – highly specialised cells, localised in the basal layer of epidermis. Production of melanin is a defence mechanism against harmful effects of UV radiation, ROS and many chemical substances, including drugs. In the present st...
2 CitationsSource
#1Marcin Delijewski (Medical University of Silesia)H-Index: 5
#2Dorota Wrześniok (Medical University of Silesia)H-Index: 13
Last. Ewa Buszman (Medical University of Silesia)H-Index: 18
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Abstract Nicotine is a main compound of tobacco plants and may affect more than a billion people all over the world that are permanently exposed to nicotine from cigarettes, various forms of smoking cessation therapies, electronic cigarettes or second-hand smoke. It is known that nicotine forms complexes with melanin what may lead to accumulation of this alkaloid in tissues of living organisms containing the pigment. This may affect the viability of cells and process of melanin biosynthesis that...
3 CitationsSource
#1Jakub Rok (Medical University of Silesia)H-Index: 7
#2Ewa Buszman (Medical University of Silesia)H-Index: 18
Last. Dorota Wrześniok (Medical University of Silesia)H-Index: 13
view all 6 authors...
Doxycycline is a commonly used tetracycline antibiotic showing the broad spectrum of antibacterial action. However, the use of this antibiotic is often connected with the risk of phototoxic reactions that lead to various skin disorders. One of the factors influencing the photosensitivity reactions is the melanin content in melanocytes. In this study, the impact of doxycycline and UVA irradiation on cell viability, melanogenesis and antioxidant defense system in cultured normal human epidermal me...
4 CitationsSource
#1Jakub Rok (Medical University of Silesia)H-Index: 7
#2Ewa Buszman (Medical University of Silesia)H-Index: 18
Last. Dorota Wrześniok (Medical University of Silesia)H-Index: 13
view all 6 authors...
Abstract Tetracycline is a semisynthetic antibiotic and is used in several types of infections against both gram-positive and gram-negative bacteria. This therapy is often associated with phototoxic reactions that occur after exposure to UV radiation and lead to photo-onycholysis, pseudoporphyria, solar urticaria and the fixed drug eruption in the skin. The phototoxic reactions may be related to the melanin content which, on one side may bind drugs – leading to their accumulation, and on the oth...
7 CitationsSource
#1Michał Otręba (Medical University of Silesia)H-Index: 9
#2Magdalena Zdybel (Medical University of Silesia)H-Index: 7
Last. Ewa Buszman (Medical University of Silesia)H-Index: 18
view all 7 authors...
The purpose of this study was to estimate the effect of chlorpromazine on free radical concentration in HEMn-DP melanocytes using electron paramagnetic resonance (EPR) spectroscopy. It was found that chlorpromazine at concentrations of 1 × 10−7 and 1 × 10−6 M contributed to the formation of free radicals (g values ~2) in a dose-dependent manner. The increase in free radical formation was accompanied by an increase in cytotoxicity, as shown by a tetrazolium assay. Homogeneous broadening of EPR li...
7 CitationsSource
#1Artur Beberok (Medical University of Silesia)H-Index: 9
#2Dorota Wrześniok (Medical University of Silesia)H-Index: 13
Last. Ewa Buszman (Medical University of Silesia)H-Index: 18
view all 6 authors...
Fluoroquinolone antibiotics provide broad-spectrum coverage for a number of infectious diseases, including respiratory as well as urinary tract infections. One of the important adverse effects of these drugs is phototoxicity which introduces a serious limitation to their use. To gain insight the molecular mechanisms underlying the fluoroquinolones-induced phototoxic side effects, the impact of two fluoroquinolone derivatives with different phototoxic potential, norfloxacin and moxifloxacin, on m...
16 CitationsSource
#1Michał Otręba (Medical University of Silesia)H-Index: 9
#2Dorota Wrześniok (Medical University of Silesia)H-Index: 13
Last. Ewa Buszman (Medical University of Silesia)H-Index: 18
view all 5 authors...
Abstract Chlorpromazine is used in the treatment of schizophrenia and psychotic disorders and belongs to phenothiazine class of neuroleptic drugs. It shows severe side effects such as extrapyramidal symptoms as well as ocular and skin disorders, but the mechanism is still not fully established. The aim of this study was to examine the effect of chlorpromazine on cell viability, melanogenesis and antioxidant defense system in normal human melanocytes. It has been demonstrated that chlorpromazine ...
10 CitationsSource
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