Junko Okahara
Central Institute for Experimental Animals
Embryonic stem cellMarmosetCallithrixBiologyCell biology
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Publications 10
#1Seiji Shiozawa (Keio: Keio University)H-Index: 6
#2Mayutaka Nakajima (Keio: Keio University)H-Index: 1
Last. Yohei SasagawaH-Index: 7
view all 13 authors...
Mammalian pluripotent stem cells are thought to exist in two states: naive and primed states. Generally, unlike those in rodents, pluripotent stem cells in primates including humans are regarded as being in the primed pluripotent state. Recently, several groups reported the existence of naive pluripotent stem cells in humans. In this study, we report the conversion of primed state embryonic stem cells from common marmoset, a New World monkey, to the naive state by using transgenes. The cells sho...
#1Sho Yoshimatsu (Keio: Keio University)H-Index: 3
#2Junko Okahara (Central Institute for Experimental Animals)H-Index: 6
Last. Hideyuki Okano (Keio: Keio University)H-Index: 113
view all 9 authors...
Genome editing technology greatly facilitates the genetic modification of various cells and animals. The common marmoset (Callithrix jacchus), a small non-human primate which exhibits high reproductive efficiency, is a widely used animal model in biomedical research. Developing genome editing techniques in the common marmoset will further enhance its utility. Here, we report the successful establishment of a knock-in (KI) method for marmoset embryonic stem cells (ESCs), which is based on the CRI...
4 CitationsSource
#1Mayutaka Nakajima (Keio: Keio University)H-Index: 1
#2Sho YoshimatsuH-Index: 3
Last. Hideyuki Okano (Keio: Keio University)H-Index: 113
view all 8 authors...
Abstract The common marmoset (marmoset; Callithrix jacchus) shows anatomical and physiological features that are in common with humans. Establishing induced pluripotent stem cells (iPSCs) from marmosets holds promise for enhancing the utility of the animal model for biomedical and preclinical studies. However, in spite of the presence of some previous reports on marmoset iPSCs, the reprogramming technology in marmosets is still under development. In particular, the efficacy of RNA-based reprogra...
1 CitationsSource
#1Hirohito Ishigaki (Shiga University of Medical Science)H-Index: 14
#2Toshinaga Maeda (Shiga University of Medical Science)H-Index: 9
Last. Kazumasa Ogasawara (Shiga University of Medical Science)H-Index: 31
view all 12 authors...
Immune surveillance is a critical component of the antitumor response in vivo , yet the specific components of the immune system involved in this regulatory response remain unclear. In this study, we demonstrate that autoantibodies can mitigate tumor growth in vitro and in vivo . We generated two cancer cell lines, embryonal carcinoma and glioblastoma cell lines, from monkey-induced pluripotent stem cells (iPSC) carrying a homozygous haplotype of major histocompatibility complex (MHC, Mafa in Ma...
2 CitationsSource
#1Tomoko Sakai (Japan Society for the Promotion of Science)H-Index: 6
#2Yuji Komaki (Central Institute for Experimental Animals)H-Index: 13
Last. Hideyuki Okano (Keio: Keio University)H-Index: 113
view all 11 authors...
Abstract The corpus callosum (CC) is present in all primate brains and is the major white matter tract connecting the cerebral hemispheres for integration of sensory, motor and higher-order cognitive information. The midsagittal area of the CC has frequently been used as a sensitive biomarker of brain development. Although the marmoset has been considered as an alternative non-human primate model for neuroscience research, the developmental patterns of the CC have not been explored. The present ...
5 CitationsSource
#1Jung Eun ParkH-Index: 2
#2Xian Feng ZhangH-Index: 2
Last. Afonso C. SilvaH-Index: 36
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Chronic monitoring of neuronal activity in the living brain with optical imaging techniques became feasible owing to the continued development of genetically encoded calcium indicators (GECIs). Here we report for the first time the successful generation of transgenic marmosets (Callithrix jacchus), an important nonhuman primate model in neurophysiological research, which were engineered to express the green fluorescent protein (GFP)-based family of GECIs, GCaMP, under control of either the CMV o...
31 CitationsSource
#1Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
#2Ryo Oiwa (Central Institute for Experimental Animals)H-Index: 5
Last. Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
view all 26 authors...
Recent advances in genome editing have facilitated the generation of nonhuman primate (NHP) models, with potential to unmask the complex biology of human disease not revealed by rodent models. However, their broader use is hindered by the challenges associated with generation of adult NHP models as well as the cost of their production. Here, we describe the generation of a marmoset model of severe combined immunodeficiency (SCID). This study optimized zinc-finger nucleases (ZFNs) and transcripti...
55 CitationsSource
#1Yasunari SeitaH-Index: 4
#2Tomoyuki TsukiyamaH-Index: 10
Last. Masatsugu EmaH-Index: 37
view all 19 authors...
Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body
16 CitationsSource
#1Thorsten Boroviak (Medical Research Council)H-Index: 9
#2Ruth Loos (EMBL-EBI: European Bioinformatics Institute)H-Index: 117
Last. Paul Bertone (Medical Research Council)H-Index: 48
view all 9 authors...
Naive pluripotency is manifest in the preimplantation mammalian embryo. Here we determine transcriptome dynamics of mouse development from the eight-cell stage to postimplantation using lineage-specific RNA sequencing. This method combines high sensitivity and reporter-based fate assignment to acquire the full spectrum of gene expression from discrete embryonic cell types. We define expression modules indicative of developmental state and temporal regulatory patterns marking the establishment an...
158 CitationsSource
#1Tsukasa Takahashi (Central Institute for Experimental Animals)H-Index: 1
#2Kisaburo Hanazawa (Juntendo University)H-Index: 11
Last. Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
view all 14 authors...
Intracytoplasmic sperm injection (ICSI), an important method used to treat male subfertility, is applied in the transgenic technology of sperm-mediated gene transfer. However, no study has described successful generation of offspring using ICSI in the common marmoset, a small non-human primate used as a model for biomedical translational research. In this study, we investigated blastocyst development and the subsequent live offspring stages of marmoset oocytes matured in vitro and fertilized by ...
16 CitationsSource