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Junko Okahara
Central Institute for Experimental Animals
8Publications
5H-index
210Citations
Publications 8
Newest
#1Sho Yoshimatsu (Keio: Keio University)H-Index: 1
#2Junko Okahara (Central Institute for Experimental Animals)H-Index: 5
Last.Hideyuki Okano (Keio: Keio University)H-Index: 110
view all 9 authors...
Genome editing technology greatly facilitates the genetic modification of various cells and animals. The common marmoset (Callithrix jacchus), a small non-human primate which exhibits high reproductive efficiency, is a widely used animal model in biomedical research. Developing genome editing techniques in the common marmoset will further enhance its utility. Here, we report the successful establishment of a knock-in (KI) method for marmoset embryonic stem cells (ESCs), which is based on the CRI...
#1Mayutaka Nakajima (Keio: Keio University)
#2Sho Yoshimatsu (Keio: Keio University)H-Index: 1
Last.Hideyuki Okano (Keio: Keio University)H-Index: 110
view all 8 authors...
Abstract The common marmoset (marmoset; Callithrix jacchus ) shows anatomical and physiological features that are in common with humans. Establishing induced pluripotent stem cells (iPSCs) from marmosets holds promise for enhancing the utility of the animal model for biomedical and preclinical studies. However, in spite of the presence of some previous reports on marmoset iPSCs, the reprogramming technology in marmosets is still under development. In particular, the efficacy of RNA-based reprogr...
#1Hirohito Ishigaki (Shiga University of Medical Science)H-Index: 13
#2Toshinaga Maeda (Shiga University of Medical Science)H-Index: 9
Last.Misako Nakayama (Shiga University of Medical Science)H-Index: 11
view all 12 authors...
Immune surveillance is a critical component of the antitumor response in vivo , yet the specific components of the immune system involved in this regulatory response remain unclear. In this study, we demonstrate that autoantibodies can mitigate tumor growth in vitro and in vivo . We generated two cancer cell lines, embryonal carcinoma and glioblastoma cell lines, from monkey-induced pluripotent stem cells (iPSC) carrying a homozygous haplotype of major histocompatibility complex (MHC, Mafa in Ma...
#1Tomoko Sakai (Japan Society for the Promotion of Science)H-Index: 4
#2Yuji Komaki (Central Institute for Experimental Animals)H-Index: 10
Last.Erika Sasaki (Central Institute for Experimental Animals)H-Index: 20
view all 11 authors...
Abstract The corpus callosum (CC) is present in all primate brains and is the major white matter tract connecting the cerebral hemispheres for integration of sensory, motor and higher-order cognitive information. The midsagittal area of the CC has frequently been used as a sensitive biomarker of brain development. Although the marmoset has been considered as an alternative non-human primate model for neuroscience research, the developmental patterns of the CC have not been explored. The present ...
#1Jung Eun ParkH-Index: 1
#2Xian Feng ZhangH-Index: 2
Last.Afonso C. SilvaH-Index: 35
view all 6 authors...
Chronic monitoring of neuronal activity in the living brain with optical imaging techniques became feasible owing to the continued development of genetically encoded calcium indicators (GECIs). Here we report for the first time the successful generation of transgenic marmosets (Callithrix jacchus), an important nonhuman primate model in neurophysiological research, which were engineered to express the green fluorescent protein (GFP)-based family of GECIs, GCaMP, under control of either the CMV o...
#1Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
#2Ryo Oiwa (Central Institute for Experimental Animals)H-Index: 5
Last.Kengo Sato (Keio: Keio University)H-Index: 16
view all 26 authors...
Recent advances in genome editing have facilitated the generation of nonhuman primate (NHP) models, with potential to unmask the complex biology of human disease not revealed by rodent models. However, their broader use is hindered by the challenges associated with generation of adult NHP models as well as the cost of their production. Here, we describe the generation of a marmoset model of severe combined immunodeficiency (SCID). This study optimized zinc-finger nucleases (ZFNs) and transcripti...
#1Yasunari SeitaH-Index: 3
#2Tomoyuki TsukiyamaH-Index: 8
Last.Seiji HitoshiH-Index: 17
view all 19 authors...
Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body
#1Thorsten Boroviak (Medical Research Council)H-Index: 8
#2Ruth J. F. Loos (EMBL-EBI: European Bioinformatics Institute)H-Index: 108
Last.Paul Bertone (Medical Research Council)H-Index: 46
view all 9 authors...
Naive pluripotency is manifest in the preimplantation mammalian embryo. Here we determine transcriptome dynamics of mouse development from the eight-cell stage to postimplantation using lineage-specific RNA sequencing. This method combines high sensitivity and reporter-based fate assignment to acquire the full spectrum of gene expression from discrete embryonic cell types. We define expression modules indicative of developmental state and temporal regulatory patterns marking the establishment an...
#1Tsukasa Takahashi (Central Institute for Experimental Animals)H-Index: 1
#2Kisaburo Hanazawa (Juntendo University)H-Index: 10
Last.Tomoo Eto (Central Institute for Experimental Animals)H-Index: 7
view all 14 authors...
Intracytoplasmic sperm injection (ICSI), an important method used to treat male subfertility, is applied in the transgenic technology of sperm-mediated gene transfer. However, no study has described successful generation of offspring using ICSI in the common marmoset, a small non-human primate used as a model for biomedical translational research. In this study, we investigated blastocyst development and the subsequent live offspring stages of marmoset oocytes matured in vitro and fertilized by ...
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