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Gareth Lycett
Liverpool School of Tropical Medicine
32Publications
14H-index
1,353Citations
Publications 32
Newest
#1Adriana Adolfi (LSTM: Liverpool School of Tropical Medicine)H-Index: 1
#2Beth Poulton (LSTM: Liverpool School of Tropical Medicine)
Last.Gareth Lycett (LSTM: Liverpool School of Tropical Medicine)H-Index: 14
view all 6 authors...
Resistance in Anopheles gambiae to members of all four major classes (pyrethroids, carbamates, organochlorines and organophosphates) of public health insecticides limits effective control of malaria transmission in Africa. Increased expression of detoxifying enzymes has been associated with resistance, but direct functional validation in An. gambiae has been lacking. Here we perform transgenic analysis using the GAL4/UAS system to examine insecticide resistance phenotypes conferred by increased ...
#1Amy Lynd (LSTM: Liverpool School of Tropical Medicine)H-Index: 11
#2Vasileia Balabanidou (FORTH: Foundation for Research & Technology – Hellas)H-Index: 4
Last.Gareth Lycett (LSTM: Liverpool School of Tropical Medicine)H-Index: 14
view all 7 authors...
Oenocytes are an insect cell type having diverse physiological functions ranging from cuticular hydrocarbon (CHC) production to insecticide detoxification that may impact their capacity to transmit pathogens. To develop functional genetic tools to study Anopheles gambiae oenocytes, we have trapped an oenocyte enhancer to create a transgenic mosquito Gal4 driver line that mediates tissue-specific expression. After crossing with UAS-reporter lines, An. gambiae oenocytes are fluorescently tagged th...
Abstract Cuticular hydrocarbon (CHC) biosynthesis is a major pathway of insect physiology. In Drosophila melanogaster the cytochrome P450 CYP4G1 catalyses the insect-specific oxidative decarbonylation step, while in the malaria vector Anopheles gambiae , two CYP4G paralogues, CYP4G16 and CYP4G17 are present. Analysis of the subcellular localization of CYP4G17 and CYP4G16 in larval and pupal stages revealed that CYP4G16 preserves its PM localization across developmental stages analyzed; however C...
#1Adriana Adolfi (UCI: University of California, Irvine)
#2Gareth Lycett (LSTM: Liverpool School of Tropical Medicine)H-Index: 14
Anopheles is the only genus of mosquitoes that transmit human malaria and consequently the focus of large scale genome and transcriptome-wide association studies. Genetic tools to define the function of the candidate genes arising from these analyses are vital. Moreover, genome editing offers the potential to modify Anopheles population structure at local and global scale to provide complementary tools towards the ultimate goal of malaria elimination. Major breakthroughs in Anopheles genetic ana...
#1Benjamin J. Matthews (Rockefeller University)H-Index: 10
#2Olga DudchenkoH-Index: 5
Last.Noah H. Rose (Princeton University)H-Index: 7
view all 10 authors...
Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored phys...
#1Christopher S. Spencer (LSTM: Liverpool School of Tropical Medicine)H-Index: 1
#2Cristina Yunta (LSTM: Liverpool School of Tropical Medicine)H-Index: 4
Last.Mji Paine (LSTM: Liverpool School of Tropical Medicine)H-Index: 33
view all 8 authors...
Abstract The mosquito Anopheles gambiae is the principal vector for malaria in sub-Saharan Africa. The ability of A. gambiae to transmit malaria is strictly related to blood feeding and digestion, which releases nutrients for oogenesis, as well as substantial amounts of highly toxic free heme. Heme degradation by heme oxygenase (HO) is a common protective mechanism, and a gene for HO exists in the An. gambiae genome HO (AgHO), although it has yet to be functionally examined. Here, we have cloned...
#1Adriana Adolfi (LSTM: Liverpool School of Tropical Medicine)H-Index: 1
#2Emilie Pondeville (Pasteur Institute)H-Index: 7
Last.Gareth Lycett (LSTM: Liverpool School of Tropical Medicine)H-Index: 14
view all 5 authors...
Abstract The ability to manipulate the Anopheles gambiae genome and alter gene expression effectively and reproducibly is a prerequisite for functional genetic analysis and for the development of novel control strategies in this important disease vector. However, in vivo transgenic analysis in mosquitoes is limited by the lack of promoters active ubiquitously. To address this, we used the GAL4/UAS system to investigate the promoter of the An. gambiae Polyubiquitin-c (PUBc) gene and demonstrated ...
#1Patricia Pignatelli (LSTM: Liverpool School of Tropical Medicine)H-Index: 11
#2Victoria Ingham (LSTM: Liverpool School of Tropical Medicine)H-Index: 4
Last.Hilary Ranson (LSTM: Liverpool School of Tropical Medicine)H-Index: 65
view all 6 authors...
The role of ATP-binding cassette (ABC) transporters in conferring insecticide resistance has received much attention recently. Here we identify ABC transporters differentially expressed in insecticide-resistant populations of the malaria vector, Anopheles gambiae. Although we found little evidence that the orthologues of the multidrug resistance proteins described in other species are associated with resistance in An. gambiae we did identify a subset of ABC proteins consistently differentially e...
#1Benjamin J. Matthews (Rockefeller University)H-Index: 3
#2Olga Dudchenko (BCM: Baylor College of Medicine)H-Index: 5
Last.Noah H. Rose (Princeton University)H-Index: 7
view all 10 authors...
Female Aedes aegypti mosquitoes infect hundreds of millions of people each year with dangerous viral pathogens including dengue, yellow fever, Zika, and chikungunya. Progress in understanding the biology of this insect, and developing tools to fight it, has been slowed by the lack of a high-quality genome assembly. Here we combine diverse genome technologies to produce AaegL5, a dramatically improved and annotated assembly, and demonstrate how it accelerates mosquito science and control. We anch...
#1Cyrille Ndo (University of Douala)H-Index: 5
#2Edmond Kopya (University of Yaoundé I)H-Index: 5
Last.Charles S. Wondji (LSTM: Liverpool School of Tropical Medicine)H-Index: 29
view all 7 authors...
Background Anopheles funestus is a major vector of malaria in sub-Saharan Africa. However, because it is difficult to colonize, research on this mosquito species has lagged behind other vectors, particularly the understanding of its susceptibility and interactions with the Plasmodium parasite. The present study reports one of the first experimental infections of progeny from wild-caught An. funestus with the P. falciparum parasite providing a realistic avenue for the characterisation of immune r...
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