Sylvie M. Koekkoek
University of Amsterdam
Publications 33
#1Gaby S. Steba (UvA: University of Amsterdam)H-Index: 2
#2Sylvie M. Koekkoek (UvA: University of Amsterdam)H-Index: 11
Last.William A. Paxton (University of Liverpool)H-Index: 34
view all 11 authors...
Abstract Bile-salt stimulate lipase (BSSL) is a glycoprotein found in human milk and blood that can potently bind DC-SIGN. The BSSL gene is highly polymorphic with a variant number of O-linked glycosylated 11 amino acid repeats at the C-terminus of the protein, encoded in exon 11 of the gene. It has been shown that certain BSSL genotypes associate with decreased HIV-1 transmission in vitro and decreased HIV-1 disease progression. The protein forms dimers and individuals possessing one high (typi...
#1Sabrina J. MeratH-Index: 2
#2Camille BruH-Index: 1
Last.Janke Schinkel (UvA: University of Amsterdam)H-Index: 33
view all 14 authors...
Background & Aims In order to design an effective vaccine against hepatitis C virus (HCV) infection, it is necessary to understand immune protection. A number of broadly reactive neutralizing antibodies have been isolated from B cells of HCV-infected patients. However, it remains unclear whether B cells producing such antibodies contribute to HCV clearance and long-term immune protection against HCV. Methods We analysed the B cell repertoire of 13 injecting drug users from the Amsterdam Cohort S...
1 CitationsSource
#1Gaby S. Steba (UvA: University of Amsterdam)H-Index: 2
#2Sylvie M. Koekkoek (UvA: University of Amsterdam)H-Index: 11
Last.William A. Paxton (University of Liverpool)H-Index: 34
view all 13 authors...
Background & Aims: Despite high-risk behaviour, 10%-20% of HCV multiple exposed individuals remain uninfected (MEU), whilst the remainder become infected (MEI). We hypothesize that host factors play a role in HCV susceptibility. We aimed to identify polymorphisms in host genes that encode for proteins involved in viral entry: CD81, Scavenger receptor 1 (SR-1), Low-density lipoprotein receptor (LDL-R), Claudin-1 (CLDN1), Occludin (OCLN) and Niemann-Pick C1–like 1 (NPC1L1). Methods: Multiple expos...
2 CitationsSource
#1Bernadien M. Nijmeijer (UvA: University of Amsterdam)H-Index: 1
#2Ramin Sarrami-Forooshani (UvA: University of Amsterdam)H-Index: 9
Last.Teunis B. H. Geijtenbeek (UvA: University of Amsterdam)H-Index: 58
view all 12 authors...
INTRODUCTION: The significant rise in incidence of Hepatitis C virus (HCV) infection among men-who-have-sex-with-men (MSM) living with HIV-1 suggests that HCV under specific circumstances is transmitted via sexual contact. During sexual transmission HCV has to cross the epithelial barrier to either directly enter the blood stream or indirectly via mucosal immune cells. However, the mechanisms of sexual transmission of HCV remain unclear. We investigated the role of Langerhans cells (LCs) in HCV ...
1 CitationsSource
#1Lieke Brouwer (UvA: University of Amsterdam)H-Index: 2
#2Eveliina Karelehto (UvA: University of Amsterdam)H-Index: 4
Last.Dasja Pajkrt (UvA: University of Amsterdam)H-Index: 22
view all 14 authors...
Parechoviruses (PeVs) are highly prevalent viruses worldwide. Over the last decades, several studies have been published on PeV epidemiology in Europe, Asia and North America, while information on other continents is lacking. The aim of this study was to describe PeV circulation in a cohort of children in Malawi, Africa. A total of 749 stool samples obtained from Malawian children aged 6 to 60 months were tested for the presence of PeV by real-time PCR. We performed typing by phylogenetic and Ba...
#2Norman Sachs (KNAW: Royal Netherlands Academy of Arts and Sciences)H-Index: 20
Last.Katja C. WolthersH-Index: 32
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Human enteroviruses frequently cause severe diseases in children. Human enteroviruses are transmitted via the fecal-oral route and respiratory droplets, and primary replication occurs in the gastro-intestinal and respiratory tracts; however, how enteroviruses infect these sites is largely unknown. Human intestinal organoids have recently proven to be valuable tools for studying enterovirus-host interactions in the intestinal tract. In this study, we demonstrated the susceptibility of a newly dev...
7 CitationsSource
#1Eveliina Karelehto (UvA: University of Amsterdam)H-Index: 4
#2Cosimo Cristella (UvA: University of Amsterdam)H-Index: 1
Last.Katja C. Wolthers (UvA: University of Amsterdam)H-Index: 32
view all 11 authors...
Human parechoviruses (HPeVs), a poorly studied genus within the Picornaviridae family, are classified into 19 genotypes of which HPeV1 and HPeV3 are the most often detected. HPeV1 VP1 C terminus contains an arginine-glycine-aspartic acid (RGD) motif and has been shown to depend on the host cell surface αV integrins (αV ITGs) and heparan sulfate (HS) for entry. HPeV3 lacks this motif and the receptors remain unknown. HPeVs can be detected in patient nasopharyngeal and stool samples, and infection...
2 CitationsSource
Abstract Background Human rhinoviruses (RVs) are increasingly associated with severe disease of the respiratory tract. Multiple studies highlighted the clinical significance of different RV species; RV-C is linked to asthma exacerbations and increased disease severity in children, whereas RV-B seems to correlate with milder disease. Objectives Current typing strategies for differentiation of RV species are time consuming and require extensive equipment. Here we present a novel genotyping tool to...
1 CitationsSource
We aimed to identify whether genetic polymorphisms within L-SIGN or DC-SIGN correlate with hepatitis C virus (HCV) susceptibility. A men who have sex with men (MSM) and an injecting drug users (IDU) cohort of HCV cases and multiple-exposed uninfected controls were genotyped for numerous L-SIGN and DC-SIGN polymorphisms. DC-SIGN single nucleotide polymorphisms (SNPs) -139, -871, and -939 correlated with HCV acquisition in the MSM cohort only. When the same SNPs were introduced into a transcriptio...
4 CitationsSource
#1Cynthia K. Y. HoH-Index: 6
#2Jayna Raghwani (University of Oxford)H-Index: 13
Last.Richard MolenkampH-Index: 22
view all 10 authors...
In contrast to other available next-generation sequencing platforms, PacBio single-molecule, real-time (SMRT) sequencing has the advantage of generating long reads albeit with a relatively higher error rate in unprocessed data. Using this platform, we longitudinally sampled and sequenced the hepatitis C virus (HCV) envelope genome region (1,680 nucleotides [nt]) from individuals belonging to a cluster of sexually transmitted cases. All five subjects were coinfected with HIV-1 and a closely relat...
9 CitationsSource