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Sofie De Maeseneire
Ghent University
Synthetic biologySaccharomyces cerevisiaeYeastBiochemistryBiology
75Publications
12H-index
528Citations
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Publications 75
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#1Sofie Lodens (UGent: Ghent University)H-Index: 4
#2Sophie Roelants (UGent: Ghent University)H-Index: 10
Last. Wim Soetaert (UGent: Ghent University)H-Index: 38
view all 7 authors...
Starmerella bombicola very efficiently produces the secondary metabolites sophorolipids (SLs). Their biosynthesis is not-growth associated and highly upregulated in the stationary phase. Despite high industrial and academic interest, the underlying regulation of SL biosynthesis remains unknown. In this paper, potential regulation of SL biosynthesis through the telomere positioning effect (TPE) was investigated, as the SL gene cluster is located adjacent to a telomere. An additional copy of this ...
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#1Yatti De Nijs (UGent: Ghent University)
#2Sofie De Maeseneire (UGent: Ghent University)H-Index: 12
Last. Wim Soetaert (UGent: Ghent University)H-Index: 38
view all 3 authors...
When developing industrial biotechnology processes, Saccharomyces cerevisiae (baker's yeast or brewer's yeast) is a popular choice as a microbial host. Many tools have been developed in the fields of synthetic biology and metabolic engineering to introduce heterologous pathways and tune their expression in yeast. Such tools mainly focus on controlling transcription, whereas post-transcriptional regulation is often overlooked. Herein we discuss regulatory elements found in the 5' untranslated reg...
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#1Sofie Lodens (UGent: Ghent University)H-Index: 4
#2Sophie Roelants (UGent: Ghent University)H-Index: 10
Last. Wim Soetaert (UGent: Ghent University)H-Index: 38
view all 14 authors...
Glucolipids (GLs) are glycolipid biosurfactants with promising properties. These GLs are composed of glucose attached to a hydroxy fatty acid through a omega and/or omega-1 glycosidic linkage. Up until today these interesting molecules could only be produced using an engineered Starmerella bombicola strain ( increment ugtB1::URA3 G9) producing GLs instead of sophorolipids, albeit with a very low average productivity (0.01 g center dot L-1 center dot h(-1)). In this study, we investigated the rea...
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#1Thomas Decoene (UGent: Ghent University)H-Index: 2
#2Sofie De Maeseneire (UGent: Ghent University)H-Index: 12
Last. Marjan De Mey (UGent: Ghent University)H-Index: 23
view all 3 authors...
Altering gene expression regulation by promoter engineering is a very effective way to fine-tune heterologous pathways in eukaryotic hosts. Typically, pathway building approaches in yeast still use a limited set of long, native promoters. With the today’s introduction of longer and more complex pathways, an expansion of this synthetic biology toolbox is necessary. In this study we elucidated the core promoter structure of the well-characterized yeast TEF1 promoter and determined the minimal leng...
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#1Marilyn De Graeve (UGent: Ghent University)H-Index: 1
#2Isabelle Van de Velde (UGent: Ghent University)
Last. Wim Soetaert (UGent: Ghent University)H-Index: 38
view all 11 authors...
Abstract To decrease our dependency for the diminishing source of fossils resources, bio-based alternatives are being explored for the synthesis of commodity and high-value molecules. One example in this ecological initiative is the microbial production of the biosurfactant sophorolipids by the yeast Starmerella bombicola. Sophorolipids are surface-active molecules mainly used as household and laundry detergents. Because S. bombicola is able to produce high titers of sophorolipids, the yeast is ...
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#1Nico Snoeck (UGent: Ghent University)H-Index: 1
#2Maarten De Mol (UGent: Ghent University)H-Index: 1
Last. Wim Soetaert (UGent: Ghent University)H-Index: 38
view all 10 authors...
Chromosomal integration of biosynthetic pathways for the biotechnological production of high-value chemicals is a necessity to develop industrial strains with a high long-term stability and a low production variability. However, the introduction of multiple transcription units into the microbial genome remains a difficult task. Despite recent advances, current methodologies are either laborious or efficiencies highly fluctuate depending on the length and the type of the construct. Here we presen...
1 CitationsSource
#1Maarten De MolH-Index: 1
Last. Wim SoetaertH-Index: 38
view all 7 authors...
#1Anke GoormansH-Index: 1
#2Nico SnoeckH-Index: 1
Last. Wim SoetaertH-Index: 38
view all 9 authors...
As a biorefinery platform host, Escherichia coli has been used extensively for the production of metabolites with commercial interest. Moreover, promoter and terminator databases are readily available as well as a wide amount of expression vectors and numerous gene editing technologies. Together with the ever-reducing cost of synthetic DNA, the range of possibilities in metabolic engineering is expanding even more. Recent advances have secured the possibility of integrating whole synthetic pathw...
#1Maarten De Mol (UGent: Ghent University)H-Index: 1
#2Nico Snoeck (UGent: Ghent University)H-Index: 1
Last. Wim Soetaert (UGent: Ghent University)H-Index: 38
view all 4 authors...
Abstract The struggle of humans versus pathogens is a never ending battle. Since the discovery of antibiotics humans have tipped the scales in their favour, but today bacteria are nullifying this advantage by developing resistance mechanisms against these molecules. The plethora of different antibiotics active against pathogens is shrinking while the discovery of new molecules is arduous. Especially the development of drugs active against Gram− pathogens continues slowly. New strategies to disco...
1 CitationsSource
#1Marilyn De Graeve (UGent: Ghent University)H-Index: 1
#2Sofie De Maeseneire (UGent: Ghent University)H-Index: 12
Last. Wim Soetaert (UGent: Ghent University)H-Index: 38
view all 4 authors...
In this review, we focus on one of the most important microbial producers of biosurfactants, Starmerella bombicola. Emphasis is laid on the discovery, taxonomy, habitat, cellular characteristics, biochemistry and genetics of this non-pathogenic yeast. Biosurfactants are natural surface-active compounds produced by several types of microorganisms and have been considered an interesting alternative to synthetic surfactants. The sophorolipids produced by S. bombicola are promising biosurfactants, w...
1 CitationsSource
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