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Konstantin M. Boyko
Russian Academy of Sciences
53Publications
8H-index
229Citations
Publications 53
Newest
Konstantin M. Boyko8
Estimated H-index: 8
,
Alena Yu. Nikolaeva2
Estimated H-index: 2
+ -3 AuthorsV. Popov64
Estimated H-index: 64
Published on Aug 1, 2019in Archives of Biochemistry and Biophysics 3.56
M. G. Alekseeva6
Estimated H-index: 6
(RAS: Russian Academy of Sciences),
Konstantin M. Boyko8
Estimated H-index: 8
(RAS: Russian Academy of Sciences)
+ 7 AuthorsV. N. Danilenko12
Estimated H-index: 12
(RAS: Russian Academy of Sciences)
Abstract In this study, we identified a new gene ( aph(3″)-Id ) coding for a streptomycin phosphotransferase by using phylogenetic comparative analysis of the genome of the oxytetracycline-producing strain Streptomyces rimosus ATCC 10970. Cloning the aph(3″)-Id gene in E.coli and inducing its expression led to an increase in the minimum inhibitory concentration of the recombinant E.coli strain to streptomycin reaching 350 μg/ml. To evaluate the phosphotransferase activity of the recombinant prot...
Published on Jun 1, 2019in Biochimica et Biophysica Acta 3.79
Yulia S. Zeifman3
Estimated H-index: 3
(RAS: Russian Academy of Sciences),
Konstantin M. Boyko8
Estimated H-index: 8
(RAS: Russian Academy of Sciences)
+ 4 AuthorsEkaterina Yu. Bezsudnova8
Estimated H-index: 8
(RAS: Russian Academy of Sciences)
Abstract Pyridoxal-5′-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids. Transaminases of PLP fold type IV are characterized by ( R )- or ( S )-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and ( R )-amine:pyruvate transaminases. Recently, transam...
Published on Mar 20, 2019
Konstantin M. Boyko8
Estimated H-index: 8
,
Alena Yu. Nikolaeva2
Estimated H-index: 2
+ 6 AuthorsVladimir O. Popov19
Estimated H-index: 19
Published on Mar 1, 2019in Biochimie 3.36
Ekaterina Yu. Bezsudnova8
Estimated H-index: 8
(RAS: Russian Academy of Sciences),
Konstantin M. Boyko8
Estimated H-index: 8
(RAS: Russian Academy of Sciences)
+ 4 AuthorsVladimir O. Popov19
Estimated H-index: 19
(RAS: Russian Academy of Sciences)
Abstract The high catalytic efficiency of enzymes under reaction conditions is one of the main goals in biocatalysis. Despite the dramatic progress in the development of more efficient biocatalysts by protein design, the search for natural enzymes with useful properties remains a promising strategy. The pyridoxal 5'-phosphate (PLP)-dependent transaminases represent a group of industrially important enzymes due to their ability to stereoselectively transfer amino groups between diverse substrates...
Michail N. Isupov23
Estimated H-index: 23
(University of Exeter),
Konstantin M. Boyko8
Estimated H-index: 8
(RAS: Russian Academy of Sciences)
+ 11 AuthorsAndrey V. Mardanov21
Estimated H-index: 21
(RAS: Russian Academy of Sciences)
Two new thermophilic branched chain amino acid transaminases have been identified within the genomes of different hyper-thermophilic archaea, Geoglobus acetivorans and Archaeoglobus fulgidus. These enzymes belong to the class IV of transaminases as defined by their structural fold. The enzymes have been cloned and over-expressed in Escherichia coli and the recombinant enzymes have been characterized both biochemically and structurally. Both enzymes showed high thermostability with optimal temper...
Published on Jan 1, 2019in Biochimie 3.36
Konstantin M. Boyko8
Estimated H-index: 8
(RAS: Russian Academy of Sciences),
Timur N. Baymukhametov2
Estimated H-index: 2
(Kurchatov Institute)
+ 8 AuthorsVladimir O. Popov19
Estimated H-index: 19
(RAS: Russian Academy of Sciences)
Abstract Human plasma butyrylcholinesterase (BChE) is an endogenous bioscavenger that hydrolyzes numerous medicamentous and poisonous esters and scavenges potent organophosphorus nerve agents. BChE is thus a marker for the diagnosis of OP poisoning. It is also considered a therapeutic target against Alzheimer's disease. Although the X-ray structure of a partially deglycosylated monomer of human BChE was solved 15 years ago, all attempts to determine the 3D structure of the natural full-length gl...
Published on Nov 1, 2018in Extremophiles 2.05
Tatiana E. Petrova4
Estimated H-index: 4
(Keldysh Institute of Applied Mathematics),
Konstantin M. Boyko8
Estimated H-index: 8
(RAS: Russian Academy of Sciences)
+ 7 AuthorsEkaterina Yu. Bezsudnova8
Estimated H-index: 8
(RAS: Russian Academy of Sciences)
A novel type 1 geranylgeranyl pyrophosphate synthase GACE1337 has been identified within the genome of a newly identified hyperthermophilic archaeon Geoglobus acetivorans. The enzyme has been cloned and over-expressed in Escherichia coli. The recombinant enzyme has been biochemically and structurally characterized. It is able to catalyze the synthesis of geranylgeranyl pyrophosphate as a major product and of farnesyl pyrophosphate in smaller amounts, as measured by gas chromatography–mass spectr...
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