Erika Sasaki
Central Institute for Experimental Animals
Publications 144
#1Yasuhiro Uno (Kadai: Kagoshima University)H-Index: 20
#2Shotaro Uehara (Showa Pharmaceutical University)H-Index: 16
Last.Hiroshi Yamazaki (Showa Pharmaceutical University)H-Index: 68
view all 9 authors...
Abstract UDP-glucuronosyltransferases (UGTs) are essential drug-conjugation enzymes that metabolize a variety of endobiotic and xenobiotic substrates. The molecular characteristics of UGTs have been extensively investigated in humans, but remain to be investigated in common marmosets, a nonhuman primate species widely used in drug metabolism studies. In this study, 11 UGT cDNAs (UGT1A1, 1A3, 1A4, 1A6, 1A7, and 1A9; and UGT2B49, 2B50, 2B51, 2B52, and 2B53) were isolated and characterized in marmo...
#1Lisa Hirose (UTokyo: University of Tokyo)H-Index: 2
#2Takafumi Hiramoto (UTokyo: University of Tokyo)H-Index: 2
Last.Kenzaburo Tani (UTokyo: University of Tokyo)H-Index: 37
view all 21 authors...
#1Eri Tabata (Kogakuin University)H-Index: 5
#2Akinori Kashimura (Kogakuin University)H-Index: 8
Last.Fumitaka Oyama (Kogakuin University)H-Index: 34
view all 11 authors...
Chitin is a polymer of N-acetyl-D-glucosamine (GlcNAc) and a main constituent of insects’ exoskeleton. Insects are rich in protein with high energy conversion efficiency. Recently, we have reported that acidic chitinases (Chia) act as digestive enzymes in mouse, pig and chicken (omnivorous) but not in dog (carnivorous) and bovine (herbivorous), indicating that feeding behavior affects Chia expression levels, and determines chitin digestibility in the particular animals. Common marmoset (Callithr...
2 CitationsSource
#2Satsuki FukudaH-Index: 4
Last.Masayuki ShimodaH-Index: 27
view all 6 authors...
Common marmosets have attracted considerable attention as a small standard primate model in biomedical research. However, no marmoset diabetes model is available. Here, we established a marmoset diabetes model via the combination of partial pancreatectomy and intravenous streptozotocin (STZ). A partial pancreatectomy was performed in 11 common marmosets and multiple STZ doses were intravenously administered. Diabetes was diagnosed upon sustained hyperglycaemia (nonfasting blood glucose level >20...
#1Shotaro Uehara (Showa Pharmaceutical University)H-Index: 16
#2Yasuhiro Uno (Kadai: Kagoshima University)H-Index: 20
Last.Hiroshi Yamazaki (Showa Pharmaceutical University)H-Index: 68
view all 5 authors...
Abstract Common marmoset (Callithrix jacchus) is an attractive animal model primate species for potential use in drug metabolism and pharmacokinetic studies. In this study, marmoset cytochrome P450 (P450) 2S1, 4V2, 7A1, 7B1, 8B1, 24A1, 26A1, 26C1, 27A1, 39A1, and 51A1 cDNAs were isolated from marmoset tissues (brains, lungs, livers, kidneys, and jejunums). Deduced amino acid sequences (89-98% homologous) of the marmoset P450 gene suggested similarity of molecular characteristics of marmoset P450...
#1Sho Yoshimatsu (Keio: Keio University)H-Index: 2
#2Junko Okahara (Central Institute for Experimental Animals)H-Index: 5
Last.Hideyuki Okano (Keio: Keio University)H-Index: 112
view all 9 authors...
Genome editing technology greatly facilitates the genetic modification of various cells and animals. The common marmoset (Callithrix jacchus), a small non-human primate which exhibits high reproductive efficiency, is a widely used animal model in biomedical research. Developing genome editing techniques in the common marmoset will further enhance its utility. Here, we report the successful establishment of a knock-in (KI) method for marmoset embryonic stem cells (ESCs), which is based on the CRI...
4 CitationsSource
#1Wakako Kumita (Central Institute for Experimental Animals)H-Index: 2
#2Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
Last.Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
view all 13 authors...
Genetically modified nonhuman primates (NHP) are useful models for biomedical research. Gene editing technologies have enabled production of target-gene knock-out (KO) NHP models. Target-gene-KO/knock-in (KI) efficiency of CRISPR/Cas9 has not been extensively investigated in marmosets. In this study, optimum conditions for target gene modification efficacies of CRISPR/mRNA and CRISPR/nuclease in marmoset embryos were examined. CRISPR/nuclease was more effective than CRISPR/mRNA in avoiding mosai...
1 CitationsSource
#1Takahiko Noro (Central Institute for Experimental Animals)H-Index: 10
#2Kazuhiko NamekataH-Index: 26
Last.Takayuki HaradaH-Index: 33
view all 18 authors...
The common marmoset (Callithrix jacchus) is a non-human primate that provides valuable models for neuroscience and aging research due to its anatomical similarities to humans and relatively short lifespan. This study was carried out to examine whether aged marmosets develop glaucoma, as seen in humans. We found that 11% of the aged marmosets presented with glaucoma-like characteristics; this incident rate is very similar to that in humans. Magnetic resonance imaging showed a significant volume l...
#1Sho Yoshimatsu (Keio: Keio University)H-Index: 2
#2Mari Nakamura (Keio: Keio University)H-Index: 2
Last.Hideyuki Okano (Keio: Keio University)H-Index: 112
view all 8 authors...
Abstract The common marmoset (marmoset; Callithrix jacchus) harbors various desired features as a non-human primate (NHP) model for neuroscience research. Recently, efforts have been made to induce neural cells in vitro from marmoset pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), which are characterized by their capacity to differentiate into all cell types from the three germ layers. Successful generation of marmoset neural cells...
#1Sho YoshimatsuH-Index: 2
#2Takefumi SoneH-Index: 6
Last.Hideyuki OkanoH-Index: 112
view all 10 authors...
Knock-in (KI) gene targeting can be employed for a wide range of applications in stem cell research. However, vectors for KI require multiple complicated processes for construction, including multiple times of digestion/ligation steps and extensive restriction mapping, which has imposed limitations for the robust applicability of KI gene targeting. To circumvent this issue, here we introduce versatile and systematic methods for generating KI vectors by molecular cloning. In this approach, we emp...