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Konstantin O. Nagornov
École Polytechnique Fédérale de Lausanne
Analytical chemistryMass spectrometryFourier transform ion cyclotron resonanceChemistryIon cyclotron resonance
14Publications
6H-index
113Citations
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Publications 15
Newest
#2Markus Zennegg (Empa: Swiss Federal Laboratories for Materials Science and Technology)H-Index: 26
view all 5 authors...
The range of commercial techniques for high-resolution gas-chromatography–mass spectrometry (GC–MS) has been recently extended with the introduction of GC Orbitrap Fourier transform mass spectrometry (FTMS). We report on progress with quantitation performance in the analysis of persistent organic pollutants (POP), by averaging of time-domain signals (transients), from a number of GC–FTMS experiment replicates. Compared to a standard GC–FTMS measurement (a single GC–FTMS experiment replicate, mas...
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#1Daisy Unsihuay (Purdue University)
#2Jiamin Qiu (Purdue University)
Last. Julia Laskin (Purdue University)H-Index: 48
view all 8 authors...
Abstract Nonpolar triglycerides (TGs) are rarely detected in mass spectrometry imaging (MSI) experiments unless they are abundant in the sample. Herein, we use nanospray desorption electrospray ionization (nano-DESI) to explore the role of the solvent composition and ionic dopants on the detection of TGs in a murine gastrocnemius muscle tissue used as a model system. We evaluated three solvent mixtures for their ability to extract nonpolar TG species: MeOH:H2O 9:1 (v/v), MeOH:DCM 6:4 (v/v) and M...
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#1Sunitha Narasimha (UNIL: University of Lausanne)H-Index: 7
#2Konstantin O. Nagornov (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 6
Last. Roshan K. Vijendravarma (UNIL: University of Lausanne)H-Index: 10
view all 10 authors...
Oviparous animals across many taxa have evolved diverse strategies that deter egg predation, providing valuable tests of how natural selection mitigates direct fitness loss. Communal egg laying in nonsocial species minimizes egg predation. However, in cannibalistic species, this very behavior facilitates egg predation by conspecifics (cannibalism). Similarly, toxins and aposematic signaling that deter egg predators are often inefficient against resistant conspecifics. Egg cannibalism can be adap...
4 CitationsSource
#1Pieter C. Kooijman (UM: Maastricht University)H-Index: 1
#2Konstantin O. Nagornov (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 6
Last. Shane R. Ellis (UM: Maastricht University)H-Index: 14
view all 7 authors...
Desorption electrospray ionisation-mass spectrometry imaging (DESI-MSI) is a powerful imaging technique for the analysis of complex surfaces. However, the often highly complex nature of biological samples is particularly challenging for MSI approaches, as options to appropriately address mass spectral complexity are limited. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) offers superior mass accuracy and mass resolving power, but its moderate throughput inhibits broader ...
1 CitationsSource
#1Yury O. Tsybin (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 26
#2Konstantin O. Nagornov (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 6
Last. Anton N. Kozhinov (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 8
view all 3 authors...
Abstract Fourier transform mass spectrometry (FTMS) is an enabling technique to many challenging applications in molecular sciences. In this chapter we revisit FTMS fundamentals to further rationalize the selection of data acquisition and data processing approaches for the everyday FTMS practice. Whereas the FT-ICR MS fundamentals have been investigated and extensively reported for more than 40 years, the same level of understanding and description is not available for the teenager and rapidly e...
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#1Kristina Srzentić (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 8
#2Konstantin O. Nagornov (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 6
Last. Yury O. Tsybin (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 26
view all 12 authors...
Pairing light and heavy chains in monoclonal antibodies (mAbs) using top-down (TD) or middle-down (MD) mass spectrometry (MS) may complement the sequence information on single chains provided by high-throughput genomic sequencing and bottom-up proteomics, favoring the rational selection of drug candidates. The 50 kDa F(ab) subunits of mAbs are the smallest structural units that contain the required information on chain pairing. These subunits can be enzymatically produced from whole mAbs and int...
8 CitationsSource
#1Joshua A. Driver (UGA: University of Georgia)H-Index: 1
#2Konstantin O. Nagornov (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 6
Last. I. Jonathan Amster (UGA: University of Georgia)H-Index: 30
view all 6 authors...
The current paradigm in FT-ICR cell design is to approximate the ideal three-dimensional quadratic trapping potential as closely as possible to maintain ion cloud spatial coherence and achieve long transients, either with hyperbolically shaped electrodes, shimming electrodes, or by dynamic harmonization. In sharp contrast, the FT-ICR analyzer cell with four narrow aperture detection electrodes (NADEL) introduces significant anharmonic terms to the trapping potential. This analyzer cell is capabl...
1 CitationsSource
#1Konstantin O. Nagornov (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 6
#2Anton N. Kozhinov (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 8
Last. Yury O. Tsybin (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 26
view all 3 authors...
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) at the cyclotron frequency instead of the reduced cyclotron frequency has been experimentally demonstrated using narrow aperture detection electrode (NADEL) ICR cells. Here, based on the results of SIMION simulations, we provide the initial mechanistic insights into the cyclotron frequency regime generation in FT-ICR MS. The reason for cyclotron frequency regime is found to be a new type of a collective motion of ions with a...
1 CitationsSource
#1Kai Schuhmann (MPG: Max Planck Society)H-Index: 12
#2Kristina SrzentićH-Index: 8
Last. Andrej Shevchenko (MPG: Max Planck Society)H-Index: 90
view all 8 authors...
Lipidomes undergo permanent extensive remodeling, but how the turnover rate differs between lipid classes and molecular species is poorly understood. We employed metabolic 15N labeling and shotgun ultra-high-resolution mass spectrometry (sUHR) to quantify the absolute (molar) abundance and determine the turnover rate of glycerophospholipids and sphingolipids by direct analysis of total lipid extracts. sUHR performed on a commercial Orbitrap Elite instrument at the mass resolution of 1.35 × 106 (...
5 CitationsSource
#1Kai Schuhmann (MPG: Max Planck Society)H-Index: 12
#2Henrik Thomas (MPG: Max Planck Society)H-Index: 12
Last. Andrej Shevchenko (MPG: Max Planck Society)H-Index: 90
view all 6 authors...
Shotgun lipidomics relies on the direct infusion of total lipid extracts into a high resolution tandem mass spectrometer. A single shotgun analysis produces several hundred of densely populated FT MS and FT MS/MS spectra, each of which might comprise thousands of peaks although a very small percentage of those belong to lipids. Eliminating noise by adjusting a minimal peak intensity threshold is biased and inefficient since lipid species and classes vary in their natural abundance and ionization...
14 CitationsSource
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