Felix E. Kemmerich
Leipzig University
Magnetic tweezersHelicaseDNADNA replicationBiology
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Publications 5
#1Felix E. Kemmerich (Leipzig University)H-Index: 4
#2Kristina Kasaciunaite (Leipzig University)H-Index: 2
Last. Ralf Seidel (Leipzig University)H-Index: 37
view all 3 authors...
Abstract Magnetic tweezers provide a versatile toolkit supporting the mechanistic investigation of helicases. In the present article, we show that custom magnetic tweezers setups are straightforward to construct and can easily be extended to provide adaptable platforms, capable of addressing a multitude of enquiries regarding the functions of these fascinating molecular machines. We first address the fundamental components of a basic magnetic tweezers scheme and review some previous results to d...
9 CitationsSource
#1Felix E. Kemmerich (WWU: University of Münster)H-Index: 4
#2Peter Daldrop (WWU: University of Münster)H-Index: 3
Last. Ralf Seidel (WWU: University of Münster)H-Index: 37
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Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release...
12 CitationsSource
#1Felix E. KemmerichH-Index: 4
#2Marko SwobodaH-Index: 14
Last. Michael SchlierfH-Index: 17
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We present a hybrid single-molecule technique combining magnetic tweezers and Forster resonance energy transfer (FRET) measurements. Through applying external forces to a paramagnetic sphere, we induce conformational changes in DNA nanostructures, which are detected in two output channels simultaneously. First, by tracking a magnetic bead with high spatial and temporal resolution, we observe overall DNA length changes along the force axis. Second, the measured FRET efficiency between two fluores...
30 CitationsSource
#1Daniel Klaue (TUD: Dresden University of Technology)H-Index: 7
#2Daniela Kobbe (KIT: Karlsruhe Institute of Technology)H-Index: 9
Last. Ralf Seidel (WWU: University of Münster)H-Index: 37
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RecQ helicases have essential roles in maintaining genome stability during replication and in controlling double-strand break repair by homologous recombination. Little is known about how the different RecQ helicases found in higher eukaryotes achieve their specialized and partially opposing functions. Here, we investigate the DNA unwinding of RecQ helicases from Arabidopsis thaliana, AtRECQ2 and AtRECQ3 at the single-molecule level using magnetic tweezers. Although AtRECQ2 predominantly unwinds...
34 CitationsSource