Andrew S. Liss
Harvard University
Publications 50
#1James B. Kobler (Harvard University)H-Index: 29
#2Monica A. Tynan (Harvard University)
Last.Tannin A. Schmidt (University of Connecticut Health Center)H-Index: 25
view all 7 authors...
#1Matteo Ligorio (Harvard University)H-Index: 9
#2Srinjoy Sil (Harvard University)H-Index: 4
Last.David T. Ting (Harvard University)H-Index: 26
view all 48 authors...
Summary Single-cell technologies have described heterogeneity across tissues, but the spatial distribution and forces that drive single-cell phenotypes have not been well defined. Combining single-cell RNA and protein analytics in studying the role of stromal cancer-associated fibroblasts (CAFs) in modulating heterogeneity in pancreatic cancer (pancreatic ductal adenocarcinoma [PDAC]) model systems, we have identified significant single-cell population shifts toward invasive epithelial-to-mesenc...
#1Liza Konnikova (Harvard University)H-Index: 7
#2Gilles Boschetti (ISMMS: Icahn School of Medicine at Mount Sinai)H-Index: 1
Last.Scott B. Snapper (Harvard University)H-Index: 52
view all 19 authors...
Simultaneous analyses of peripheral and mucosal immune compartments can yield insight into the pathogenesis of mucosal-associated diseases. Although methods to preserve peripheral immune cells are well established, studies involving mucosal immune cells have been hampered by lack of simple storage techniques. We provide a cryopreservation protocol allowing for storage of gastrointestinal (GI) tissue with preservation of viability and functionality of both immune and epithelial cells. These metho...
#1Krushna C. Patra (Harvard University)H-Index: 9
#2Yasutaka Kato (Harvard University)H-Index: 3
Last.Nabeel Bardeesy (Harvard University)H-Index: 71
view all 18 authors...
G protein αs (GNAS) mediates receptor-stimulated cAMP signalling, which integrates diverse environmental cues with intracellular responses. GNAS is mutationally activated in multiple tumour types, although its oncogenic mechanisms remain elusive. We explored this question in pancreatic tumourigenesis where concurrent GNAS and KRAS mutations characterize pancreatic ductal adenocarcinomas (PDAs) arising from intraductal papillary mucinous neoplasms (IPMNs). By developing genetically engineered mou...
Pancreatic ductal adenocarcinoma has a heterogeneous genetic landscape, marked by frequent mutation of KRAS, CDKN2A, TP53, and SMAD4, resulting in poor responses to conventional therapeutic regimens. Over the past decade, increased understanding of the genetic underpinnings of this lethal cancer has yielded several different characterizations of pancreatic cancer subtypes. However, not all phenotypes and changes in pancreatic cancer can be explained by these findings. New insights on epigenetic ...
#1Laura V. Danai (MIT: Massachusetts Institute of Technology)H-Index: 13
#2Ana Babic (Harvard University)H-Index: 11
Last.Mathew G.Vander Heiden (MIT: Massachusetts Institute of Technology)H-Index: 67
view all 29 authors...
Malignancy is accompanied by changes in the metabolism of both cells and the organism1,2. Pancreatic ductal adenocarcinoma (PDAC) is associated with wasting of peripheral tissues, a metabolic syndrome that lowers quality of life and has been proposed to decrease survival of patients with cancer3,4. Tissue wasting is a multifactorial disease and targeting specific circulating factors to reverse this syndrome has been mostly ineffective in the clinic5,6. Here we show that loss of both adipose and ...