Andrew D. Beavis
University of Toledo Medical Center
22Publications
13H-index
1,078Citations
Publications 22
Newest
Published on Feb 6, 2004in Journal of Biological Chemistry 4.01
Andrew D. Beavis13
Estimated H-index: 13
(University of Toledo Medical Center),
M F Powers4
Estimated H-index: 4
(University of Toledo Medical Center)
Abstract The mitochondrial inner membrane anion channel (IMAC) carries a wide variety of anions and is postulated to be involved in mitochondrial volume homeostasis in conjunction with the K+/H+ antiporter, thus allowing the respiratory chain proton pumps to drive salt efflux. How it is regulated is uncertain; however, it is inhibited by matrix Mg2+ and matrix protons. Previously determined values for the IC50 suggested that the channel would be closed under physiological conditions. In a previo...
27 Citations Source Cite
Published on May 23, 1997in Journal of Biological Chemistry 4.01
Yun Lu2
Estimated H-index: 2
(University of Toledo Medical Center),
Andrew D. Beavis13
Estimated H-index: 13
(University of Toledo Medical Center)
Abstract Peptides with sequences based on the leader sequence of yeast cytochrome c oxidase subunit IV (pCOX IV-(1–25)) activate the electrophoretic uptake of K+ and other cations such as tetraethylammonium and lysine by rat liver mitochondria with EC50 = 11–15 μm. Uptake of these cations is dependent on respiration and is prevented by uncoupling agents, and the V max for K+ is 1.2–1.5 μmol/min/mg. Albeit more slowly, the non-electrolytes mannitol and sucrose are also transported by this pathway...
13 Citations Source Cite
Published on Apr 1, 1996in Journal of Bioenergetics and Biomembranes 2.91
Andrew D. Beavis13
Estimated H-index: 13
(University of Toledo Medical Center),
Hamid Davatol-Hag1
Estimated H-index: 1
(University of Toledo Medical Center)
The mitochondrial inner membrane anion channel (IMAC) is a channel, identified by flux studies in intact mitochondria, which has a broad anion selectivity and is maintained closed or inactive by matrix Mg2+ and H+. We now present evidence that this channel, like many other chloride/anion channels, is reversibly blocked/inhibited by stilbene-2,2′-disulfonates. Inhibition of malonate transport approaches 100% with IC50 values of 26, 44, and 88 ΜM for DIDS, H2-DIDS, and SITS respectively and Hill c...
52 Citations Source Cite
Published on Oct 11, 1996in Journal of Biological Chemistry 4.01
Guoying Liu1
Estimated H-index: 1
(University of Toledo Medical Center),
Bryan Hinch1
Estimated H-index: 1
(University of Toledo Medical Center),
Andrew D. Beavis13
Estimated H-index: 13
(University of Toledo Medical Center)
Abstract α,ω-Dicarboxylates have antibacterial properties, have been used in the treatment of hyperpigmentary disorders, are active against various melanoma cell lines, and can also undergo β-oxidation. Little, however, is known about their transport. In this paper, we examine the mitochondrial transport of α,ω-dicarboxylates ranging from oxalate (DC2) to sebacate (DC10). DC2-DC10 are transported by the inner membrane anion channel (IMAC). DC6-DC10 are also transported by an electroneutral mecha...
32 Citations Source Cite
Published on Apr 8, 1994in Journal of Biological Chemistry 4.01
M F Powers4
Estimated H-index: 4
,
L L Smith1
Estimated H-index: 1
,
Andrew D. Beavis13
Estimated H-index: 13
Abstract The mitochondrial inner membrane anion channel (IMAC) is a transport pathway which is believed to be involved in mitochondrial volume homeostasis. The protein, however, has not been identified. In this paper, we examine the relationship between IMAC and the adenine nucleotide translocator. Many inhibitors of the adenine nucleotide translocase are shown to block IMAC, including Cibacron blue 3GA, bromcresol green, alizarin red S, agaric acid, palmitoyl-CoA, and the fluorescein derivative...
13 Citations
Published on Jul 1, 1994in Biochimica et Biophysica Acta 3.44
Ajith A. Welihinda2
Estimated H-index: 2
(University of Toledo Medical Center),
Andrew D. Beavis13
Estimated H-index: 13
(University of Toledo Medical Center),
Robert J. Trumbly12
Estimated H-index: 12
(University of Toledo Medical Center)
Abstract A Saccharomyces cerevisiae mutant, lisI-1 , hypersensitive to Li + and Na + was isolated from a wild-type strain after ethylmethane sulfonate mutagenesis. The rates of Li + and Na + uptake of the mutant are about 3–4-times higher than that of the wild-type; while the rates of cation efflux from the mutant and wild-type strains are indistinguishable. The LIS1 was isolated from a yeast genomic library by complementation of the cation hypersensitivity of the lisI-1 strain. LIS1 is a single...
52 Citations Source Cite
Published on Jan 15, 1993in Journal of Biological Chemistry 4.01
Andrew D. Beavis13
Estimated H-index: 13
,
Yun Lu2
Estimated H-index: 2
,
K. D. Garlid1
Estimated H-index: 1
Respiring mitochondria drive the electrophoretic uptake of K+ and other cations. In the presence of permeant acids this transport leads to mitochondrial swelling if it is not compensated by electroneutral K+/ H+ exchange mediated by the K+/H+ antiporter. The mechanism of influx has yet to be established; however, evidence is accumulating that in addition to leak pathways a specific K’ channel or uniporter may be involved. We examine some of the properties of K+ uniport which are consistent with ...
81 Citations
Published on Oct 1, 1993in Biochimica et Biophysica Acta 3.44
Ajith A. Welihinda2
Estimated H-index: 2
(University of Toledo Medical Center),
Robert J. Trumbly12
Estimated H-index: 12
(University of Toledo Medical Center)
+ 1 AuthorsAndrew D. Beavis13
Estimated H-index: 13
(University of Toledo Medical Center)
Abstract Unlike mammalian mitochondria, yeast mitochondria swell spontaneously in both NaOAc and KOAc. This swelling reflects the activity of an electroneutral cation/H + antiport pathway. Transport of neither salt is stimulated by depletion of endogenous divalent cations; however, it can be inhibited by addition of exogenous divalent cations (Mg 2+ IC 50 = 2.08 mM, Ca 2+ IC 50 = 0.82 mM). Transport of both Na + and K + can be completely inhibited by the amphiphilic amines propranolol (IC 50 = 7...
22 Citations Source Cite
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