Bernard Lorber
University of Strasbourg
Publications 123
#1Igor OrlovH-Index: 7
#2Caroline HemmerH-Index: 4
Last.Lorène Belval (INRA: Institut national de la recherche agronomique)
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Grapevine fanleaf virus (GFLV) is a picorna-like plant virus transmitted by nematodes that affects vineyards worldwide. Nanobody (Nb)-mediated resistance against GFLV has been created recently and shown to be highly effective in plants including grapevine, but the underlying mechanism is unknown. Here we present the high-resolution cryo-EM structure of the GFLV-Nb23 complex which provides the basis for the molecular recognition by the nanobody. The structure reveals a composite binding site brid...
#1Christophe André (University of Bordeaux)
#2Isabelle Martiel (Paul Scherrer Institute)H-Index: 8
Last.Vincent Olieric (Paul Scherrer Institute)H-Index: 14
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Bacterial sliding clamps control the access of DNA polymerases to the replication fork and are appealing targets for antibacterial drug development. It is therefore essential to decipher the polymerase-clamp binding mode across various bacterial species. Here, two residues of the E. coli clamp binding pocket, EcS346 and EcM362, and their cognate residues in M. tuberculosis and B. subtilis clamps, were mutated. The effects of these mutations on the interaction of a model peptide with these varian...
#1R. de Wijn (UDS: University of Strasbourg)
#2Raphaël de WijnH-Index: 1
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Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine `non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard te...
#1Caroline Hemmer (UDS: University of Strasbourg)H-Index: 4
#2Samia Djennane (UDS: University of Strasbourg)H-Index: 3
Last.Mireille Perrin (UDS: University of Strasbourg)H-Index: 2
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Summary Since their discovery, single-domain antigen-binding fragments of camelid-derived heavy-chain-only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited. Many plant viruses including Grapevine fanleaf virus (GFLV), a nematode-transmitted icosahedral virus and causal agent of fanleaf degenerative disease, have worldwide distribution a...