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Bernard Lorber
University of Strasbourg
ChemistryCrystallizationBiochemistryCrystalCrystallography
107Publications
27H-index
2,057Citations
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Publications 122
Newest
#1Igor OrlovH-Index: 7
#2Caroline Hemmer (UDS: University of Strasbourg)H-Index: 5
Last. Lorène BelvalH-Index: 5
view all 22 authors...
Grapevine fanleaf virus (GFLV) is a picorna-like plant virus transmitted by nematodes that affects vineyards worldwide. Nanobody (Nb)-mediated resistance against GFLV has been created recently, and shown to be highly effective in plants, including grapevine, but the underlying mechanism is unknown. Here we present the high-resolution cryo electron microscopy structure of the GFLV–Nb23 complex, which provides the basis for molecular recognition by the Nb. The structure reveals a composite binding...
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#1Raphaël de WijnH-Index: 1
Last. Eric GirardH-Index: 21
view all 13 authors...
The reproducible preparation of well-diffracting crystals is a prerequisite for every structural study based on crystallography. An instrument called XtalController has recently been designed that allows the monitoring of crystallization assays using dynamic light scattering and microscopy, and integrates piezo pumps to alter the composition of the mother liquor during the experiment. We have applied this technology to study the crystallization of two enzymes, the CCA-adding enzyme of the psychr...
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#1Igor OrlovH-Index: 7
#2Caroline Hemmer (UDS: University of Strasbourg)H-Index: 5
Last. Bruno P. KlaholzH-Index: 22
view all 22 authors...
Abstract Grapevine fanleaf virus (GFLV) is a picorna-like plant virus transmitted by nematodes that affects vineyards worldwide. Nanobody (Nb)-mediated resistance against GFLV has been created recently and shown to be highly effective in plants including grapevine, but the underlying mechanism is unknown. Here we present the high-resolution cryo-EM structure of the GFLV-Nb23 complex which provides the basis for the molecular recognition by the nanobody. The structure reveals a composite binding ...
Source
#1Christophe André (University of Bordeaux)
#2Isabelle Martiel (Paul Scherrer Institute)H-Index: 10
Last. Dominique Burnouf (UDS: University of Strasbourg)H-Index: 16
view all 12 authors...
Bacterial sliding clamps control the access of DNA polymerases to the replication fork and are appealing targets for antibacterial drug development. It is therefore essential to decipher the polymerase-clamp binding mode across various bacterial species. Here, two residues of the E. coli clamp binding pocket, EcS346 and EcM362, and their cognate residues in M. tuberculosis and B. subtilis clamps, were mutated. The effects of these mutations on the interaction of a model peptide with these varian...
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#1R. de Wijn (UDS: University of Strasbourg)H-Index: 1
#2O. Hennig (Leipzig University)H-Index: 2
Last. Claude Sauter (UDS: University of Strasbourg)H-Index: 24
view all 17 authors...
Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine `non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard te...
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#1Raphaël de Wijn (UDS: University of Strasbourg)H-Index: 1
#2Oliver Hennig (Leipzig University)H-Index: 1
Last. Claude Sauter (UDS: University of Strasbourg)H-Index: 24
view all 7 authors...
The determination of conditions for the reproducible growth of well diffracting crystals is a critical step in every biocrystallographic study. On the occasion of a new structural biology project, several advanced crystallogenesis approaches were tested in order to increase the success rate of crystallization. These methods included screening by microseed matrix screening, optimization by counter-diffusion and crystal detection by trace fluorescent labeling, and are easily accessible to any labo...
2 CitationsSource
#1Caroline Hemmer (UDS: University of Strasbourg)H-Index: 5
#2Samia Djennane (UDS: University of Strasbourg)H-Index: 3
Last. Christophe Ritzenthaler (UDS: University of Strasbourg)H-Index: 31
view all 21 authors...
Summary Since their discovery, single-domain antigen-binding fragments of camelid-derived heavy-chain-only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited. Many plant viruses including Grapevine fanleaf virus (GFLV), a nematode-transmitted icosahedral virus and causal agent of fanleaf degenerative disease, have worldwide distribution a...
10 CitationsSource
#1Claude SauterH-Index: 24
#2Bernard LorberH-Index: 27
Last. Marie SisslerH-Index: 19
view all 9 authors...
Mutations in human mitochondrial aminoacyl-tRNA synthetases are associated with a variety of neurodegenerative disorders. The effects of these mutations on the structure and function of the enzymes remain to be established. Here, we investigate six mutants of the aspartyl-tRNA synthetase correlated with leukoencephalopathies. Our integrated strategy, combining an ensemble of biochemical and biophysical approaches, reveals that mutants are diversely affected with respect to their solubility in ce...
12 CitationsSource
#1Philippe WolffH-Index: 10
#2Ismail AmalH-Index: 3
Last. Dominique BurnoufH-Index: 16
view all 11 authors...
Bacterial sliding clamps are molecular hubs that interact with many proteins involved in DNA metabolism through their binding, via a conserved peptidic sequence, into a universally conserved pocket. This interacting pocket is acknowledged as a potential molecular target for the development of new antibiotics. We previously designed short peptides with an improved affinity for the Escherichia coli binding pocket. Here we show that these peptides differentially interact with other bacterial clamps...
21 CitationsSource
#1Franziska PinkerH-Index: 6
#2M. BrunH-Index: 12
Last. Claude SauterH-Index: 24
view all 11 authors...
17 CitationsSource
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