William A. Paxton
University of Liverpool
Publications 101
#1Gaby S. Steba (UvA: University of Amsterdam)H-Index: 2
#2Sylvie M. Koekkoek (UvA: University of Amsterdam)H-Index: 11
Last.William A. Paxton (University of Liverpool)H-Index: 34
view all 11 authors...
Abstract Bile-salt stimulate lipase (BSSL) is a glycoprotein found in human milk and blood that can potently bind DC-SIGN. The BSSL gene is highly polymorphic with a variant number of O-linked glycosylated 11 amino acid repeats at the C-terminus of the protein, encoded in exon 11 of the gene. It has been shown that certain BSSL genotypes associate with decreased HIV-1 transmission in vitro and decreased HIV-1 disease progression. The protein forms dimers and individuals possessing one high (typi...
#1Emily E. I. M. Mouser (UvA: University of Amsterdam)H-Index: 2
#2Georgios Pollakis (University of Liverpool)H-Index: 28
Last.William A. Paxton (University of Liverpool)H-Index: 34
view all 7 authors...
Parasitic helminths evade, skew and dampen human immune responses through numerous mechanisms. Such effects will likely have consequences for HIV-1 transmission and disease progression. Here we analyzed the effects that soluble egg antigen (SEA) from Schistosoma mansoni had on modulating HIV-1 infection and cytokine/chemokine production in vitro. We determined that SEA, specifically through kappa-5, can potently bind to DC-SIGN and thereby blocks DC-SIGN mediated HIV-1 trans-infection (p<0.05) w...
#1Jenny Clarke (University of Liverpool)H-Index: 1
#2Murielle Baltazar (University of Liverpool)
Last.Aras Kadioglu (University of Liverpool)H-Index: 35
view all 10 authors...
Streptococcus pyogenes (GAS) is among the most diverse of all human pathogens, responsible for a range of clinical manifestations, from mild superficial infections such as pharyngitis to serious invasive infections such as necrotising fasciitis and sepsis. The drivers of these different disease phenotypes are not known. The GAS cholesterol-dependent cytolysin, streptolysin O (SLO), has well established cell and tissue destructive activity. We investigated the role of SLO in determining disease o...
#1Gaby S. Steba (UvA: University of Amsterdam)H-Index: 2
#2Sylvie M. Koekkoek (UvA: University of Amsterdam)H-Index: 11
Last.William A. Paxton (University of Liverpool)H-Index: 34
view all 13 authors...
Background & Aims: Despite high-risk behaviour, 10%-20% of HCV multiple exposed individuals remain uninfected (MEU), whilst the remainder become infected (MEI). We hypothesize that host factors play a role in HCV susceptibility. We aimed to identify polymorphisms in host genes that encode for proteins involved in viral entry: CD81, Scavenger receptor 1 (SR-1), Low-density lipoprotein receptor (LDL-R), Claudin-1 (CLDN1), Occludin (OCLN) and Niemann-Pick C1–like 1 (NPC1L1). Methods: Multiple expos...
2 CitationsSource
#1Jordan Thomas (University of Liverpool)
#2Alessandra Ruggiero (University of Liverpool)H-Index: 3
Last.Georgios Pollakis (University of Liverpool)H-Index: 28
view all 6 authors...
HIV-1 infection cannot be cured due to the presence of the latent reservoir (LR). Novel cure or treatment strategies, such as “shock and kill” or therapeutic vaccination, aim to reduce or eradicate the LR. Cure strategies utilise robust DNA quantification assays to measure the change in the LR in low copy scenarios. No standard assay exists, which impedes the reliable comparison of results from different therapy and vaccine trials and HIV-1 total DNA quantification methods have not been previous...
#2Eva MalatinkovaH-Index: 9
Last.William A. PaxtonH-Index: 34
view all 9 authors...
#1Richard S. TedderH-Index: 31
#2Dhan Samuel (PHE: Public Health England)H-Index: 4
Last.Malcolm G. SempleH-Index: 17
view all 24 authors...
Background: Passive therapy with convalescent plasma provides an early opportunity to intervene in Ebola virus disease (EVD). Methods for field screening and selection of potential donors and quantifying plasma antibody are needed. Study Design and Methods: Recombinant Ebola virus glycoprotein (EBOV GP) was formatted into immunoglobulin G-capture, competitive, and double-antigen bridging enzyme immunoassays (EIAs). EVD survivors in Freetown, Sierra Leone, were recruited as potential plasma donor...
3 CitationsSource
We aimed to identify whether genetic polymorphisms within L-SIGN or DC-SIGN correlate with hepatitis C virus (HCV) susceptibility. A men who have sex with men (MSM) and an injecting drug users (IDU) cohort of HCV cases and multiple-exposed uninfected controls were genotyped for numerous L-SIGN and DC-SIGN polymorphisms. DC-SIGN single nucleotide polymorphisms (SNPs) -139, -871, and -939 correlated with HCV acquisition in the MSM cohort only. When the same SNPs were introduced into a transcriptio...
4 CitationsSource
#1Riddhima Banga (UNIL: University of Lausanne)H-Index: 7
#2Francesco A. Procopio (UNIL: University of Lausanne)H-Index: 13
Last.Matthieu Perreau (UNIL: University of Lausanne)H-Index: 23
view all 10 authors...
We recently demonstrated that lymph node (LN) PD-1+/T follicular helper (Tfh) cells from anti-retroviral therapy (ART) treated HIV-infected individuals were enriched in cells containing replication competent virus. However, the distribution of cells containing inducible replication competent virus has been only partially elucidated in blood memory CD4 T-cell populations including the Tfh cell counterpart circulating in blood (cTfh). In this context, we have investigated the distribution of 1) to...
10 CitationsSource
#1Alessandra Ruggiero (University of Liverpool)H-Index: 3
#2Eva Malatinkova (UGent: Ghent University)H-Index: 9
Last.Ward De Spiegelaere (UGent: Ghent University)H-Index: 15
view all 6 authors...
Numerous HIV-1 curative strategies have been proposed to eradicate the virus reservoir pool that remains integrated within target cells, despite successful antiretroviral therapy. To test the impact of such interventions on this reservoir, a universal marker of persistence is needed. Quantifying integrated HIV-1 DNA load has been proposed as a strong virological marker. In this paper, we provide a detailed description of the most commonly used assays to quantify integrated HIV-1 DNA and applicat...
2 CitationsSource