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Ningyi Shao
Stanford University
Induced pluripotent stem cellNucleus accumbensGeneticsBiologyCell biology
40Publications
19H-index
1,476Citations
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Publications 42
Newest
#1Alexa Wnorowski (Stanford University)H-Index: 3
#2Arun SharmaH-Index: 26
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 13 authors...
Summary With extended stays aboard the International Space Station (ISS) becoming commonplace, there is a need to better understand the effects of microgravity on cardiac function. We utilized human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to study the effects of microgravity on cell-level cardiac function and gene expression. The hiPSC-CMs were cultured aboard the ISS for 5.5 weeks and their gene expression, structure, and functions were compared with ground control hiPS...
2 CitationsSource
#1Joe Z. Zhang (Stanford University)H-Index: 4
#2Vittavat Termglinchan (Stanford University)H-Index: 9
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 17 authors...
Summary The diversity of cardiac lineages contributes to the heterogeneity of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CMs). Here, we report the generation of a hiPSC TBX5Clover2 and NKX2-5TagRFP double reporter to delineate cardiac lineages and isolate lineage-specific subpopulations. Molecular analyses reveal that four different subpopulations can be isolated based on the differential expression of TBX5 and NKX2-5, TBX5+NKX2-5+, TBX5+NKX2-5−, TBX5−NKX2-5+, and TBX5−N...
4 CitationsSource
#1Sang-Bing Ong (NUS: National University of Singapore)H-Index: 17
#2Won Hee Lee (UA: University of Arizona)H-Index: 1
Last. Sang-Ging Ong (UIC: University of Illinois at Chicago)H-Index: 2
view all 15 authors...
Summary The relationship between diabetes and endothelial dysfunction remains unclear, particularly the association with pathological activation of calpain, an intracellular cysteine protease. Here, we used human induced pluripotent stem cells-derived endothelial cells (iPSC-ECs) to investigate the effects of diabetes on vascular health. Our results indicate that iPSC-ECs exposed to hyperglycemia had impaired autophagy, increased mitochondria fragmentation, and was associated with increased calp...
1 CitationsSource
#1Alexa Wnorowski (Stanford University)H-Index: 3
#2Arun Sharma (Stanford University)H-Index: 26
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 12 authors...
Source
#1Joe Z. Zhang (Stanford University)H-Index: 4
#2Vittavat Termglinchan (Stanford University)H-Index: 9
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 14 authors...
Source
#1Nigel G. KooremanH-Index: 12
#2Youngkyun KimH-Index: 11
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 19 authors...
Summary Cancer cells and embryonic tissues share a number of cellular and molecular properties, suggesting that induced pluripotent stem cells (iPSCs) may be harnessed to elicit anti-tumor responses in cancer vaccines. RNA sequencing revealed that human and murine iPSCs express tumor-associated antigens, and we show here a proof of principle for using irradiated iPSCs in autologous anti-tumor vaccines. In a prophylactic setting, iPSC vaccines prevent tumor growth in syngeneic murine breast cance...
25 CitationsSource
#1Jae Cheol Lee (Stanford University)H-Index: 23
#2Ningyi Shao (Stanford University)H-Index: 19
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 15 authors...
Summary Cardiac development requires coordinated and large-scale rearrangements of the epigenome. The roles and precise mechanisms through which specific epigenetic modifying enzymes control cardiac lineage specification, however, remain unclear. Here we show that the H3K4 methyltransferase SETD7 controls cardiac differentiation by reading H3K36 marks independently of its enzymatic activity. Through chromatin immunoprecipitation sequencing (ChIP-seq), we found that SETD7 targets distinct sets of...
4 CitationsSource
#1Ming-Tao Zhao (Stanford University)H-Index: 12
#2Haodong Chen (Stanford University)H-Index: 12
Last. Joseph C. Wu (Stanford University)H-Index: 91
view all 18 authors...
Abstract Patient-specific pluripotent stem cells (PSCs) can be generated via nuclear reprogramming by transcription factors (i.e., induced pluripotent stem cells, iPSCs) or by somatic cell nuclear transfer (SCNT). However, abnormalities and preclinical application of differentiated cells generated by different reprogramming mechanisms have yet to be evaluated. Here we investigated the molecular and functional features, and drug response of cardiomyocytes (PSC-CMs) and endothelial cells (PSC-ECs)...
18 CitationsSource
#1Mingxia GuH-Index: 15
#2Lei TianH-Index: 4
Last. Marlene RabinovitchH-Index: 75
view all 8 authors...
Pulmonary Arterial Hypertension (PAH) is a progressive disorder characterized by endothelial cell (EC) apoptosis, loss of distal vessels, and occlusive vascular remodeling. Current PAH therapies im...
#1Dan LiH-Index: 8
#2Zhixin ZhaoH-Index: 3
Last. Marlene RabinovitchH-Index: 75
view all 6 authors...
Pulmonary arterial hypertension (PAH) is a life-threatening disease that is not effectively cured by current therapies. It is characterized by progressive occlusion of the vascular lumen owing to t...
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