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Nicolas Mermod
University of Lausanne
121Publications
30H-index
2,899Citations
Publications 121
Newest
Published in DNA Repair3.71
Sandra Bosshard2
Estimated H-index: 2
(UNIL: University of Lausanne),
Pierre-Olivier Duroy (UNIL: University of Lausanne), Nicolas Mermod30
Estimated H-index: 30
(UNIL: University of Lausanne)
Abstract CRISPR technologies greatly foster genome editing in mammalian cells through site-directed DNA double strand breaks (DSBs). However, precise editing outcomes, as mediated by homologous recombination (HR) repair, are typically infrequent and outnumbered by undesired genome alterations. By using knockdown and overexpression studies in Chinese hamster ovary (CHO) cells as well as characterizing repaired DNA junctions, we found that efficient HR-mediated genome editing depends on alternativ...
Published on Feb 1, 2018in Molecular Therapy8.40
Pavithra Iyer2
Estimated H-index: 2
(UNIL: University of Lausanne),
Lionel O. Mavoungou1
Estimated H-index: 1
(UNIL: University of Lausanne)
+ 9 AuthorsMałgorzata Lekka26
Estimated H-index: 26
(PAN: Polish Academy of Sciences)
Duchenne muscular dystrophy (DMD) is a lethal muscle-wasting disease currently without cure. We investigated the use of the PiggyBac transposon for full-length dystrophin expression in murine mesoangioblast (MABs) progenitor cells. DMD murine MABs were transfected with transposable expression vectors for full-length dystrophin and transplanted intramuscularly or intra-arterially into mdx/ SCID mice. Intra-arterial delivery indicated that the MABs could migrate to regenerating muscles to mediate ...
Published on Aug 1, 2017in Biotechnology and Bioengineering4.26
Xuan Droz1
Estimated H-index: 1
(UNIL: University of Lausanne),
Niamh Harraghy6
Estimated H-index: 6
(UNIL: University of Lausanne)
+ 7 AuthorsNicolas Mermod30
Estimated H-index: 30
(UNIL: University of Lausanne)
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from th...
Published on Feb 1, 2017in Biotechnology and Bioengineering4.26
Kaja Kostyrko5
Estimated H-index: 5
(UNIL: University of Lausanne),
Samuel Neuenschwander11
Estimated H-index: 11
(Swiss Institute of Bioinformatics)
+ 9 AuthorsPierre-Alain Girod14
Estimated H-index: 14
Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous recombination (HR) DNA repair pathways. However, a direct involvement of these pathways has remained unclear. Here, we show that the silencing of many HR factors enhanced plasmid concatemer formation and stable expression of the gene of interest...
Published on Apr 7, 2016in Nucleic Acids Research11.15
Kaja Kostyrko5
Estimated H-index: 5
(UNIL: University of Lausanne),
Nicolas Mermod30
Estimated H-index: 30
(UNIL: University of Lausanne)
DNA double stranded breaks (DSBs) are one of the most deleterious types of DNA lesions. The main pathways responsible for repairing these breaks in eukaryotic cells are homologous recombination (HR) and non-homologous end-joining (NHEJ). However, a third group of still poorly characterized DSB repair pathways, collectively termed microhomology-mediated end-joining (MMEJ), relies on short homologies for the end-joining process. Here, we constructed GFP reporter assays to characterize and distingu...
Published on Apr 1, 2016in Vaccine3.27
Edward T. Mee9
Estimated H-index: 9
(NIBSC: National Institute for Biological Standards and Control),
Mark D. Preston14
Estimated H-index: 14
(NIBSC: National Institute for Biological Standards and Control)
+ 55 AuthorsGeorge Xu1
Estimated H-index: 1
Background Unbiased deep sequencing offers the potential for improved adventitious virus screening in vaccines and biotherapeutics. Successful implementation of such assays will require appropriate control materials to confirm assay performance and sensitivity.
Published on Sep 23, 2015
Ruthger W van Zwieten3
Estimated H-index: 3
,
Stefano Majocchi3
Estimated H-index: 3
+ 5 AuthorsNicolas Mermod30
Estimated H-index: 30
(UNIL: University of Lausanne)
A cornerstone of autologous cell therapy for Duchenne muscular dystrophy is the engineering of suitable cells to express dystrophin in a stable fashion upon differentiation to muscle fibers. Most viral transduction methods are typically restricted to the expression of truncated recombinant dystrophin derivatives and by the risk of insertional mutagenesis, while non-viral vectors often suffer from inefficient transfer, expression and/or silencing. Here we addressed such limitations by using plasm...
Published on Sep 2, 2015in Cell Cycle3.26
Kaja Kostyrko5
Estimated H-index: 5
,
Sandra Bosshard2
Estimated H-index: 2
+ 1 AuthorsNicolas Mermod30
Estimated H-index: 30
Eukaryotic cells respond to DNA breaks, especially double-stranded breaks (DSBs), by activating the DNA damage response (DDR), which encompasses DNA repair and cell cycle checkpoint signaling. The DNA damage signal is transmitted to the checkpoint machinery by a network of specialized DNA damage-recognizing and signal-transducing molecules. However, recent evidence suggests that DNA repair proteins themselves may also directly contribute to the checkpoint control. Here, we investigated the role ...
Published on Jul 1, 2015in Biotechnology Journal3.54
Niamh Harraghy6
Estimated H-index: 6
(UNIL: University of Lausanne),
David Calabrese4
Estimated H-index: 4
+ 4 AuthorsNicolas Mermod30
Estimated H-index: 30
(UNIL: University of Lausanne)
Abstract Successful generation of high producing cell lines requires the generation of cell clones expressing the recombinant protein at high levels and the characterization of the clones' ability to maintain stable expression levels. The use of cis-acting epigenetic regulatory elements that improve this otherwise long and uncertain process has revolutionized recombinant protein production. Here we review and discuss new insights into the molecular mode of action of the matrix attachment regions...
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