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Nicolas Mermod
University of Lausanne
132Publications
34H-index
4,645Citations
Publications 117
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#1Pierre-Olivier Duroy (UNIL: University of Lausanne)H-Index: 1
#2Sandra Bosshard (UNIL: University of Lausanne)H-Index: 2
Last.Nicolas Mermod (UNIL: University of Lausanne)H-Index: 34
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#1Flavien Buron (UNIL: University of Lausanne)
Last.Nicolas Mermod (UNIL: University of Lausanne)H-Index: 34
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#1Lionel O. Mavoungou (UNIL: University of Lausanne)H-Index: 1
#2Samuel Neuenschwander (Swiss Institute of Bioinformatics)H-Index: 12
Last.Nicolas Mermod (UNIL: University of Lausanne)H-Index: 34
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ABSTRACT Duchenne muscular dystrophy (DMD) is a lethal muscle-wasting disease caused by the lack of dystrophin in muscle fibers that is currently without curative treatment. Mesoangioblasts (MABs) are multipotent progenitor cells that can differentiate to a myogenic lineage and that can be used to express Dystrophin upon transplantation into muscles, in autologous gene therapy approaches. However, their fate in the muscle environment remains poorly characterized. Here, we investigated the differ...
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#1Sandra Bosshard (UNIL: University of Lausanne)H-Index: 2
#2Pierre-Olivier Duroy (UNIL: University of Lausanne)H-Index: 1
Last.Nicolas Mermod (UNIL: University of Lausanne)H-Index: 34
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Abstract CRISPR technologies greatly foster genome editing in mammalian cells through site-directed DNA double strand breaks (DSBs). However, precise editing outcomes, as mediated by homologous recombination (HR) repair, are typically infrequent and outnumbered by undesired genome alterations. By using knockdown and overexpression studies in Chinese hamster ovary (CHO) cells as well as characterizing repaired DNA junctions, we found that efficient HR-mediated genome editing depends on alternativ...
1 CitationsSource
#1Pavithra Iyer (UNIL: University of Lausanne)H-Index: 2
#2Lionel O. Mavoungou (UNIL: University of Lausanne)H-Index: 1
Last.Nicolas Mermod (UNIL: University of Lausanne)H-Index: 34
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Duchenne muscular dystrophy (DMD) is a lethal muscle-wasting disease currently without cure. We investigated the use of the PiggyBac transposon for full-length dystrophin expression in murine mesoangioblast (MABs) progenitor cells. DMD murine MABs were transfected with transposable expression vectors for full-length dystrophin and transplanted intramuscularly or intra-arterially into mdx/ SCID mice. Intra-arterial delivery indicated that the MABs could migrate to regenerating muscles to mediate ...
5 CitationsSource
#1Xuan Droz (UNIL: University of Lausanne)H-Index: 1
#2Niamh Harraghy (UNIL: University of Lausanne)H-Index: 6
Last.Nicolas Mermod (UNIL: University of Lausanne)H-Index: 34
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We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from th...
2 CitationsSource
#1Kaja Kostyrko (UNIL: University of Lausanne)H-Index: 6
#2Samuel Neuenschwander (Swiss Institute of Bioinformatics)H-Index: 12
Last.Nicolas Mermod (UNIL: University of Lausanne)H-Index: 34
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Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous recombination (HR) DNA repair pathways. However, a direct involvement of these pathways has remained unclear. Here, we show that the silencing of many HR factors enhanced plasmid concatemer formation and stable expression of the gene of interest...
7 CitationsSource
#1Kaja Kostyrko (UNIL: University of Lausanne)H-Index: 6
#2Nicolas Mermod (UNIL: University of Lausanne)H-Index: 34
DNA double stranded breaks (DSBs) are one of the most deleterious types of DNA lesions. The main pathways responsible for repairing these breaks in eukaryotic cells are homologous recombination (HR) and non-homologous end-joining (NHEJ). However, a third group of still poorly characterized DSB repair pathways, collectively termed microhomology-mediated end-joining (MMEJ), relies on short homologies for the end-joining process. Here, we constructed GFP reporter assays to characterize and distingu...
13 CitationsSource
#1Edward T. Mee (NIBSC: National Institute for Biological Standards and Control)H-Index: 9
#2Mark D. Preston (NIBSC: National Institute for Biological Standards and Control)H-Index: 15
Last.Heather D. MalickiH-Index: 1
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Background Unbiased deep sequencing offers the potential for improved adventitious virus screening in vaccines and biotherapeutics. Successful implementation of such assays will require appropriate control materials to confirm assay performance and sensitivity.
20 CitationsSource
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