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Didier Mazel
Pasteur Institute
126Publications
45H-index
6,766Citations
Publications 126
Newest
Jose Antonio Escudero7
Estimated H-index: 7
,
Aleksandra Nivina4
Estimated H-index: 4
(Paris Descartes University)
+ 3 AuthorsDidier Mazel45
Estimated H-index: 45
(Pasteur Institute)
Synthetic DNA design needs to harness the many information layers embedded in a DNA string. We previously developed the Evolutionary Landscape Painter (ELP), an algorithm that exploits the degeneracy of the code to increase protein evolvability. Here, we have used ELP to recode the integron integrase gene (intI1) in two alternative alleles. Although synonymous, both alleles yielded less IntI1 protein and were less active in recombination assays than intI1. We spliced the three alleles and mapped...
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Jose Antonio Escudero7
Estimated H-index: 7
,
Didier Mazel45
Estimated H-index: 45
Vibrio cholerae is one of the deadliest pathogens in the history of humankind. It is the causative agent of cholera, a disease characterized by a profuse and watery diarrhoea that still today causes 95.000 deaths worldwide every year. V. cholerae is a free living marine organism that interacts with and infects a variety of organisms, from amoeba to humans, including insects and crustaceans. The complexity of the lifestyle and ecology of V. cholerae suggests a high genetic and phenotypic plastici...
1 Citations Source Cite
2018 in BMC GenomicsIF: 3.73
Evelyne Krin22
Estimated H-index: 22
(Pasteur Institute),
Sebastian Aguilar Pierlé (Pasteur Institute)+ 10 AuthorsStéphane Cruveiller31
Estimated H-index: 31
(Centre national de la recherche scientifique)
Background The SOS response is an almost ubiquitous response of cells to genotoxic stresses. The full complement of genes in the SOS regulon for Vibrio species has only been addressed through bioinformatic analyses predicting LexA binding box consensus and in vitro validation. Here, we perform whole transcriptome sequencing from Vibrio cholerae treated with mitomycin C as an SOS inducer to characterize the SOS regulon and other pathways affected by this treatment.
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Florian Fournes1
Estimated H-index: 1
(Pasteur Institute),
Marie-Eve Val7
Estimated H-index: 7
(Pasteur Institute)
+ 1 AuthorsDidier Mazel45
Estimated H-index: 45
(Pasteur Institute)
Faithful vertical transmission of genetic information, especially of essential core genes, is a prerequisite for bacterial survival. Hence, replication of all the replicons is tightly controlled to ensure that all daughter cells get the same genome copy as their mother cell. Essential core genes are very often carried by the main chromosome. However they can occasionally be found on secondary chromosomes, recently renamed chromids. Chromids have evolved from non-essential megaplasmids, and furth...
1 Citations Source Cite
2018 in Nucleic Acids ResearchIF: 11.56
Francisco de Lemos Martins1
Estimated H-index: 1
(Pasteur Institute),
Florian Fournes1
Estimated H-index: 1
(Pasteur Institute)
+ 2 AuthorsMarie-Eve Val7
Estimated H-index: 7
(Pasteur Institute)
1 Citations Source Cite
2018
Jose Antonio Escudero7
Estimated H-index: 7
(Pasteur Institute),
Céline Loot7
Estimated H-index: 7
(Pasteur Institute),
Didier Mazel45
Estimated H-index: 45
(Pasteur Institute)
Integrons are genetic recombination platforms that enhance bacterial evolvability by enabling the acquisition and differential expression of new genes. These genes are embedded in mobile elements, named cassettes, which can be stockpiled to form a long array of adaptive functions whose expression is driven by the Pc promoter within the integron platform. Integron recombination is governed by the integrase, a distinct type of tyrosine recombinase. This protein can integrate, excise, and reshuffle...
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2018 in Nucleic Acids ResearchIF: 11.56
Ann Mukhortava1
Estimated H-index: 1
(Dresden University of Technology),
Matthias Pöge (Dresden University of Technology)+ 4 AuthorsMichael Schlierf9
Estimated H-index: 9
(Dresden University of Technology)
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2017 in BMC BiotechnologyIF: 2.60
Michael Jason Bland4
Estimated H-index: 4
(Pasteur Institute),
Magaly Ducos-Galand10
Estimated H-index: 10
(Pasteur Institute)
+ 1 AuthorsDidier Mazel45
Estimated H-index: 45
(Pasteur Institute)
Background Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable directional exchange and relocation of DNA sequences, making them valuable tools in these contexts. We have developed site-specific recombination tools that allow the direct selection of recombination events by embedding the attB site from each system w...
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