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Irina N. Chernysh
University of Pennsylvania
Molecular biologyThrombinFibrinogenPolymerizationFibrin
25Publications
9H-index
362Citations
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Publications 25
Newest
#1Irina N. Chernysh (UPenn: University of Pennsylvania)H-Index: 9
#2Chandrasekaran Nagaswami (UPenn: University of Pennsylvania)H-Index: 30
Last. John W. Weisel (UPenn: University of Pennsylvania)H-Index: 62
view all 9 authors...
Although arterial and venous thromboembolic disorders are among the most frequent causes of mortality and morbidity, there has been little description of how the composition of thrombi and emboli depends on their vascular origin and age. We quantified the structure and composition of arterial and venous thrombi and pulmonary emboli using high-resolution scanning electron microscopy. Arterial thrombi contained a surprisingly large amount of fibrin, in addition to platelets. The composition of pul...
3 CitationsSource
#1Irina N. Chernysh (UPenn: University of Pennsylvania)H-Index: 9
#2Russell Spiewak (UPenn: University of Pennsylvania)
Last. John W. Weisel (UPenn: University of Pennsylvania)H-Index: 62
view all 5 authors...
Abstract Pulmonary embolism occurs when blood flow to a part of the lungs is blocked by a venous thrombus that has traveled from the lower limbs. Little is known about the mechanical behavior of emboli under compressive forces from the surrounding musculature and blood pressure. We measured the stress-strain responses of human pulmonary emboli under cyclic compression, and showed that emboli exhibit a hysteretic stress-strain curve. The fibrin fibers and red blood cells (RBCs) are damaged during...
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#1Lilley Leong (Bayer)H-Index: 2
#2Irina N. Chernysh (UPenn: University of Pennsylvania)H-Index: 9
Last. John W. Weisel (UPenn: University of Pennsylvania)H-Index: 62
view all 10 authors...
Essentials Factor VIII (FVIII) replacement in hemophilia A corrects bleeding due to low thrombin generation. There is uncertainty about the minimal or trough level of FVIII needed for optimal treatment. FVIII improves physical properties of hemophilia clots, including stiffness and permeability. Fibrinogen and blood cells also stabilize hemophilia clots to decrease bleeding risk. Background Factor VIII (FVIII) replacement is standard of care for patients with hemophilia A (HemA); however, patien...
5 CitationsSource
#1Xiaojun Liang (UPenn: University of Pennsylvania)H-Index: 5
#2Irina N. Chernysh (UPenn: University of Pennsylvania)H-Index: 9
Last. John W. Weisel (UPenn: University of Pennsylvania)H-Index: 62
view all 4 authors...
Abstract Blood clots are required to stem bleeding and are subject to a variety of stresses, but they can also block blood vessels and cause heart attacks and ischemic strokes. We measured the compressive response of human platelet-poor plasma (PPP) clots, platelet-rich plasma (PRP) clots and whole blood clots and correlated these measurements with confocal and scanning electron microscopy to track changes in clot structure. Stress-strain curves revealed four characteristic regions, for compress...
4 CitationsSource
#1Brittany E. Bannish (UCO: University of Central Oklahoma)H-Index: 3
#2Irina N. Chernysh (UPenn: University of Pennsylvania)H-Index: 9
Last. John W. Weisel (UPenn: University of Pennsylvania)H-Index: 62
view all 5 authors...
Despite the common use of thrombolytic drugs, especially in stroke treatment, there are many conflicting studies on factors affecting fibrinolysis. Because of the complexity of the fibrinolytic system, mathematical models closely tied with experiments can be used to understand relationships within the system. When tPA is introduced at the clot or thrombus edge, lysis proceeds as a front. We developed a multiscale model of fibrinolysis that includes the main chemical reactions: the microscale mod...
8 CitationsSource
#1Lilley Leong (Bayer)H-Index: 2
#2Irina N. Chernysh (UPenn: University of Pennsylvania)H-Index: 9
Last. John W. Weisel (UPenn: University of Pennsylvania)H-Index: 62
view all 11 authors...
Patients with severe factor VIII (FVIII) deficiency (hemophilia A [HemA]) develop neutralizing antibodies (inhibitors) against FVIII in up to ~30% of cases. For HemA patients with inhibitors, activated recombinant factor VII (rFVIIa) is a treatment option. High levels of rFVIIa are required for treating HemA patients with inhibitors to induce direct activation of factor X on the surface of activated platelets via a tissue factor (TF)-independent mechanism (Hoffman M, Monroe DM. Thromb Res. 2010;...
#1Claire S. WhyteH-Index: 6
#2Irina N. Chernysh (UPenn: University of Pennsylvania)H-Index: 9
Last. Nicola J. MutchH-Index: 8
view all 7 authors...
Polyphosphate (polyP) binds to fibrin(ogen) and alters fibrin structure, generating a heterogeneous network composed of ‘knots’ interspersed by large pores. Here we show platelet-derived polyP elicits similar structural changes in fibrin and examine the mechanism by which polyP alters fibrin structure. Polymerisation of fibrinogen with thrombin and CaCl2 was studied using spinning disk confocal (SDC) microscopy. PolyP delayed fibrin polymerisation generating shorter protofibrils emanating from a...
6 CitationsSource
#1Irina N. ChernyshH-Index: 9
Last. John W. WeiselH-Index: 2
view all 3 authors...
#1Nafiseh Badiei (Swansea University)H-Index: 6
#2A.M. SowedanH-Index: 1
Last. Karl HawkinsH-Index: 18
view all 13 authors...
Incipient clot formation in whole blood and fibrin gels was studied by the rheometric techniques of controlled stress parallel superposition (CSPS) and small amplitude oscillatory shear (SAOS). The effects of unidirectional shear stress on incipient clot microstructure, formation kinetics and elasticity are reported in terms of the fractal dimension (df) of the fibrin network, the gel network formation time (TGP) and the shear elastic modulus, respectively. The results of this first haemorheolog...
7 CitationsSource
Our objective was to identify mechanisms for the ultrasound-enhanced acceleration of tissue-type plasminogen activator (t-PA)-induced fibrinolysis of clots. Measurements of turbidity as a function of time, used to characterize quantitatively the effects of ultrasound, showed that the ultrasound pulse-repetition frequency affected clot lysis times, but there were no thermal effects. Ultrasound in the absence of t-PA produced a slight but consistent decrease in turbidity, suggesting a decrease in ...
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