Claudio Tavares Sacchi
Instituto Adolfo Lutz
VirologyMeningococcal diseaseNeisseria meningitidisBiologyMicrobiology
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Publications 90
#1Carla Adriana Dos Santos (Instituto Adolfo Lutz)
#2Marcos Paulo Vieira Cunha (USP: University of São Paulo)H-Index: 6
Last. Monique Ribeiro Tiba-Casas (Instituto Adolfo Lutz)H-Index: 2
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#1Laís Calissi Brisolla Tavares (USP: University of São Paulo)H-Index: 1
#2Marcos Paulo Vieira Cunha (USP: University of São Paulo)H-Index: 6
Last. Alessandro Lia Mondelli (UNESP: Sao Paulo State University)H-Index: 7
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Acinetobacter baumannii is the main species of the Acinetobacter genus; however, non-baumannii Acinetobacter (NBA) species causing infections have been described for the past years, as well as antimicrobial resistance. In this study, we describe the occurrence of two multidrug-resistant (MDR) IMP-1-producing Acinetobacter bereziniae isolates recovered from bloodstream infections in different patients but in the same intensive care unit among 134 carbapenem-resistant Acinetobacter screened. Antim...
#1Juliana Mariotti Guerra (Instituto Adolfo Lutz)H-Index: 7
#2Natália Coelho Couto de Azevedo Fernandes (USP: University of São Paulo)
Last. Claudio Tavares Sacchi (Instituto Adolfo Lutz)H-Index: 25
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After the sudden death of eleven captives marmosets in a rehabilitation center of wildlife in Sao Paulo, Brazil, histological and microbiological study was conducted. Liver, spleen, intestine, central nervous system, lung, thymus, stomach, testicle tissues were analyzed by light microscopy and microbial cultures were conducted. Environmental cultures were also performed. Prophylactic antimicrobial therapy, restricted access to marmosets' cages with dedicated staff, and additional sanitization of...
#1Arijana BorasH-Index: 3
#2T JerenH-Index: 3
Last. Claudio Tavares SacchiH-Index: 25
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#1Monique Ribeiro Tiba-Casas (Instituto Adolfo Lutz)H-Index: 2
#2Claudio Tavares Sacchi (Instituto Adolfo Lutz)H-Index: 25
Last. Carlos Henrique Camargo (Instituto Adolfo Lutz)H-Index: 5
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Abstract Background The primary method of molecular subtyping for the identification and investigation of outbreaks has been pulsed-field gel electrophoresis (PFGE). In some cases, this technique has not been able to show discrimination between the unrelated strains that can be achieved by whole genome sequencing (WGS). Methods The aim of this study was to determine the strengths and drawbacks of WGS using different analytic approaches compared to traditional typing method, PFGE, for retrospecti...
#1Mahamoudou Ouattara (CDC: Centers for Disease Control and Prevention)H-Index: 2
#2Melissa Whaley (CDC: Centers for Disease Control and Prevention)H-Index: 13
Last. Xin Wang (CDC: Centers for Disease Control and Prevention)H-Index: 19
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Abstract This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput.
#1Melissa WhaleyH-Index: 13
#2Laurel T. JenkinsH-Index: 3
Last. Xin WangH-Index: 16
view all 8 authors...
Detection of Neisseria meningitidis has become less time- and resource-intensive with a monoplex direct real-time PCR (drt-PCR) to amplify genes from clinical specimens without DNA extraction. To further improve efficiency, we evaluated two triplex drt-PCR assays for the detection of meningococcal serogroups AWX and BCY. The sensitivity and specificity of the triplex assays were assessed using 228 cerebrospinal fluid (CSF) specimens from meningitis patients and compared to the monoplex for six s...