Meera Shanmuganathan
McMaster University
Publications 9
#1Meera Shanmuganathan (McMaster University)H-Index: 4
#2Sabrina Macklai (McMaster University)
Last.Philip Britz-McKibbin (McMaster University)H-Index: 26
view all 8 authors...
New analytical methods are urgently needed to enable high-throughput, yet comprehensive drug screening, given an alarming opioid and prescription drug crisis in public health. Conventional urine drug testing based on a two-tier immunoassay screen followed by a gas chromatography-tandem mass spectrometry (GC-MS/MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) method are expensive and prone to bias while being limited to targeted panels of known drugs of abuse (DoA). Herein, we out...
#1Biban Gill (McMaster University)H-Index: 1
#2Alicia Mell (McMaster University)
Last.Philip Britz-McKibbin (McMaster University)H-Index: 26
view all 8 authors...
Urinary 1-hydroxypyrene (OH-Pyr) is widely used for biomonitoring human exposures to polycyclic aromatic hydrocarbons (PAHs) from air pollution and tobacco smoke. However, there have been few rigorous validation studies reported to ensure reliable OH-Pyr determination for occupational health and risk assessment. Herein, we report an inter-laboratory method comparison for urinary OH-Pyr when using gas chromatography-high-resolution mass spectrometry (GC-HRMS) and liquid chromatography-tandem mass...
Management of phenylketonuria (PKU) requires lifelong restriction of phenylalanine (Phe) intake using specialized medical foods to prevent neurocognitive impairment in affected patients. However, dietary adherence is challenging to maintain while ensuring adequate nutrition, which can lead to sub-optimal clinical outcomes. Metabolomics offers a systematic approach to identify new biomarkers of disease progression in PKU when using urine as a surrogate for blood specimens that is more accurate th...
#1Christian Wenz (Agilent Technologies)H-Index: 8
#2Coral BarbasH-Index: 43
Last.Meera Shanmuganathan (McMaster University)H-Index: 4
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A collaborative study on the robustness and portability of a capillary electrophoresis-mass spectrometry method for peptide mapping was performed by an international team, consisting of 13 independent laboratories from academia and industry. All participants used the same batch of samples, reagents and coated capillaries to run their assays, whereas they utilized the capillary electrophoresis-mass spectrometry equipment available in their laboratories. The equipment used varied in model, type an...
#1Meera Shanmuganathan (McMaster University)H-Index: 4
#2Philip Britz-McKibbin (McMaster University)H-Index: 26
Abstract High quality assays are needed in drug discovery to reduce the high attrition rate of lead compounds during primary screening. Capillary electrophoresis (CE) represents a versatile micro-separation technique for resolution of enzyme-catalyzed reactions, including substrate(s), product(s), cofactor(s) and their stereoisomers, which is needed for reliable characterization of biomolecular interactions in free solution. This review article provides a critical overview of new advances in CE ...