Ryo Oiwa
Central Institute for Experimental Animals
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Publications 6
#1Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
#2Ryo Oiwa (Central Institute for Experimental Animals)H-Index: 5
Last. Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
view all 26 authors...
Recent advances in genome editing have facilitated the generation of nonhuman primate (NHP) models, with potential to unmask the complex biology of human disease not revealed by rodent models. However, their broader use is hindered by the challenges associated with generation of adult NHP models as well as the cost of their production. Here, we describe the generation of a marmoset model of severe combined immunodeficiency (SCID). This study optimized zinc-finger nucleases (ZFNs) and transcripti...
55 CitationsSource
#1Daisuke H. TanakaH-Index: 10
#2Ryo Oiwa (Central Institute for Experimental Animals)H-Index: 5
Last. Kazunori NakajimaH-Index: 50
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The establishment of the mammalian neocortex is often explained phylogenetically by an evolutionary change in the pallial neuronal progenitors of excitatory projection neurons. It remains unclear, however, whether and how the evolutionary change in inhibitory interneurons, which originate outside the neocortex, has been involved in the establishment of the neocortex. In this study, we transplanted chicken, turtle, mouse, and marmoset medial ganglionic eminence (MGE) cells into the embryonic mous...
23 CitationsSource
#1Ikuo Tomioka (Central Institute for Experimental Animals)H-Index: 11
#2Takuji Maeda (Central Institute for Experimental Animals)H-Index: 5
Last. Hideyuki Okano (Keio: Keio University)H-Index: 112
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Although embryonic stem (ES) cell–like induced pluripotent stem (iPS) cells have potential therapeutic applications in humans, they are also useful for creating genetically modified human disease models in nonhuman primates. In this study, we generated common marmoset iPS cells from fetal liver cells via the retrovirus-mediated introduction of six human transcription factors: Oct-3/4, Sox2, Klf4, c-Myc, Nanog, and Lin28. Four to five weeks after introduction, several colonies resembling marmoset...
85 CitationsSource
#1Daisuke Tanaka Harada (Keio: Keio University)
#2Ryo OiwaH-Index: 5
Last. Kazunori Nakajima (Keio: Keio University)H-Index: 50
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#1Yusuke Sotomaru (Hiroshima University)H-Index: 18
#2Reiko Hirakawa (Central Institute for Experimental Animals)H-Index: 2
Last. Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
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Abstract The somatic cell nuclear transfer technique has been applied to various mammals to produce cloned animals; however, a standardized method is not applicable to all species. We aimed here to develop optimum procedures for somatic cell cloning in nonhuman primates, using common marmosets. First, we confirmed that parthenogenetic activation of in vitro matured oocytes was successfully induced by electrical stimulation (three cycles of 150 V/mm, 50 μsec × 2, 20 min intervals), and this condi...
7 CitationsSource
#1Erika Sasaki (Central Institute for Experimental Animals)H-Index: 21
#2Hiroshi Suemizu (Central Institute for Experimental Animals)H-Index: 21
Last. Tatsuji Nomura (Central Institute for Experimental Animals)H-Index: 35
view all 23 authors...
The common marmoset (Callithrix jacchus) is increasingly attractive for use as a non-human primate animal model in biomedical research. It has a relatively high reproduction rate for a primate, making it potentially suitable for transgenic modification. Although several attempts have been made to produce non-human transgenic primates, transgene expression in the somatic tissues of live infants has not been demonstrated by objective analyses such as polymerase chain reaction with reverse transcri...
453 CitationsSource