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Graham H. Coombs
Strathclyde Institute of Pharmacy and Biomedical Sciences
290Publications
61H-index
19kCitations
Publications 290
Newest
#1Gareth D. Westrop (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 12
#2Lijie WangH-Index: 3
Last.Graham H. Coombs (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 61
view all 7 authors...
Trichomonas vaginalis and Tritrichomonas foetus are pathogens that parasitise, respectively, human and bovine urogenital tracts causing disease. Using LC-MS, reference metabolomic profiles were obtained for both species and stable isotope labelling with D-[U-13C6] glucose was used to analyse central carbon metabolism. This facilitated a comparison of the metabolic pathways of T. vaginalis and T. foetus, extending earlier targeted biochemical studies. 43 metabolites, whose identities were confirm...
#1Karen McLuskey (Wellcome Trust)H-Index: 14
#2Jaspreet Singh Grewal (Wellcome Trust)H-Index: 2
Last.Jeremy C. Mottram (Wellcome Trust)H-Index: 61
view all 10 authors...
Clan CD cysteine peptidases, a structurally related group of peptidases that include mammalian caspases, exhibit a wide range of important functions, along with a variety of specificities and activation mechanisms. However, for the clostripain family (denoted C11), little is currently known. Here, we describe the first crystal structure of a C11 protein from the human gut bacterium, Parabacteroides merdae (PmC11), determined to 1.7 A resolution. PmC11 is a monomeric cysteine peptidase that compr...
#1Jaspreet Singh Grewal (Ebor: University of York)H-Index: 2
#2Karen McLuskey (Wellcome Trust)H-Index: 14
Last.Graham H. Coombs (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 61
view all 10 authors...
The structure of a C11 peptidase PmC11 from the gut bacterium, Parabacteroides merdae, has recently been determined, enabling the identification and characterization of a C11 orthologue, PNT1, in the parasitic protozoon Trypanosoma brucei. A phylogenetic analysis identified PmC11 orthologues in bacteria, archaea, Chromerids, Coccidia, and Kinetoplastida, the latter being the most divergent. A primary sequence alignment of PNT1 with clostripain and PmC11 revealed the position of the characteristi...
#1C. D. Shaw (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 2
#2J. Lonchamp (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 1
Last.Suman RijalH-Index: 40
view all 10 authors...
n this study, we followed the genomic, lipidomic and metabolomic changes associated with the selection of miltefosine (MIL) resistance in two clinically derived Leishmania donovani strains with different inherent resistance to antimonial drugs (antimony sensitive strain Sb-S; and antimony resistant Sb-R). MIL-R was easily induced in both strains using the promastigote-stage, but a significant increase in MIL-R in the intracellular amastigote compared to the corresponding wild-type did not occur ...
#1Daniel J. Klionsky (UM: University of Michigan)H-Index: 106
#2Kotb Abdelmohsen (NIH: National Institutes of Health)H-Index: 46
Last.Khosrow Adeli (U of T: University of Toronto)H-Index: 54
view all 10 authors...
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues...
#1C. D. ShawH-Index: 2
#2J. LonchampH-Index: 1
Last.Suman RijalH-Index: 40
view all 10 authors...
#1Daniel J. Klionsky (UM: University of Michigan)H-Index: 106
#2Kotb Abdelmohsen (NIH: National Institutes of Health)H-Index: 46
Last.Khosrow Adeli (U of T: University of Toronto)H-Index: 54
view all 10 authors...
Author(s): Klionsky, DJ; Abdelmohsen, K; Abe, A; Abedin, MJ; Abeliovich, H; Arozena, AA; Adachi, H; Adams, CM; Adams, PD; Adeli, K; Adhihetty, PJ; Adler, SG; Agam, G; Agarwal, R; Aghi, MK; Agnello, M; Agostinis, P; Aguilar, PV; Aguirre-Ghiso, J; Airoldi, EM; Ait-Si-Ali, S; Akematsu, T; Akporiaye, ET; Al-Rubeai, M; Albaiceta, GM; Albanese, C; Albani, D; Albert, ML; Aldudo, J; Algul, H; Alirezaei, M; Alloza, I; Almasan, A; Almonte-Beceril, M; Alnemri, ES; Alonso, C; Altan-Bonnet, N; Altieri, DC; A...
#1C. D. ShawH-Index: 2
#2J. LonchampH-Index: 1
Last.Suman RijalH-Index: 40
view all 10 authors...
#1Gareth D. Westrop (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 12
#2Roderick A. M. Williams (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 15
Last.Graham H. Coombs (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 61
view all 7 authors...
Comparative genomic analyses of Leishmania species have revealed relatively minor heterogeneity amongst recognised housekeeping genes and yet the species cause distinct infections and pathogenesis in their mammalian hosts. To gain greater information on the biochemical variation between species, and insights into possible metabolic mechanisms underpinning visceral and cutaneous leishmaniasis, we have undertaken in this study a comparative analysis of the metabolomes of promastigotes of L. donova...
#1Benjamin Cull (Glas.: University of Glasgow)H-Index: 3
#2Joseane Lima Prado Godinho (UFRJ: Federal University of Rio de Janeiro)H-Index: 6
Last.Jeremy C. Mottram (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 61
view all 8 authors...
Autophagy is a central process behind the cellular remodeling that occurs during differentiation of Leishmania, yet the cargo of the protozoan parasite's autophagosome is unknown. We have identified glycosomes, peroxisome-like organelles that uniquely compartmentalize glycolytic and other metabolic enzymes in Leishmania and other kinetoplastid parasitic protozoa, as autophagosome cargo. It has been proposed that the number of glycosomes and their content change during the Leishmania life cycle a...
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