Match!
Graham H. Coombs
Strathclyde Institute of Pharmacy and Biomedical Sciences
Molecular biologyLeishmaniaLeishmania mexicanaBiochemistryBiology
298Publications
61H-index
19.5kCitations
What is this?
Publications 297
Newest
#1C. D. Shaw (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 2
#2Hideo ImamuraH-Index: 17
Last. Katharine C. Carter (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 23
view all 12 authors...
Understanding the mechanism(s) underpinning drug resistance could lead to novel treatments to reverse the increased tolerance of a pathogen. In this study paromomycin (PMM) resistance (PMM-R) was induced in three Nepalese clinical strains of L. donovani, with different inherent susceptibility to antimony drugs (Sb), by step-wise exposure of promastigotes to PMM. Exposure to PMM resulted in the production of mixed populations of parasites even though a single cloned population was used at the sta...
1 CitationsSource
#1Gareth D. Westrop (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 19
#2Lijie WangH-Index: 4
Last. Graham H. Coombs (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 61
view all 7 authors...
Trichomonas vaginalis and Tritrichomonas foetus are pathogens that parasitise, respectively, human and bovine urogenital tracts causing disease. Using LC-MS, reference metabolomic profiles were obtained for both species and stable isotope labelling with D-[U-13C6] glucose was used to analyse central carbon metabolism. This facilitated a comparison of the metabolic pathways of T. vaginalis and T. foetus, extending earlier targeted biochemical studies. 43 metabolites, whose identities were confirm...
4 CitationsSource
#1Karen McLuskey (Wellcome Trust)H-Index: 14
#2Jaspreet Singh Grewal (Wellcome Trust)H-Index: 2
Last. Jeremy C. Mottram (Wellcome Trust)H-Index: 62
view all 10 authors...
Clan CD cysteine peptidases, a structurally related group of peptidases that include mammalian caspases, exhibit a wide range of important functions, along with a variety of specificities and activation mechanisms. However, for the clostripain family (denoted C11), little is currently known. Here, we describe the first crystal structure of a C11 protein from the human gut bacterium, Parabacteroides merdae (PmC11), determined to 1.7 A resolution. PmC11 is a monomeric cysteine peptidase that compr...
8 CitationsSource
#1Jaspreet Singh Grewal (Ebor: University of York)H-Index: 2
#2Karen McLuskey (Wellcome Trust)H-Index: 14
Last. Jeremy C. Mottram (Ebor: University of York)H-Index: 62
view all 12 authors...
The structure of a C11 peptidase PmC11 from the gut bacterium, Parabacteroides merdae, has recently been determined, enabling the identification and characterization of a C11 orthologue, PNT1, in the parasitic protozoon Trypanosoma brucei. A phylogenetic analysis identified PmC11 orthologues in bacteria, archaea, Chromerids, Coccidia, and Kinetoplastida, the latter being the most divergent. A primary sequence alignment of PNT1 with clostripain and PmC11 revealed the position of the characteristi...
5 CitationsSource
#1C. D. Shaw (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 2
#2Julien Lonchamp (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 5
Last. Katharine C. Carter (Strathclyde Institute of Pharmacy and Biomedical Sciences)H-Index: 23
view all 14 authors...
n this study, we followed the genomic, lipidomic and metabolomic changes associated with the selection of miltefosine (MIL) resistance in two clinically derived Leishmania donovani strains with different inherent resistance to antimonial drugs (antimony sensitive strain Sb-S; and antimony resistant Sb-R). MIL-R was easily induced in both strains using the promastigote-stage, but a significant increase in MIL-R in the intracellular amastigote compared to the corresponding wild-type did not occur ...
27 CitationsSource
#1Daniel J. Klionsky (UM: University of Michigan)H-Index: 113
#2Kotb Abdelmohsen (NIH: National Institutes of Health)H-Index: 48
Last. Susu M. Zughaier (Emory University)H-Index: 24
view all 2461 authors...
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues...
1,669 CitationsSource
#1C. D. ShawH-Index: 2
#2Julien LonchampH-Index: 5
Last. Katharine C. CarterH-Index: 23
view all 14 authors...
#1Dj KlionskyH-Index: 1
#2Kotb AbdelmohsenH-Index: 48
Last. Sm ZughaierH-Index: 1
view all 2385 authors...
28 CitationsSource
#1C. D. ShawH-Index: 2
#2Julien LonchampH-Index: 5
Last. Katharine C. CarterH-Index: 23
view all 14 authors...
#1Daniel J. Klionsky (UM: University of Michigan)H-Index: 113
#2Kotb Abdelmohsen (NIH: National Institutes of Health)H-Index: 48
Last. Susu M. Zughaier (Emory University)H-Index: 24
view all 2461 authors...
Author(s): Klionsky, DJ; Abdelmohsen, K; Abe, A; Abedin, MJ; Abeliovich, H; Arozena, AA; Adachi, H; Adams, CM; Adams, PD; Adeli, K; Adhihetty, PJ; Adler, SG; Agam, G; Agarwal, R; Aghi, MK; Agnello, M; Agostinis, P; Aguilar, PV; Aguirre-Ghiso, J; Airoldi, EM; Ait-Si-Ali, S; Akematsu, T; Akporiaye, ET; Al-Rubeai, M; Albaiceta, GM; Albanese, C; Albani, D; Albert, ML; Aldudo, J; Algul, H; Alirezaei, M; Alloza, I; Almasan, A; Almonte-Beceril, M; Alnemri, ES; Alonso, C; Altan-Bonnet, N; Altieri, DC; A...
17 CitationsSource
12345678910