Hideyuki Okano
Keio University
Neural stem cellMolecular biologyStem cellBiologyCell biology
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Publications 1245
#1Satoru MorimotoH-Index: 6
#2Mitsuru IshikawaH-Index: 7
Last. Hideyuki OkanoH-Index: 112
view all 11 authors...
Amyotrophic lateral sclerosis and Parkinsonism-dementia complex (ALS/PDC) is a unique endemic neurodegenerative disease, with high-incidence foci in Kii Peninsula, Japan. To gather new insights into the pathological mechanisms underlying Kii ALS/PDC, we performed transcriptome analyses of patient brains. We prepared frozen brains from three individuals without neurodegenerative diseases, three patients with Alzheimer’s disease, and 21 patients with Kii ALS/PDC, and then acquired microarray data ...
#1Taro Tsujimura (Keio: Keio University)H-Index: 1
#2Osamu Takase (Keio: Keio University)H-Index: 3
Last. Keiichi Hishikawa (Keio: Keio University)H-Index: 33
view all 10 authors...
While regulation of gene-enhancer interaction is intensively studied, its application remains limited. Here, we reconstituted arrays of CTCF-binding sites and devised a synthetic topological insulator with tetO for chromatin-engineering (STITCH). By coupling STITCH with tetR linked to the KRAB domain to induce heterochromatin and disable the insulation, we developed a drug-inducible system to control gene activation by enhancers. In human induced pluripotent stem cells, STITCH inserted between M...
#1Alexander Woodward (Woodward, Inc.)H-Index: 1
#2Rui GongH-Index: 3
Last. Tetsuo YamamoriH-Index: 26
view all 11 authors...
We describe our connectomics pipeline for processing anterograde tracer injection data for the brain of the common marmoset (Callithrix jacchus). Brain sections were imaged using a batch slide scanner (NanoZoomer 2.0-HT) and we used artificial intelligence to precisely segment the tracer signal from the background in the fluorescence images. The shape of each brain was reconstructed by reference to a block-face and all data were mapped into a common 3D brain space with atlas and 2D cortical flat...
1 CitationsSource
#1Yuki Oiwa (Kumamoto University)
#2Kaori Oka (Kumamoto University)
Last. Atsushi Shimizu (Iwate Medical University)H-Index: 12
view all 15 authors...
The naked mole-rat (NMR) is a poikilothermic mammal that forms eusocial colonies consisting of one breeding queen, several breeding kings, and subordinates. Despite their poikilothermic feature, NMRs possess brown adipose tissue (BAT), which in homeothermic mammals induces thermogenesis in cold environments. However, NMR-BAT thermogenic potential is controversial, and its physiological roles are unknown. Here, we show that NMR-BAT has beta-3 adrenergic receptor (ADRB3)-dependent thermogenic pote...
#1Carlos E. Gutierrez (OIST: Okinawa Institute of Science and Technology)H-Index: 1
#2Henrik SkibbeH-Index: 8
Last. Hideyuki OkanoH-Index: 112
view all 14 authors...
Diffusion-weighted magnetic resonance imaging (dMRI) allows non-invasive investigation of whole-brain connectivity, which can potentially help to reveal the brain's global network architecture and abnormalities involved in neurological and mental disorders. However, the reliability of connection inferences from dMRI-based fiber tracking is still debated, due to low sensitivity, dominance of false positives, and inaccurate and incomplete reconstruction of long-range connections. Furthermore, para...
#1Seiji Shiozawa (Keio: Keio University)H-Index: 5
#2Mayutaka Nakajima (Keio: Keio University)H-Index: 1
Last. Yohei SasagawaH-Index: 5
view all 13 authors...
Mammalian pluripotent stem cells are thought to exist in two states: naive and primed states. Generally, unlike those in rodents, pluripotent stem cells in primates including humans are regarded as being in the primed pluripotent state. Recently, several groups reported the existence of naive pluripotent stem cells in humans. In this study, we report the conversion of primed state embryonic stem cells from common marmoset, a New World monkey, to the naive state by using transgenes. The cells sho...
#1Narihito NagoshiH-Index: 21
#2Osahiko Tsuji (Keio: Keio University)H-Index: 19
Last. Hideyuki OkanoH-Index: 112
view all 11 authors...
Spinal cord injury (SCI) is an abrupt traumatic injury that leads to permanent functional loss, and no practical treatment is available. We have developed pharmaceutical recombinant human hepatocyte growth factor (KP-100), and its efficacy for SCI has been verified using animal models. The purpose of this study was to evaluate the safety and efficacy of intrathecal KP-100 administration for SCI patients in the acute phase. This investigation was a multicenter, randomized, double-blind study. Sub...
#1Makoto Hosoya (Keio: Keio University)H-Index: 5
#2Masato Fujioka (Keio: Keio University)H-Index: 12
Last. Kaoru Ogawa (Keio: Keio University)H-Index: 29
view all 5 authors...
Cochlear development is a complex process with precise spatiotemporal patterns. A detailed understanding of this process is important for studies of congenital hearing loss and regenerative medicine. However, much of our understanding of cochlear development is based on rodent models. Animal models that bridge the gap between humans and rodents are needed. In this study, we investigated the development of hearing organs in a small New World monkey species, the common marmoset (Callithrix jacchus...
#1Masato Yugami (Takeda Pharmaceutical Company)H-Index: 1
#2Hideyuki Okano (Keio: Keio University)H-Index: 112
Last. Masato Yano (Niigata University)H-Index: 10
view all 4 authors...
RNA-binding proteins (RBPs) control many types of post-transcriptional regulation, including mRNA splicing, mRNA stability, and translational efficiency, by directly binding to their target RNAs and their mutation and dysfunction are often associated with several human neurological diseases and tumorigenesis. Crosslinking immunoprecipitation (CLIP), coupled with high-throughput sequencing (HITS-CLIP), is a powerful technique for investigating the molecular mechanisms underlying disease pathogene...
#1Kahori Shiba-Fukushima (Juntendo University)H-Index: 11
#2Tsuyoshi Inoshita (Juntendo University)H-Index: 9
Last. Nobutaka Hattori (Juntendo University)H-Index: 76
view all 9 authors...
Summary Early-onset Parkinson’s disease-associated PINK1-Parkin signaling maintains mitochondrial health. Therapeutic approaches for enhancing PINK1-Parkin signaling present a potential strategy for treating various diseases caused by mitochondrial dysfunction. We report two chemical enhancers of PINK1-Parkin signaling, identified using a robust cell-based high-throughput screening system. These small molecules, T0466 and T0467, activate Parkin mitochondrial translocation in dopaminergic neurons...