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Hideyuki Okano
Keio University
1,186Publications
110H-index
47kCitations
Publications 1186
Newest
#1Joanes GrandjeanH-Index: 11
Last.Natalia GassH-Index: 10
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Abstract Preclinical applications of resting-state functional magnetic resonance imaging (rsfMRI) offer the possibility to non-invasively probe whole-brain network dynamics and to investigate the determinants of altered network signatures observed in human studies. Mouse rsfMRI has been increasingly adopted by numerous laboratories worldwide. Here we describe a multi-centre comparison of 17 mouse rsfMRI datasets via a common image processing and analysis pipeline. Despite prominent cross-laborat...
#1Yutaka Hoshino (Keio: Keio University)
#2Kenji Nishide (Keio: Keio University)
Last.Masaya Nakamura (Keio: Keio University)H-Index: 52
view all 11 authors...
Treatment options for chronic spinal cord injury (SCI) remain limited due to unfavourable changes in the microenvironment. Gene therapy can overcome these barriers through continuous delivery of therapeutic gene products to the target tissue. In particular, adeno-associated virus (AAV) vectors are potential candidates for use in chronic SCI, considering their safety and stable gene expression in vivo. Given that different AAV serotypes display different cellular tropisms, it is extremely importa...
#1Johannes Lohmeier (Charité)
#2Takaaki KanekoH-Index: 3
Last.Hideyuki Okano (Keio: Keio University)H-Index: 110
view all 5 authors...
We proposed a generic template-derived approach for (semi-) automated brain extraction in animal MRI studies and evaluated our implementation with different animal models (macaque, marmoset, rodent) and MRI protocols (T1, T2). While conventional MR-neuroimaging studies perform brain extraction as an initial step priming subsequent image-registration from subject to template, our proposed approach propagates an anatomical template to (whole-head) individual subjects in reverse order, which is cha...
#1Ryota Tamura (Keio: Keio University)H-Index: 4
#2Hiroyuki Miyoshi (Keio: Keio University)H-Index: 47
Last.Tetsuo Maruyama (Keio: Keio University)H-Index: 28
view all 15 authors...
Glioblastoma exhibits phenotypic and genetic heterogeneity, aggressive invasiveness, therapeutic resistance, and tumor recurrence, which can be explained by the existence of glioma stem cells (GSCs). In this study, we visualized the spatiotemporal dynamics of invasion of human GSCs in an orthotopic xenograft mouse model using time-lapse imaging of organotypic brain slice cultures and three-dimensional imaging of optically cleared whole brains. GSCs implanted in the striatum exhibited directional...
#1Narihito Nagoshi (Keio: Keio University)H-Index: 18
#2Osahiko Tsuji (Keio: Keio University)H-Index: 18
Last.Hideyuki Okano (Keio: Keio University)H-Index: 110
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Abstract For the past few decades, spinal cord injury (SCI) has been believed to be an incurable traumatic condition, but with recent developments in stem cell biology, the field of regenerative medicine has gained hopeful momentum in the development of a treatment for this challenging pathology. Among the treatment candidates, transplantation of neural precursor cells has gained remarkable attention as a reasonable therapeutic intervention to replace the damaged central nervous system cells and...
#1Chizuru Iwasawa (Hoshi University)H-Index: 2
#2Naoko Kuzumaki (Hoshi University)H-Index: 18
Last.Minoru Narita (Hoshi University)H-Index: 39
view all 8 authors...
Parkinson’s disease (PD) is associated with both motor and non-motor symptoms, including constipation, sensory neuropathy, depression, dementia and sleep disorder. Somatostatin (SST) is considered to be a modulator of GABAergic inhibitory transmission, and its levels are reduced in cerebrospinal fluid of PD patients. In the present study, we evaluated the changes in the expression of SST in GABAergic neurons derived from induced pluripotent stem cells (iPSCs) of PD patients. Neural cells were co...
#1Satoru Morimoto (Keio: Keio University)H-Index: 5
#2Shin Ichi Takahashi (Keio: Keio University)H-Index: 15
Last.Jin Nakahara (Keio: Keio University)H-Index: 14
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Abstract Introduction Amyotrophic lateral sclerosis (ALS) is an intractable and incurable neurological disease. It is a progressive disease characterized by muscle atrophy and weakness caused by selective vulnerability of upper and lower motor neurons. In disease research, it has been common to use mouse models carrying mutations in responsible genes for familial ALS as pathological models of ALS. However, there is no model that has reproduced the actual conditions of human spinal cord pathology...
#1Sho Yoshimatsu (Keio: Keio University)H-Index: 1
#2Junko Okahara (Central Institute for Experimental Animals)H-Index: 5
Last.Hideyuki Okano (Keio: Keio University)H-Index: 110
view all 9 authors...
Genome editing technology greatly facilitates the genetic modification of various cells and animals. The common marmoset (Callithrix jacchus), a small non-human primate which exhibits high reproductive efficiency, is a widely used animal model in biomedical research. Developing genome editing techniques in the common marmoset will further enhance its utility. Here, we report the successful establishment of a knock-in (KI) method for marmoset embryonic stem cells (ESCs), which is based on the CRI...
#1Naoto Muraoka (Keio: Keio University)H-Index: 8
#2Kaori Nara (Keio: Keio University)H-Index: 1
Last.Hidenori Tani (Keio: Keio University)H-Index: 2
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Direct cardiac reprogramming from fibroblasts can be a promising approach for disease modeling, drug screening, and cardiac regeneration in pediatric and adult patients. However, postnatal and adult fibroblasts are less efficient for reprogramming compared with embryonic fibroblasts, and barriers to cardiac reprogramming associated with aging remain undetermined. In this study, we screened 8400 chemical compounds and found that diclofenac sodium (diclofenac), a non-steroidal anti-inflammatory dr...
#1Wakako Kumita (Central Institute for Experimental Animals)H-Index: 2
#2Kenya Sato (Central Institute for Experimental Animals)H-Index: 3
Last.Yasubumi Sakakibara (Keio: Keio University)H-Index: 29
view all 13 authors...
Genetically modified nonhuman primates (NHP) are useful models for biomedical research. Gene editing technologies have enabled production of target-gene knock-out (KO) NHP models. Target-gene-KO/knock-in (KI) efficiency of CRISPR/Cas9 has not been extensively investigated in marmosets. In this study, optimum conditions for target gene modification efficacies of CRISPR/mRNA and CRISPR/nuclease in marmoset embryos were examined. CRISPR/nuclease was more effective than CRISPR/mRNA in avoiding mosai...
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