Ljiljana Paša-Tolić
Environmental Molecular Sciences Laboratory
Publications 182
#1Maowei Dou (Environmental Molecular Sciences Laboratory)H-Index: 2
#2Geremy Clair (PNNL: Pacific Northwest National Laboratory)H-Index: 10
Last.Ying Zhu (Environmental Molecular Sciences Laboratory)H-Index: 15
view all 17 authors...
Effective extension of mass spectrometry-based proteomics to single cells remains challenging. Herein we combined microfluidic nanodroplet technology with tandem mass tag (TMT) isobaric labeling to significantly improve analysis throughput and proteome coverage for single mammalian cells. Isobaric labeling facilitated multiplex analysis of single cell-sized protein quantities to a depth of ∼1 600 proteins with a median CV of 10.9% and correlation coefficient of 0.98. To demonstrate in-depth high...
1 CitationsSource
#1Lloyd M. Smith (UW: University of Wisconsin-Madison)H-Index: 65
#2Paul Martin Thomas (NU: Northwestern University)H-Index: 34
Last.Neil L. Kelleher (NU: Northwestern University)H-Index: 74
view all 21 authors...
To the editor The term proteoform, introduced in Nature Methods in 2013 (ref. 1), has rapidly gained acceptance in the proteomics community. The challenge and importance of comprehensively identifying proteoforms in complex samples has been recognized, and reports have begun to appear of new platforms towards that end2,3,4,5. However, one interesting central ambiguity has emerged, namely determining precisely what is meant by a ‘proteoform identification’. At present, the only practical approach...
#1Mowei Zhou (Environmental Molecular Sciences Laboratory)H-Index: 12
#2Neha Malhan (Environmental Molecular Sciences Laboratory)H-Index: 1
Last.Ljiljana Paša-Tolić (Environmental Molecular Sciences Laboratory)H-Index: 42
view all 14 authors...
Abstract Sorghum [Sorghum bicolor (L.) Moench] is an important cereal crop noted for its ability to survive water-limiting conditions. Herein, we present an analytical workflow to explore the changes in histone modifications through plant developmental stages and two drought stresses in two sorghum genotypes that differ in their response to drought. Top-down mass spectrometry (MS) is an ideal method to profile histone modifications and distinguish closely related histone proteoforms. We analyzed...
#1Beverly J. Agtuca (MU: University of Missouri)H-Index: 4
#2Sylwia A. Stopka (GW: George Washington University)H-Index: 5
Last.Gary Stacey (MU: University of Missouri)H-Index: 74
view all 13 authors...
#1Daniel P. Donnelly (NU: Northeastern University)H-Index: 2
#2Catherine M. Rawlins (NU: Northeastern University)H-Index: 1
Last.Jeffrey N. Agar (NU: Northeastern University)H-Index: 25
view all 28 authors...
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using ...
5 CitationsSource
#1Gary Stacey (MU: University of Missouri)H-Index: 74
#2Akos Vertes (GW: George Washington University)H-Index: 42
Last.David W. Koppenaal (PNNL: Pacific Northwest National Laboratory)H-Index: 25
view all 5 authors...
#1Manda ĆurićH-Index: 9
#2Darko BabićH-Index: 13
Last.Ljerka Tušek-BožićH-Index: 11
view all 6 authors...
#1Sylwia A. Stopka (GW: George Washington University)H-Index: 5
#2Laith Z. Samarah (GW: George Washington University)H-Index: 1
Last.Akos Vertes (GW: George Washington University)H-Index: 42
view all 12 authors...
Mass spectrometry (MS) is an indispensable analytical tool to capture the array of metabolites within complex biological systems. However, conventional MS-based metabolomic workflows require extensive sample processing and separation resulting in limited throughput and potential alteration of the native molecular states in these systems. Ambient ionization methods, capable of sampling directly from tissues, circumvent some of these issues but require high-performance MS to resolve the molecular ...
2 CitationsSource
#1Albert Rivas-Ubach (PNNL: Pacific Northwest National Laboratory)H-Index: 14
#2Yina Liu (A&M: Texas A&M University)H-Index: 9
Last.Alex Guenther (UCI: University of California, Irvine)H-Index: 86
view all 11 authors...
Aerosol particles play important roles in processes controlling the composition of the atmosphere and function of ecosystems. A better understanding of the composition of aerosol particles is beginning to be recognized as critical for ecological research to further comprehend the link between aerosols and ecosystems. While chemical characterization of aerosols has been practiced in the atmospheric science community, detailed methodology tailored to the needs of ecological research does not exist...
1 CitationsSource
#1Yufeng Shen (PNNL: Pacific Northwest National Laboratory)H-Index: 55
#2Rui Zhao (Environmental Molecular Sciences Laboratory)H-Index: 33
Last.Nancy J. Hess (Environmental Molecular Sciences Laboratory)H-Index: 30
view all 8 authors...
We report a novel technical approach for subcritical fluid extraction (SFE) for organic matter characterization in complex matrices such as soil. The custom platform combines on-line SFE with micro-solid phase extraction, nano liquid chromatography (LC), electrospray ionization and Fourier transform mass spectrometry (SFE-LC-FTMS). We demonstrated the utility of SFE-LC-FTMS, including results from both Orbitrap and FTICR MS, for analysis of complex mixtures of organic compounds in a solid matrix...