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Ljiljana Paša-Tolić
Environmental Molecular Sciences Laboratory
Analytical chemistryMass spectrometryFourier transform ion cyclotron resonanceChemistryChromatography
242Publications
48H-index
8,124Citations
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Publications 238
Newest
#1Sarah M. WilliamsH-Index: 1
#2Andrey V. LiyuH-Index: 7
Last. Ryan T. KellyH-Index: 30
view all 12 authors...
Single-cell proteomics can provide critical biological insight into the cellular heterogeneity that is masked by bulk-scale analysis. We have developed a nanoPOTS (nanodroplet processing in one pot for trace samples) platform and demonstrated its broad applicability for single-cell proteomics. However, because of nanoliter-scale sample volumes, the nanoPOTS platform is not compatible with automated LC-MS systems, which significantly limits sample throughput and robustness. To address this challe...
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#1Beverly J. Agtuca (MU: University of Missouri)H-Index: 6
#2Sylwia A. Stopka (GW: George Washington University)H-Index: 7
Last. Christopher R. Anderton (Environmental Molecular Sciences Laboratory)H-Index: 13
view all 12 authors...
: The establishment of the nitrogen-fixing symbiosis between soybean and Bradyrhizobium japonicum is a complex process. In order to document the changes in plant metabolism due to the symbiosis, we utilized laser ablation electrospray ionization mass spectrometry (LAESI-MS) for in situ metabolic profiling of wild-type nodules, nodules infected with a B. japonicum nifH mutant unable to fix nitrogen, nodules doubly infected by both strains, and nodules formed on plants mutated in the stearoyl-acyl...
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#1Mowei Zhou (Environmental Molecular Sciences Laboratory)H-Index: 12
#2Naomi Uwugiaren (University of Gdańsk)H-Index: 2
Last. Ying Zhu (Environmental Molecular Sciences Laboratory)H-Index: 19
view all 9 authors...
Top-down proteomics is a powerful tool for characterizing genetic variations and post-translational modifications at intact protein level. However, one significant technical gap of top-down proteom...
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#1Laith Z. Samarah (GW: George Washington University)H-Index: 1
#2Rikkita Khattar (GW: George Washington University)H-Index: 1
Last. Gary Stacey (MU: University of Missouri)H-Index: 75
view all 14 authors...
Characterization of the metabolic heterogeneity in cell populations requires the analysis of single cells. Most current methods in single-cell analysis rely on cell manipulation, potentially altering the abundance of metabolites in individual cells. Small sample volume and the chemical diversity of metabolites are additional challenges in single-cell metabolomics. Here, we describe the combination of fiber-based laser ablation electrospray ionization (f-LAESI) with 21 tesla Fourier transform ion...
1 CitationsSource
#1Chia-Feng Tsai (PNNL: Pacific Northwest National Laboratory)H-Index: 14
#2Rui Zhao (PNNL: Pacific Northwest National Laboratory)H-Index: 35
Last. Tujin Shi (PNNL: Pacific Northwest National Laboratory)H-Index: 19
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Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective processing of single cells. In particular, the concept of using boosting/carrier channels in isobaric labeling to increase the sensitivity in MS detection has also been increasingly used for quantitative p...
4 CitationsSource
#1Dušan Veličković (Environmental Molecular Sciences Laboratory)H-Index: 11
#2Guanshi Zhang (University of Texas at Austin)H-Index: 3
Last. Christopher R. Anderton (University of Texas at Austin)
view all 8 authors...
Automated spraying devices have become ubiquitous in laboratories employing matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), in part because they permit control of a number of matrix application parameters that can easily be reproduced for intra- and interlaboratory studies. Determining the optimal parameters for MALDI matrix application, such as temperature, flow rate, spraying velocity, number of spraying cycles, and solvent composition for matrix application,...
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#1Beverly J. Agtuca (MU: University of Missouri)H-Index: 6
#2Sylwia A. Stopka (GW: George Washington University)H-Index: 7
Last. Gary Stacey (MU: University of Missouri)H-Index: 75
view all 13 authors...
: Over the past decades, crop yields have risen in parallel with increasing use of fossil fuel-derived nitrogen (N) fertilizers but with concomitant negative impacts on climate and water resources. There is a need for more sustainable agricultural practices, and biological nitrogen fixation (BNF) could be part of the solution. A variety of nitrogen-fixing, epiphytic, and endophytic plant growth-promoting bacteria (PGPB) are known to stimulate plant growth. However, compared with the rhizobium-le...
2 CitationsSource
#1Live Heldal Hagen (NMBU: Norwegian University of Life Sciences)H-Index: 10
#2Charles Garrett Brooke (UC Davis: University of California, Davis)H-Index: 2
Last. Matthias Hess (UC Davis: University of California, Davis)H-Index: 17
view all 20 authors...
The rumen harbors a complex microbial mixture of archaea, bacteria, protozoa and fungi that efficiently breakdown plant biomass and its complex dietary carbohydrates into soluble sugars that can be fermented and subsequently converted into metabolites and nutrients utilized by the host animal. While rumen bacterial populations have been well documented, only a fraction of the rumen eukarya are taxonomically and functionally characterized, despite the recognition that they contribute to the cellu...
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#1Heather S. Smallwood (UTHSC: University of Tennessee Health Science Center)H-Index: 16
#1Svetlana Rezinciuc (UTHSC: University of Tennessee Health Science Center)H-Index: 1
Last. Paul G. ThomasH-Index: 45
view all 12 authors...
Infection with the influenza virus triggers an innate immune response aimed at initiating the adaptive response to halt viral replication and spread. However, the metabolic response fueling the molecular mechanisms underlying changes in innate immune cell homeostasis remain undefined. Thus, we compared the metabolic response of dendritic cells to that of those infected with active and inactive influenza A virus or treated with toll like receptor agonists. While influenza infects dendritic cells,...
1 CitationsSource
#1Mowei Zhou (Environmental Molecular Sciences Laboratory)H-Index: 12
#2Neha Malhan (Environmental Molecular Sciences Laboratory)H-Index: 1
Last. Ljiljana Paša-Tolić (Environmental Molecular Sciences Laboratory)H-Index: 48
view all 14 authors...
Abstract Sorghum [Sorghum bicolor (L.) Moench] is an important cereal crop noted for its ability to survive water-limiting conditions. Herein, we present an analytical workflow to explore the changes in histone modifications through plant developmental stages and two drought stresses in two sorghum genotypes that differ in their response to drought. Top-down mass spectrometry (MS) is an ideal method to profile histone modifications and distinguish closely related histone proteoforms. We analyzed...
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