Julien Gravier
Beth Israel Deaconess Medical Center
NanoparticleNanotechnologyChemistryIn vivoFluorescence-lifetime imaging microscopy
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Publications 25
#1Hideyuki Wada (Hokkaido University)H-Index: 8
#2Hoon Hyun (Chonnam National University)H-Index: 22
Last. John V. Frangioni (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 61
view all 8 authors...
Background There are currently no thymus-specific contrast agents for biomedical imaging. Thus, finding ectopic thymic tissue during certain operations is extremely difficult. The purpose of the present study was to determine if near-infrared (NIR) fluorescence imaging could provide high sensitivity, real-time identification of thymic tissue during the operation. Methods After initial in vivo screening of a 315-compound NIR fluorophore library for thymic uptake, methylene blue and five different...
2 CitationsSource
#1Homan Kang (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 20
#2Julien Gravier (French Institute of Health and Medical Research)H-Index: 15
Last. Hak Soo Choi (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 41
view all 11 authors...
Abstract Renally cleared zwitterionic nanocarriers (H-Dots) are composed of e-polylysine backbone for charge variations, near-infrared fluorophores for bioimaging, and β-cyclodextrins for potential drug delivery. H-Dots show ideal systemic circulation and rapid distribution and excrete from normal tissue/organ via renal excretion after complete targeting to the tumor site without nonspecific uptake by the immune system.
39 CitationsSource
#1Anastassia Karageorgis (UJF: Joseph Fourier University)H-Index: 3
#2Sandrine Dufort (UJF: Joseph Fourier University)H-Index: 21
Last. Jean-Luc Coll (UJF: Joseph Fourier University)H-Index: 39
view all 14 authors...
Nanoparticles are useful tools in oncology because of their capacity to passively accumulate in tumors in particular via the enhanced permeability and retention (EPR) effect. However, the importance and reliability of this effect remains controversial and quite often unpredictable. In this preclinical study, we used optical imaging to detect the accumulation of three types of fluorescent nanoparticles in eight different subcutaneous and orthotopic tumor models, and dynamic contrast-enhanced and ...
17 CitationsSource
#1Hideyuki Wada (Hokkaido University)H-Index: 8
#2Hoon Hyun (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 22
Last. Hak Soo Choi (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 41
view all 8 authors...
Background Although the sentinel lymph node (SLN) hypothesis has been applied to many tissues and organs, liver has remained unstudied. Currently, it is unclear whether hepatic SLNs even exist. If so, they could alter the management of intrahepatic cholangiocarcinoma and other hepatic malignancies by minimizing the extent of surgery while still providing precise nodal staging. This study investigated whether invisible yet tissue-penetrating near-infrared (NIR) fluorescent light can provide simul...
8 CitationsSource
#1Kai Bao (SPU: Shenyang Pharmaceutical University)H-Index: 16
#2Khaled Nasr (Harvard University)H-Index: 13
Last. Hak Soo Choi (Harvard University)H-Index: 41
view all 7 authors...
Recent advances in near-infrared (NIR) fluorescence imaging enabled real-time intraoperative detection of bone metastases, bone growth, and tissue microcalcification. Pamidronate (PAM) has been widely used for this purpose because of its high binding affinity toward bone and remarkable therapeutic effects. Herein we describe the development of a series of PAM-conjugated NIR fluorophores that varied in net charges and hydrophobicity, and compared their bone targeting efficiency, biodistribution, ...
15 CitationsSource
#1Hideyuki WadaH-Index: 8
#2Hoon HyunH-Index: 22
Last. Hak Soo ChoiH-Index: 41
view all 9 authors...
Objective: Pancreas-related complications are some of the most serious ones in abdominal surgery. The goal of this study was to develop and validate novel near-infrared (NIR) fluorophores that would enable real-time pancreas imaging to avoid the intraoperative pancreatic injury. Design: After initial screening of a large NIR fluorophore library, the performance of 3 selected pancreas-targeted 700 nm NIR fluorophores, T700-H, T700-F, and MB, were quantified in mice, rats, and pigs. Dose ranging u...
28 CitationsSource
#1Hoon Hyun (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 22
#2Eric A. Owens (GSU: Georgia State University)H-Index: 16
Last. Maged Henary (GSU: Georgia State University)H-Index: 27
view all 9 authors...
Functional near-infrared (NIR) fluorophores have played a major role in the recent advances in bioimaging. However, the optical and physicochemical stabilities of NIR fluorophores in the biological and physiological environment are still a challenge. Especially, the ether linkage on the meso carbon of heptamethine core is fragile when exposed to serum proteins or other amine-rich biomolecules. To solve such a structural limitation, a rigid carbon–carbon bond was installed onto the framework of e...
16 CitationsSource
#1Hoon Hyun (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 22
#2Hideyuki Wada (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 8
Last. Hak Soo Choi (PNU: Pusan National University)H-Index: 41
view all 9 authors...
The conventional method for creating targeted contrast agents is to conjugate separate targeting and fluorophore domains. A new strategy is based on the incorporation of targeting moieties into the non-delocalized structure of pentamethine and heptamethine indocyanines. Using the known affinity of phosphonates for bone minerals in a model system, two families of bifunctional molecules that target bone without requiring a traditional bisphosphonate are synthesized. With peak fluorescence emission...
49 CitationsSource
#1Julien GravierH-Index: 15
#2Lucie SanceyH-Index: 26
Last. Isabelle TexierH-Index: 19
view all 8 authors...
DiI and DiD, two fluorophores able to interact by FRET (Forster resonance energy transfer), were coencapsulated in the core of lipid nanocapsules (LNCs) and nanoemulsions (LNEs), lipophilic reservoirs for the delivery of drugs. The ability of FRET imaging to provide information on the kinetics of dissociation of the nanoparticles in the presence of bovine serum albumin (BSA) or whole serum, or after incubation with cancer cells, and after systemic administration in tumor-bearing mice, was studie...
30 CitationsSource
#1Anne-Laure Lainé (French Institute of Health and Medical Research)H-Index: 6
#2Julien Gravier (French Institute of Health and Medical Research)H-Index: 15
Last. Catherine Passirani (French Institute of Health and Medical Research)H-Index: 38
view all 11 authors...
Abstract The determination of the nanocarrier fate in preclinical models is required before any translation from laboratory to clinical trials. Modern fluorescent imaging techniques have gained considerable advances becoming a powerful technology for non-invasive visualization in living subjects. Among them, Forster (fluorescence) resonance energy transfer (FRET) is a particular fluorescence imaging which involves energy transfer between 2 fluorophores in a distance-dependent manner. Considering...
36 CitationsSource